Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda
Edwardsiella tarda is the pathogen responsible for edwardsiellosis, a serious infectious disease of freshwater and marine fish species, and currently recognized to be the species pathogenic for human. An anti-idiotypic monoclonal antibody (mAb), 1E11, has been developed. It mimics the protective epi...
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fthighwire:oai:open-archive.highwire.org:abbs:42/2/129 2023-05-15T18:06:08+02:00 Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda Qin, Hong Jin, Xiaohang Huang, Weiquan Liu, Yulin 2010-02-01 00:00:00.0 text/html http://abbs.oxfordjournals.org/cgi/content/short/42/2/129 https://doi.org/10.1093/abbs/gmp117 en eng Oxford University Press http://abbs.oxfordjournals.org/cgi/content/short/42/2/129 http://dx.doi.org/10.1093/abbs/gmp117 Copyright (C) 2010, Institute of Biochemistry and Cell Biology, CAS Original Articles TEXT 2010 fthighwire https://doi.org/10.1093/abbs/gmp117 2016-11-16T17:16:27Z Edwardsiella tarda is the pathogen responsible for edwardsiellosis, a serious infectious disease of freshwater and marine fish species, and currently recognized to be the species pathogenic for human. An anti-idiotypic monoclonal antibody (mAb), 1E11, has been developed. It mimics the protective epitope of E. tarda and can prevent fish from infection of E. tarda . In this study, the correct variable heavy (V H ) and variable light (V L ) genes were obtained from 1E11 by using bioinformatics methods, and a 15 amino acid (Gly 4 Ser) 3 linker was used to hold the two V domains together for the construction of V L –linker–V H form of single chain variable fragment (scFv) gene. Then, the scFv was subcloned into the vector pET-28a, expressed in the Escherichia coli BL21 cells, and identified by SDS–PAGE and western blotting. Red drum ( Sciaenops ocellatus L.) weighing about 50 g was subjected to challenge with different E. tarda strains after 4 weeks followed by vaccination, the mortality rates and relative percentage survival were recorded and calculated, and the survival rate of fish in the scFv subgroups was obviously higher than that of control subgroups ( P < 0.01). Enzyme-linked immunosorbent assay results show that after 4 weeks of post-vaccination, the level of specific antibody in fish sera of scFv groups was significantly higher than control groups. This study indicates that the recombinant antibody scFv was successfully developed, and it may serve as an effective vaccine candidate against E. tarda . Text Red drum Sciaenops ocellatus HighWire Press (Stanford University) Acta Biochimica et Biophysica Sinica 42 2 129 136 |
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Original Articles Qin, Hong Jin, Xiaohang Huang, Weiquan Liu, Yulin Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda |
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Original Articles |
description |
Edwardsiella tarda is the pathogen responsible for edwardsiellosis, a serious infectious disease of freshwater and marine fish species, and currently recognized to be the species pathogenic for human. An anti-idiotypic monoclonal antibody (mAb), 1E11, has been developed. It mimics the protective epitope of E. tarda and can prevent fish from infection of E. tarda . In this study, the correct variable heavy (V H ) and variable light (V L ) genes were obtained from 1E11 by using bioinformatics methods, and a 15 amino acid (Gly 4 Ser) 3 linker was used to hold the two V domains together for the construction of V L –linker–V H form of single chain variable fragment (scFv) gene. Then, the scFv was subcloned into the vector pET-28a, expressed in the Escherichia coli BL21 cells, and identified by SDS–PAGE and western blotting. Red drum ( Sciaenops ocellatus L.) weighing about 50 g was subjected to challenge with different E. tarda strains after 4 weeks followed by vaccination, the mortality rates and relative percentage survival were recorded and calculated, and the survival rate of fish in the scFv subgroups was obviously higher than that of control subgroups ( P < 0.01). Enzyme-linked immunosorbent assay results show that after 4 weeks of post-vaccination, the level of specific antibody in fish sera of scFv groups was significantly higher than control groups. This study indicates that the recombinant antibody scFv was successfully developed, and it may serve as an effective vaccine candidate against E. tarda . |
format |
Text |
author |
Qin, Hong Jin, Xiaohang Huang, Weiquan Liu, Yulin |
author_facet |
Qin, Hong Jin, Xiaohang Huang, Weiquan Liu, Yulin |
author_sort |
Qin, Hong |
title |
Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda |
title_short |
Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda |
title_full |
Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda |
title_fullStr |
Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda |
title_full_unstemmed |
Production of an anti-idiotypic antibody single chain variable fragment vaccine against Edwardsiella tarda |
title_sort |
production of an anti-idiotypic antibody single chain variable fragment vaccine against edwardsiella tarda |
publisher |
Oxford University Press |
publishDate |
2010 |
url |
http://abbs.oxfordjournals.org/cgi/content/short/42/2/129 https://doi.org/10.1093/abbs/gmp117 |
genre |
Red drum Sciaenops ocellatus |
genre_facet |
Red drum Sciaenops ocellatus |
op_relation |
http://abbs.oxfordjournals.org/cgi/content/short/42/2/129 http://dx.doi.org/10.1093/abbs/gmp117 |
op_rights |
Copyright (C) 2010, Institute of Biochemistry and Cell Biology, CAS |
op_doi |
https://doi.org/10.1093/abbs/gmp117 |
container_title |
Acta Biochimica et Biophysica Sinica |
container_volume |
42 |
container_issue |
2 |
container_start_page |
129 |
op_container_end_page |
136 |
_version_ |
1766177709163020288 |