Morphological Variation among Betula nana (diploid), B. pubescens (tetraploid) and their Triploid Hybrids in Iceland

• Published in: Annals of Botany
• Main Authors: Thórsson, Æ. Th., Pálsson, S., Sigurgeirsson, A., Anamthawat-Jónsson, K.
• Format: Text
• Language: English
• Published: Oxford University Press 2007
• Subjects: INVITED REVIEW; Betula nana; Dwarf birch; Iceland
• Online Access: http://aob.oxfordjournals.org/cgi/content/short/99/6/1183; https://doi.org/10.1093/aob/mcm060

Background and Aims Introgressive hybridization between two co-existing Betula species in Iceland, diploid dwarf birch B. nana and tetraploid downy birch B. pubescens , has been well documented. The two species are highly variable morphologically, making taxonomic delineation difficult despite stable ploidy levels. Here an analysis is made of morphological variation within each ploidy group with an aim to establishing a reliable means to distinguish the species. Methods Plant materials were collected from 14 woodlands in Iceland. The plants were identified based on 2 n chromosome numbers. Morphological variation in species-specific characters within each ploidy group was analysed qualitatively and quantitatively. The morphological index was based on eight discrete characters, whereas the multivariate analysis was based on nine leaf variables. Key Results Of the 461 plants examined, 9·5 % were found to be triploid hybrids. The three ploidy groups were morphologically distinguishable but their variation overlapped. The diploid, triploid and tetraploid groups had average scores of 1·3, 4·1 and 8·3, respectively, in the morphology index scale from 0 ( B. nana ) to 13 ( B. pubescens ). A linear discriminant analysis also revealed significant separation among the three ploidy groups and the model assigned 96 % and 97 % of the B. nana and B. pubescens individuals correctly. The triploid hybrids were difficult to predict since only half of them could be assigned correctly. Leaf length was the most useful variable identifying triploid hybrids. Geographical patterns within the ploidy groups could partly be explained by differences in mean July temperature. Conclusions Hybridization between B. nana and B. pubescens is widespread in Iceland. The species can be distinguished from each other morphologically, and from the triploid hybrids. The overlapping morphological variation indicates bidirectional introgression between the two species via triploid hybrids. Iceland could be considered a birch hybrid zone, harbouring ...