Gene signatures to prediction rheumatoid arthritis development
Background/purpose Early recognition of development of rheumatoid arthritis (RA) allows timely start of treatment. Recently, the authors reported gene signatures in the peripheral blood (PB), involving interferon (IFN) activity and B cell related genes, which are associated with the development of a...
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fthighwire:oai:open-archive.highwire.org:annrheumdis:71/Suppl_1/A24-a 2023-05-15T17:45:05+02:00 Gene signatures to prediction rheumatoid arthritis development Lubbers, Joyce Brink, Mikael van de Stadt, Lotte A Vosslamber, Saskia Wesseling, John G van Schaardenburg, Dirkjan Rantapaa-Dahlqvist, Solbritt M Verweij, Cornelis L 2012-02-01 00:00:00.0 text/html http://ard.bmj.com/cgi/content/short/71/Suppl_1/A24-a https://doi.org/10.1136/annrheumdis-2011-201231.13 en eng BMJ Publishing Group Ltd http://ard.bmj.com/cgi/content/short/71/Suppl_1/A24-a http://dx.doi.org/10.1136/annrheumdis-2011-201231.13 Copyright (C) 2012, BMJ Publishing Group Ltd 2. Pre-disease-What happens before diagnosis? TEXT 2012 fthighwire https://doi.org/10.1136/annrheumdis-2011-201231.13 2013-05-27T09:30:58Z Background/purpose Early recognition of development of rheumatoid arthritis (RA) allows timely start of treatment. Recently, the authors reported gene signatures in the peripheral blood (PB), involving interferon (IFN) activity and B cell related genes, which are associated with the development of arthritis. The objective of this study is to validate these findings in independent cohorts. Method The authors analysed IFN-response gene expression in PB samples from 40 anticitrullinated protein antibodies (ACPA)+/rheumatoid factor (RF)+arthralgia patients from the Amsterdam Reade cohort who are clinically followed for RA development. In addition, PB mononuclear cells (MCs) of 22 preonset RA, 25 RA patients, and 48 healthy controls (HC) from the Northern Sweden Population Repository (Umeå University) were studied. Fluorescence-activated cell sorting (FACS)-analysis for B cell marker expression was performed on PB from 87 ACPA+/RF+ arthralgia patients and 30 HC from the Amsterdam Reade cohort. Quantitative PCR (qPCR) for B cell marker was performed of 45 ACPA+/RF+ arthralgia patients and six HC from the Amsterdam Reade cohort. Results The IFN-score (average of seven IFN-response genes) was measured at inclusion in an independent group of ACPA+/RF+ arthralgia patients at risk for RA (n=40). A total of 19 patients developed arthritis within a median period of 11 months (IQR 9–28). Comparative analysis of IFN-score high and low patients confirmed that the high IFN-score is associated with conversion to RA (p=0.025). In addition, the authors measured the IFN-score in PBMC from RA and preonset RA patients, and HC from the Northern Swedish Repository. Comparative analyses between the groups revealed that both preonset RA patients as well as RA patients had significantly increased IFN-scores compared to HC (Mann–Whitney U test p=0.0064 and p=0.0075, respectively). For the analysis of the role of B cells in development of RA the authors analysed the FACS profile of 87 ACPA+/RF+ at risk patients at inclusion, of who 22 ... Text Northern Sweden HighWire Press (Stanford University) Annals of the Rheumatic Diseases 71 Suppl 1 A24.1 A24 |
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HighWire Press (Stanford University) |
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English |
topic |
2. Pre-disease-What happens before diagnosis? |
spellingShingle |
2. Pre-disease-What happens before diagnosis? Lubbers, Joyce Brink, Mikael van de Stadt, Lotte A Vosslamber, Saskia Wesseling, John G van Schaardenburg, Dirkjan Rantapaa-Dahlqvist, Solbritt M Verweij, Cornelis L Gene signatures to prediction rheumatoid arthritis development |
