Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades
Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO 2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been repo...
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fthighwire:oai:open-archive.highwire.org:biolopen:bio.2011036v1 2023-05-15T17:51:24+02:00 Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades Nakamura, Masako Morita, Masaya Kurihara, Haruko Mitarai, Satoshi 2011-11-03 07:40:16.0 text/html http://bio.biologists.org/cgi/content/short/bio.2011036v1 https://doi.org/10.1242/bio.2011036 en eng Company of Biologists http://bio.biologists.org/cgi/content/short/bio.2011036v1 http://dx.doi.org/10.1242/bio.2011036 Copyright (C) 2011, Company of Biologists Research Article TEXT 2011 fthighwire https://doi.org/10.1242/bio.2011036 2015-02-28T22:50:17Z Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO 2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO 2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsp s and hsf1 by quantitative real‐time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO 2 conditions. These findings indicate that the expression of hsp s is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression. Text Ocean acidification HighWire Press (Stanford University) Biology Open 1 2 75 81 |
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Research Article Nakamura, Masako Morita, Masaya Kurihara, Haruko Mitarai, Satoshi Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades |
topic_facet |
Research Article |
description |
Ocean acidification is an ongoing threat for marine organisms due to the increasing atmospheric CO 2 concentration. Seawater acidification has a serious impact on physiologic processes in marine organisms at all life stages. On the other hand, potential tolerance to external pH changes has been reported in coral larvae. Information about the possible mechanisms underlying such tolerance responses, however, is scarce. In the present study, we examined the effects of acidified seawater on the larvae of Acropora digitifera at the molecular level. We targeted two heat shock proteins, Hsp70 and Hsp90, and a heat shock transcription factor, Hsf1, because of their importance in stress responses and in early life developmental stages. Coral larvae were maintained under the ambient and elevated CO 2 conditions that are expected to occur within next 100 years, and then we evaluated the expression of hsp s and hsf1 by quantitative real‐time polymerase chain reaction (PCR). Expression levels of these molecules significantly differed among target genes, but they did not change significantly between CO 2 conditions. These findings indicate that the expression of hsp s is not changed due to external pH changes, and suggest that tolerance to acidified seawater in coral larvae may not be related to hsp expression. |
format |
Text |
author |
Nakamura, Masako Morita, Masaya Kurihara, Haruko Mitarai, Satoshi |
author_facet |
Nakamura, Masako Morita, Masaya Kurihara, Haruko Mitarai, Satoshi |
author_sort |
Nakamura, Masako |
title |
Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades |
title_short |
Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades |
title_full |
Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades |
title_fullStr |
Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades |
title_full_unstemmed |
Expression of hsp70, hsp90 and hsf1 in the reef coral Acropora digitifera under prospective acidified conditions over the next several decades |
title_sort |
expression of hsp70, hsp90 and hsf1 in the reef coral acropora digitifera under prospective acidified conditions over the next several decades |
publisher |
Company of Biologists |
publishDate |
2011 |
url |
http://bio.biologists.org/cgi/content/short/bio.2011036v1 https://doi.org/10.1242/bio.2011036 |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
http://bio.biologists.org/cgi/content/short/bio.2011036v1 http://dx.doi.org/10.1242/bio.2011036 |
op_rights |
Copyright (C) 2011, Company of Biologists |
op_doi |
https://doi.org/10.1242/bio.2011036 |
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Biology Open |
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1 |
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2 |
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75 |
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81 |
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1766158543785820160 |