Spatio-temporal variability and diversity of water column prokaryotic communities in the eastern North Atlantic
This work describes the microbial community structure in the water column at the Porcupine Abyssal Plain in the eastern North Atlantic. Restriction fragment length polymorphism analysis was carried out on clone libraries constructed from samples collected at 100 m, 1000 m, 3000 m, 10 m above bottom...
Published in: | FEMS Microbiology Ecology |
---|---|
Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
Oxford University Press
2004
|
Subjects: | |
Online Access: | http://femsec.oxfordjournals.org/cgi/content/short/47/2/249 https://doi.org/10.1016/S0168-6496(03)00281-2 |
id |
fthighwire:oai:open-archive.highwire.org:femsec:47/2/249 |
---|---|
record_format |
openpolar |
spelling |
fthighwire:oai:open-archive.highwire.org:femsec:47/2/249 2023-05-15T17:31:08+02:00 Spatio-temporal variability and diversity of water column prokaryotic communities in the eastern North Atlantic Gallagher, Joseph M Carton, Micheal W Eardly, Donal F Patching, John W 2004-02-01 00:00:00.0 text/html http://femsec.oxfordjournals.org/cgi/content/short/47/2/249 https://doi.org/10.1016/S0168-6496(03)00281-2 en eng Oxford University Press http://femsec.oxfordjournals.org/cgi/content/short/47/2/249 http://dx.doi.org/10.1016/S0168-6496(03)00281-2 Copyright (C) 2004, Oxford University Press Articles TEXT 2004 fthighwire https://doi.org/10.1016/S0168-6496(03)00281-2 2015-03-01T01:14:32Z This work describes the microbial community structure in the water column at the Porcupine Abyssal Plain in the eastern North Atlantic. Restriction fragment length polymorphism analysis was carried out on clone libraries constructed from samples collected at 100 m, 1000 m, 3000 m, 10 m above bottom and sediment contact water during July 1997 and March 1998. Simpson (1/ D ) and Shannon ( H ′) diversity indices revealed temporal and spatial variations in the community structure and complexity. Higher diversity was observed in the samples collected from 100 m ( H ′=3.22), 1000 m ( H ′=3.48) and 10 m above bottom ( H ′=3.18) during July 1997 compared with the corresponding samples during March 1998. Changes in diversity may be associated with a seasonal flux in particulate organic matter. This could promote the proliferation of a selection of taxa that are adapted to rapidly responding to a large influx of organic matter. Sequencing of clones representing 20 operational taxonomic units revealed a diverse population of Bacteria and Archaea. The most numerous clone operational taxonomic units were from the α and γ subdivisions of the Proteobacteria and the group I marine Crenarchaeota. A number of sequences were phylogenetically grouped in clades with no culture representatives such as the SAR116, SAR86, SAR406 and SAR324 groups. Most of the sequences identified were found to be more closely related to other 16S rDNA clones recovered from the marine environment rather than cultured species. Text North Atlantic HighWire Press (Stanford University) FEMS Microbiology Ecology 47 2 249 262 |
institution |
Open Polar |
collection |
HighWire Press (Stanford University) |
op_collection_id |
fthighwire |
language |
English |
topic |
Articles |
spellingShingle |
Articles Gallagher, Joseph M Carton, Micheal W Eardly, Donal F Patching, John W Spatio-temporal variability and diversity of water column prokaryotic communities in the eastern North Atlantic |
topic_facet |
Articles |
description |
This work describes the microbial community structure in the water column at the Porcupine Abyssal Plain in the eastern North Atlantic. Restriction fragment length polymorphism analysis was carried out on clone libraries constructed from samples collected at 100 m, 1000 m, 3000 m, 10 m above bottom and sediment contact water during July 1997 and March 1998. Simpson (1/ D ) and Shannon ( H ′) diversity indices revealed temporal and spatial variations in the community structure and complexity. Higher diversity was observed in the samples collected from 100 m ( H ′=3.22), 1000 m ( H ′=3.48) and 10 m above bottom ( H ′=3.18) during July 1997 compared with the corresponding samples during March 1998. Changes in diversity may be associated with a seasonal flux in particulate organic matter. This could promote the proliferation of a selection of taxa that are adapted to rapidly responding to a large influx of organic matter. Sequencing of clones representing 20 operational taxonomic units revealed a diverse population of Bacteria and Archaea. The most numerous clone operational taxonomic units were from the α and γ subdivisions of the Proteobacteria and the group I marine Crenarchaeota. A number of sequences were phylogenetically grouped in clades with no culture representatives such as the SAR116, SAR86, SAR406 and SAR324 groups. Most of the sequences identified were found to be more closely related to other 16S rDNA clones recovered from the marine environment rather than cultured species. |
format |
Text |
author |
Gallagher, Joseph M Carton, Micheal W Eardly, Donal F Patching, John W |
author_facet |
Gallagher, Joseph M Carton, Micheal W Eardly, Donal F Patching, John W |
author_sort |
Gallagher, Joseph M |
title |
Spatio-temporal variability and diversity of water column prokaryotic communities in the eastern North Atlantic |
title_short |
Spatio-temporal variability and diversity of water column prokaryotic communities in the eastern North Atlantic |
title_full |
Spatio-temporal variability and diversity of water column prokaryotic communities in the eastern North Atlantic |
title_fullStr |
Spatio-temporal variability and diversity of water column prokaryotic communities in the eastern North Atlantic |
title_full_unstemmed |
Spatio-temporal variability and diversity of water column prokaryotic communities in the eastern North Atlantic |
title_sort |
spatio-temporal variability and diversity of water column prokaryotic communities in the eastern north atlantic |
publisher |
Oxford University Press |
publishDate |
2004 |
url |
http://femsec.oxfordjournals.org/cgi/content/short/47/2/249 https://doi.org/10.1016/S0168-6496(03)00281-2 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
http://femsec.oxfordjournals.org/cgi/content/short/47/2/249 http://dx.doi.org/10.1016/S0168-6496(03)00281-2 |
op_rights |
Copyright (C) 2004, Oxford University Press |
op_doi |
https://doi.org/10.1016/S0168-6496(03)00281-2 |
container_title |
FEMS Microbiology Ecology |
container_volume |
47 |
container_issue |
2 |
container_start_page |
249 |
op_container_end_page |
262 |
_version_ |
1766128480132530176 |