topic_facet |
2. Pre-disease-What happens before diagnosis? |
description |
Background/purpose Early recognition of development of rheumatoid arthritis (RA) allows timely start of treatment. Recently, the authors reported gene signatures in the peripheral blood (PB), involving interferon (IFN) activity and B cell related genes, which are associated with the development of arthritis. The objective of this study is to validate these findings in independent cohorts. Method The authors analysed IFN-response gene expression in PB samples from 40 anticitrullinated protein antibodies (ACPA)+/rheumatoid factor (RF)+arthralgia patients from the Amsterdam Reade cohort who are clinically followed for RA development. In addition, PB mononuclear cells (MCs) of 22 preonset RA, 25 RA patients, and 48 healthy controls (HC) from the Northern Sweden Population Repository (Umeå University) were studied. Fluorescence-activated cell sorting (FACS)-analysis for B cell marker expression was performed on PB from 87 ACPA+/RF+ arthralgia patients and 30 HC from the Amsterdam Reade cohort. Quantitative PCR (qPCR) for B cell marker was performed of 45 ACPA+/RF+ arthralgia patients and six HC from the Amsterdam Reade cohort. Results The IFN-score (average of seven IFN-response genes) was measured at inclusion in an independent group of ACPA+/RF+ arthralgia patients at risk for RA (n=40). A total of 19 patients developed arthritis within a median period of 11 months (IQR 9–28). Comparative analysis of IFN-score high and low patients confirmed that the high IFN-score is associated with conversion to RA (p=0.025). In addition, the authors measured the IFN-score in PBMC from RA and preonset RA patients, and HC from the Northern Swedish Repository. Comparative analyses between the groups revealed that both preonset RA patients as well as RA patients had significantly increased IFN-scores compared to HC (Mann–Whitney U test p=0.0064 and p=0.0075, respectively). For the analysis of the role of B cells in development of RA the authors analysed the FACS profile of 87 ACPA+/RF+ at risk patients at inclusion, of who 22 ... |
format |
Text |
author |
Lubbers, Joyce Brink, Mikael van de Stadt, Lotte A Vosslamber, Saskia Wesseling, John G van Schaardenburg, Dirkjan Rantapaa-Dahlqvist, Solbritt M Verweij, Cornelis L |
author_facet |
Lubbers, Joyce Brink, Mikael van de Stadt, Lotte A Vosslamber, Saskia Wesseling, John G van Schaardenburg, Dirkjan Rantapaa-Dahlqvist, Solbritt M Verweij, Cornelis L |
author_sort |
Lubbers, Joyce |
title |
Gene signatures to prediction rheumatoid arthritis development |
title_short |
Gene signatures to prediction rheumatoid arthritis development |
title_full |
Gene signatures to prediction rheumatoid arthritis development |
title_fullStr |
Gene signatures to prediction rheumatoid arthritis development |
title_full_unstemmed |
Gene signatures to prediction rheumatoid arthritis development |
title_sort |
gene signatures to prediction rheumatoid arthritis development |
publisher |
BMJ Publishing Group Ltd |
publishDate |
2012 |
url |
http://ard.bmj.com/cgi/content/short/71/Suppl_1/A24-a https://doi.org/10.1136/annrheumdis-2011-201231.13 |
genre |
Northern Sweden |
genre_facet |
Northern Sweden |
op_relation |
http://ard.bmj.com/cgi/content/short/71/Suppl_1/A24-a http://dx.doi.org/10.1136/annrheumdis-2011-201231.13 |
op_rights |
Copyright (C) 2012, BMJ Publishing Group Ltd |
op_doi |
https://doi.org/10.1136/annrheumdis-2011-201231.13 |
container_title |
Annals of the Rheumatic Diseases |
container_volume |
71 |
container_issue |
Suppl 1 |
container_start_page |
A24.1 |
op_container_end_page |
A24 |
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1766147836902113280 |