Distribution of culturable microorganisms in Fennoscandian Shield groundwater
Microbial populations in 16 groundwater samples from six Fennoscandian Shield sites in Finland and Sweden were investigated. The average total cell number was 3.7×105 cells ml−1, and there was no change in the mean of the total cell numbers to a depth of 1390 m. Culture media were designed based on...
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fthighwire:oai:open-archive.highwire.org:femsec:39/2/129 2023-05-15T16:12:49+02:00 Distribution of culturable microorganisms in Fennoscandian Shield groundwater Haveman, Shelley A. Pedersen, Karsten 2002-02-01 00:00:00.0 text/html http://femsec.oxfordjournals.org/cgi/content/short/39/2/129 https://doi.org/10.1111/j.1574-6941.2002.tb00914.x en eng Oxford University Press http://femsec.oxfordjournals.org/cgi/content/short/39/2/129 http://dx.doi.org/10.1111/j.1574-6941.2002.tb00914.x Copyright (C) 2002, Oxford University Press Articles TEXT 2002 fthighwire https://doi.org/10.1111/j.1574-6941.2002.tb00914.x 2016-11-16T18:58:13Z Microbial populations in 16 groundwater samples from six Fennoscandian Shield sites in Finland and Sweden were investigated. The average total cell number was 3.7×105 cells ml−1, and there was no change in the mean of the total cell numbers to a depth of 1390 m. Culture media were designed based on the chemical composition of each groundwater sample and used successfully to culture anaerobic microorganisms from all samples between 65 and 1350 m depth. Between 0.0084 and 14.8% of total cells were cultured from groundwater samples. Sulfate-reducing bacteria, iron-reducing bacteria and heterotrophic acetogenic bacteria were cultured from groundwater sampled at 65–686 m depth in geographically distant sites. Different microbial populations were cultured from deeper, older and more saline groundwater from 863 to 1350 m depth. Principal component analysis of groundwater chemistry data showed that sulfate- and iron-reducing bacteria were not detected in the most saline groundwater. Iron-reducing bacteria and acetogens were cultured from deep groundwater that contained 0.35–3.5 mM sulfate, while methanogens and acetogens were cultured from deep sulfate-depleted groundwater. In one borehole from which autotrophic methanogens were cultured, dissolved inorganic carbon was enriched in 13C compared to other Fennoscandian Shield groundwater samples, suggesting that autotrophs were active. It can be concluded that a diverse microbial community is present from the surface to over 1300 m depth in the Fennoscandian Shield. Text Fennoscandian HighWire Press (Stanford University) FEMS Microbiology Ecology 39 2 129 137 |
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Articles Haveman, Shelley A. Pedersen, Karsten Distribution of culturable microorganisms in Fennoscandian Shield groundwater |
topic_facet |
Articles |
description |
Microbial populations in 16 groundwater samples from six Fennoscandian Shield sites in Finland and Sweden were investigated. The average total cell number was 3.7×105 cells ml−1, and there was no change in the mean of the total cell numbers to a depth of 1390 m. Culture media were designed based on the chemical composition of each groundwater sample and used successfully to culture anaerobic microorganisms from all samples between 65 and 1350 m depth. Between 0.0084 and 14.8% of total cells were cultured from groundwater samples. Sulfate-reducing bacteria, iron-reducing bacteria and heterotrophic acetogenic bacteria were cultured from groundwater sampled at 65–686 m depth in geographically distant sites. Different microbial populations were cultured from deeper, older and more saline groundwater from 863 to 1350 m depth. Principal component analysis of groundwater chemistry data showed that sulfate- and iron-reducing bacteria were not detected in the most saline groundwater. Iron-reducing bacteria and acetogens were cultured from deep groundwater that contained 0.35–3.5 mM sulfate, while methanogens and acetogens were cultured from deep sulfate-depleted groundwater. In one borehole from which autotrophic methanogens were cultured, dissolved inorganic carbon was enriched in 13C compared to other Fennoscandian Shield groundwater samples, suggesting that autotrophs were active. It can be concluded that a diverse microbial community is present from the surface to over 1300 m depth in the Fennoscandian Shield. |
format |
Text |
author |
Haveman, Shelley A. Pedersen, Karsten |
author_facet |
Haveman, Shelley A. Pedersen, Karsten |
author_sort |
Haveman, Shelley A. |
title |
Distribution of culturable microorganisms in Fennoscandian Shield groundwater |
title_short |
Distribution of culturable microorganisms in Fennoscandian Shield groundwater |
title_full |
Distribution of culturable microorganisms in Fennoscandian Shield groundwater |
title_fullStr |
Distribution of culturable microorganisms in Fennoscandian Shield groundwater |
title_full_unstemmed |
Distribution of culturable microorganisms in Fennoscandian Shield groundwater |
title_sort |
distribution of culturable microorganisms in fennoscandian shield groundwater |
publisher |
Oxford University Press |
publishDate |
2002 |
url |
http://femsec.oxfordjournals.org/cgi/content/short/39/2/129 https://doi.org/10.1111/j.1574-6941.2002.tb00914.x |
genre |
Fennoscandian |
genre_facet |
Fennoscandian |
op_relation |
http://femsec.oxfordjournals.org/cgi/content/short/39/2/129 http://dx.doi.org/10.1111/j.1574-6941.2002.tb00914.x |
op_rights |
Copyright (C) 2002, Oxford University Press |
op_doi |
https://doi.org/10.1111/j.1574-6941.2002.tb00914.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
39 |
container_issue |
2 |
container_start_page |
129 |
op_container_end_page |
137 |
_version_ |
1765998429050241024 |