Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.)
Colonisation and transmission of Aeromonas salmonicida in Atlantic salmon was investigated using wild-type and lux -marked strains of A. salmonicida . An initial intra-peritoneal (i.p.) challenge showed that lux -marked cells were virulent only when injected at concentrations ≥109 cfu ml−1 and signi...
Published in: | FEMS Microbiology Ecology |
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1998
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fthighwire:oai:open-archive.highwire.org:femsec:27/3/251 2023-05-15T15:31:47+02:00 Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.) Ferguson, Yvonne Bricknell, Ian R. Glover, L. Anne MacGregor, Doreen M Prosser, Jim I. 1998-11-01 00:00:00.0 text/html http://femsec.oxfordjournals.org/cgi/content/short/27/3/251 https://doi.org/10.1111/j.1574-6941.1998.tb00541.x en eng Oxford University Press http://femsec.oxfordjournals.org/cgi/content/short/27/3/251 http://dx.doi.org/10.1111/j.1574-6941.1998.tb00541.x Copyright (C) 1998, Oxford University Press Articles TEXT 1998 fthighwire https://doi.org/10.1111/j.1574-6941.1998.tb00541.x 2016-11-16T16:54:51Z Colonisation and transmission of Aeromonas salmonicida in Atlantic salmon was investigated using wild-type and lux -marked strains of A. salmonicida . An initial intra-peritoneal (i.p.) challenge showed that lux -marked cells were virulent only when injected at concentrations ≥109 cfu ml−1 and significantly less infective than wild-type MT463. The low virulence of A. salmonicida MT463 lux AB was probably due to loss of the proteinaceous A-layer, which is an important virulence factor involved in both intra- and inter-cellular A. salmonicida interactions. During the i.p. challenge, all fish were held in one tank enabling assessment of transfer of lux -marked A. salmonicida between fish. Lux -marked cells shed from moribund and dead fish survived in the water column and cross-infected cohabitant fish. Cross-infection by A. salmonicida MT463 lux AB was investigated further by carrying out a cohabitation challenge. Lux -marked cells were recovered in low numbers from gill tissue and skin/mucus of cohabitant fish. Poor adhesion of cells may be due to loss of the A-layer protein. During a second cohabitation challenge using A-layer+ and virulent wild-type strain MT432, between 102 and 107 cells g−1 of fish gill tissue or skin/mucus were isolated. This result confirmed the preliminary observations obtained using lux -marked A. salmonicida MT463 and suggested that the gill and skin/mucus regions of fish were the main sites for attachment of A. salmonicida . None of the A. salmonicida strains was recovered from fish intestine samples during cohabitation challenges. Text Atlantic salmon Salmo salar HighWire Press (Stanford University) FEMS Microbiology Ecology 27 3 251 260 |
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Articles Ferguson, Yvonne Bricknell, Ian R. Glover, L. Anne MacGregor, Doreen M Prosser, Jim I. Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.) |
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Articles |
description |
Colonisation and transmission of Aeromonas salmonicida in Atlantic salmon was investigated using wild-type and lux -marked strains of A. salmonicida . An initial intra-peritoneal (i.p.) challenge showed that lux -marked cells were virulent only when injected at concentrations ≥109 cfu ml−1 and significantly less infective than wild-type MT463. The low virulence of A. salmonicida MT463 lux AB was probably due to loss of the proteinaceous A-layer, which is an important virulence factor involved in both intra- and inter-cellular A. salmonicida interactions. During the i.p. challenge, all fish were held in one tank enabling assessment of transfer of lux -marked A. salmonicida between fish. Lux -marked cells shed from moribund and dead fish survived in the water column and cross-infected cohabitant fish. Cross-infection by A. salmonicida MT463 lux AB was investigated further by carrying out a cohabitation challenge. Lux -marked cells were recovered in low numbers from gill tissue and skin/mucus of cohabitant fish. Poor adhesion of cells may be due to loss of the A-layer protein. During a second cohabitation challenge using A-layer+ and virulent wild-type strain MT432, between 102 and 107 cells g−1 of fish gill tissue or skin/mucus were isolated. This result confirmed the preliminary observations obtained using lux -marked A. salmonicida MT463 and suggested that the gill and skin/mucus regions of fish were the main sites for attachment of A. salmonicida . None of the A. salmonicida strains was recovered from fish intestine samples during cohabitation challenges. |
format |
Text |
author |
Ferguson, Yvonne Bricknell, Ian R. Glover, L. Anne MacGregor, Doreen M Prosser, Jim I. |
author_facet |
Ferguson, Yvonne Bricknell, Ian R. Glover, L. Anne MacGregor, Doreen M Prosser, Jim I. |
author_sort |
Ferguson, Yvonne |
title |
Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.) |
title_short |
Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.) |
title_full |
Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.) |
title_fullStr |
Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.) |
title_full_unstemmed |
Colonisation and transmission of lux-marked and wild-type Aeromonas salmonicida strains in Atlantic salmon (Salmo salar L.) |
title_sort |
colonisation and transmission of lux-marked and wild-type aeromonas salmonicida strains in atlantic salmon (salmo salar l.) |
publisher |
Oxford University Press |
publishDate |
1998 |
url |
http://femsec.oxfordjournals.org/cgi/content/short/27/3/251 https://doi.org/10.1111/j.1574-6941.1998.tb00541.x |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
http://femsec.oxfordjournals.org/cgi/content/short/27/3/251 http://dx.doi.org/10.1111/j.1574-6941.1998.tb00541.x |
op_rights |
Copyright (C) 1998, Oxford University Press |
op_doi |
https://doi.org/10.1111/j.1574-6941.1998.tb00541.x |
container_title |
FEMS Microbiology Ecology |
container_volume |
27 |
container_issue |
3 |
container_start_page |
251 |
op_container_end_page |
260 |
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1766362303855329280 |