Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides
Abstract The insulin-like growth factor type 1 receptor (IGF-IR) belongs to the tyrosine kinase (TK) receptor family. Besides being mitogenic, IGF-IR plays a crucial role in cell survival, transformation and maintenance of the malignant phenotype. In this study, we cloned the cDNA from the hypothala...
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fthighwire:oai:open-archive.highwire.org:abbs:37/5/327 2023-05-15T18:41:12+02:00 Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides Kuang, Yue-Mei Li, Wen-Sheng Lin, Hao-Ran 2005-05-01 00:00:00.0 text/html http://abbs.oxfordjournals.org/cgi/content/short/37/5/327 https://doi.org/10.1111/j.1745-7270.2005.00045.x en eng Oxford University Press http://abbs.oxfordjournals.org/cgi/content/short/37/5/327 http://dx.doi.org/10.1111/j.1745-7270.2005.00045.x Copyright (C) 2005, Institute of Biochemistry and Cell Biology, CAS Research Papers TEXT 2005 fthighwire https://doi.org/10.1111/j.1745-7270.2005.00045.x 2016-11-16T17:54:52Z Abstract The insulin-like growth factor type 1 receptor (IGF-IR) belongs to the tyrosine kinase (TK) receptor family. Besides being mitogenic, IGF-IR plays a crucial role in cell survival, transformation and maintenance of the malignant phenotype. In this study, we cloned the cDNA from the hypothalamus of the orange-spotted grouper ( Epinephelus coioides ) using reverse transcription PCR (RT-PCR) and the rapid amplification of cDNA ends (RACE) method. The deduced amino acid sequence showed that the receptor comprises 1413 amino acid residues. It contains cysteine-rich domains in its β-subunit, and a conserved transmembrane domain and TK domains in its β-subunit. Comparison of the amino acid sequence with those of other species showed that the grouper IGF-IR shares 90.2%, 89.6%, 71.9% and 72% similarity with the IGF-IR of the Japanese flounder, turbot, zebrafish-a and zebrafish-b, respectively. When compared with its mammalian homologue, grouper IGF-IR contains a large insertion at its C-terminus. Phylogenetic analysis has revealed that the grouper IGF-IR belongs to the b-type IGF-IRs and has a higher similarity with flounder and turbot IGF-IR, and a lower similarity (<70%) with human, mouse and avian IGF-IR. Grouper IGF-IR transcripts were detected in the brain, peripheral tissues, embryos and early development larvae by semi-quantitative RT-PCR assay. It was observed that IGF-IR mRNA expression was greater in the brain than in peripheral tissues. The level of IGF-IR mRNA expression was much higher in retina, gonad, skeletal muscle and gill tissues than in liver, heart and thymus tissues. The expression of IGF-IR can be visualized as a ubiquitous signal in unfertilized eggs, embryos and early development larvae. The distribution pattern of IGF-IR mRNA in grouper development suggests that IGF-IR plays an important role in the embryo and early larval development stages. Text Turbot HighWire Press (Stanford University) Acta Biochimica et Biophysica Sinica 37 5 327 334 |
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Research Papers Kuang, Yue-Mei Li, Wen-Sheng Lin, Hao-Ran Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
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Abstract The insulin-like growth factor type 1 receptor (IGF-IR) belongs to the tyrosine kinase (TK) receptor family. Besides being mitogenic, IGF-IR plays a crucial role in cell survival, transformation and maintenance of the malignant phenotype. In this study, we cloned the cDNA from the hypothalamus of the orange-spotted grouper ( Epinephelus coioides ) using reverse transcription PCR (RT-PCR) and the rapid amplification of cDNA ends (RACE) method. The deduced amino acid sequence showed that the receptor comprises 1413 amino acid residues. It contains cysteine-rich domains in its β-subunit, and a conserved transmembrane domain and TK domains in its β-subunit. Comparison of the amino acid sequence with those of other species showed that the grouper IGF-IR shares 90.2%, 89.6%, 71.9% and 72% similarity with the IGF-IR of the Japanese flounder, turbot, zebrafish-a and zebrafish-b, respectively. When compared with its mammalian homologue, grouper IGF-IR contains a large insertion at its C-terminus. Phylogenetic analysis has revealed that the grouper IGF-IR belongs to the b-type IGF-IRs and has a higher similarity with flounder and turbot IGF-IR, and a lower similarity (<70%) with human, mouse and avian IGF-IR. Grouper IGF-IR transcripts were detected in the brain, peripheral tissues, embryos and early development larvae by semi-quantitative RT-PCR assay. It was observed that IGF-IR mRNA expression was greater in the brain than in peripheral tissues. The level of IGF-IR mRNA expression was much higher in retina, gonad, skeletal muscle and gill tissues than in liver, heart and thymus tissues. The expression of IGF-IR can be visualized as a ubiquitous signal in unfertilized eggs, embryos and early development larvae. The distribution pattern of IGF-IR mRNA in grouper development suggests that IGF-IR plays an important role in the embryo and early larval development stages. |
format |
Text |
author |
Kuang, Yue-Mei Li, Wen-Sheng Lin, Hao-Ran |
author_facet |
Kuang, Yue-Mei Li, Wen-Sheng Lin, Hao-Ran |
author_sort |
Kuang, Yue-Mei |
title |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_short |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_full |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_fullStr |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_full_unstemmed |
Molecular Cloning and mRNA Profile of Insulin-like Growth Factor Type 1 Receptor in Orange-spotted Grouper, Epinephelus coioides |
title_sort |
molecular cloning and mrna profile of insulin-like growth factor type 1 receptor in orange-spotted grouper, epinephelus coioides |
publisher |
Oxford University Press |
publishDate |
2005 |
url |
http://abbs.oxfordjournals.org/cgi/content/short/37/5/327 https://doi.org/10.1111/j.1745-7270.2005.00045.x |
genre |
Turbot |
genre_facet |
Turbot |
op_relation |
http://abbs.oxfordjournals.org/cgi/content/short/37/5/327 http://dx.doi.org/10.1111/j.1745-7270.2005.00045.x |
op_rights |
Copyright (C) 2005, Institute of Biochemistry and Cell Biology, CAS |
op_doi |
https://doi.org/10.1111/j.1745-7270.2005.00045.x |
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Acta Biochimica et Biophysica Sinica |
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37 |
container_issue |
5 |
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327 |
op_container_end_page |
334 |
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1766230689359855616 |