In silico analysis and characterization of fresh water fish ATPases and homology modelling
ATPases is known to be a crucial in many biological activities of organisms. In this study, physicochemical properties and modeling of ATPases protein of fish was analysed using In silico approach. ATPases a protein selected from fish species, including Gold fish (Carassius auratus auratus), Zebra f...
Main Authors: | , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Annals of Proteomics and Bioinformatics - Heighten Science Publications Inc.
2017
|
Subjects: | |
Online Access: | https://www.proteobiojournal.com/articles/apb-aid1003.pdf |
id |
ftheightenscienp:oai:hspioa.org:10.29328/journal.hpbr.1001003 |
---|---|
record_format |
openpolar |
spelling |
ftheightenscienp:oai:hspioa.org:10.29328/journal.hpbr.1001003 2024-09-15T18:02:51+00:00 In silico analysis and characterization of fresh water fish ATPases and homology modelling Rumpi Ghosh AD Upadhayay AK Roy* 2017-10-11 https://www.proteobiojournal.com/articles/apb-aid1003.pdf en eng Annals of Proteomics and Bioinformatics - Heighten Science Publications Inc. https://www.proteobiojournal.com/articles/apb-aid1003.pdf Copyright et al. Research Article 2017 ftheightenscienp 2024-07-18T03:14:49Z ATPases is known to be a crucial in many biological activities of organisms. In this study, physicochemical properties and modeling of ATPases protein of fish was analysed using In silico approach. ATPases a protein selected from fish species, including Gold fish (Carassius auratus auratus), Zebra fish (Hypancistrus zebra), White fishes (Coregonus autumnalis), Grass carp (Ctenopharyngodon idella) and Anabas testudineus (Koi) were used in this study. Physicochemical characteristics showed with molecular weight (25045.58-25148.57Da), theoretical isoelectric point (9.30-9.97), extinction coefficient(26470-34950), aliphatic index(147.31-150.35), instability index(32.84-42.67), total number of negatively charged residues and positively charged residues (5/7-6/8), and grand average of hydropathicity (1.014-1.151) were computed. All proteins were classified as transmembrane proteins. In secondary structure prediction, all proteins were composed of random coils as predominant, followed by extended strands, alpha helix and beta turn. Three dimensional structure of protein were predicted and verified as good structures. All model structures were evaluated being accepted and reliable based on structural evaluation and stereo chemical analysis. Article in Journal/Newspaper Coregonus autumnalis HSPI Open Access Journals (Heighten Science Publications Inc) |
institution |
Open Polar |
collection |
HSPI Open Access Journals (Heighten Science Publications Inc) |
op_collection_id |
ftheightenscienp |
language |
English |
description |
ATPases is known to be a crucial in many biological activities of organisms. In this study, physicochemical properties and modeling of ATPases protein of fish was analysed using In silico approach. ATPases a protein selected from fish species, including Gold fish (Carassius auratus auratus), Zebra fish (Hypancistrus zebra), White fishes (Coregonus autumnalis), Grass carp (Ctenopharyngodon idella) and Anabas testudineus (Koi) were used in this study. Physicochemical characteristics showed with molecular weight (25045.58-25148.57Da), theoretical isoelectric point (9.30-9.97), extinction coefficient(26470-34950), aliphatic index(147.31-150.35), instability index(32.84-42.67), total number of negatively charged residues and positively charged residues (5/7-6/8), and grand average of hydropathicity (1.014-1.151) were computed. All proteins were classified as transmembrane proteins. In secondary structure prediction, all proteins were composed of random coils as predominant, followed by extended strands, alpha helix and beta turn. Three dimensional structure of protein were predicted and verified as good structures. All model structures were evaluated being accepted and reliable based on structural evaluation and stereo chemical analysis. |
format |
Article in Journal/Newspaper |
author |
Rumpi Ghosh AD Upadhayay AK Roy* |
spellingShingle |
Rumpi Ghosh AD Upadhayay AK Roy* In silico analysis and characterization of fresh water fish ATPases and homology modelling |
author_facet |
Rumpi Ghosh AD Upadhayay AK Roy* |
author_sort |
Rumpi Ghosh |
title |
In silico analysis and characterization of fresh water fish ATPases and homology modelling |
title_short |
In silico analysis and characterization of fresh water fish ATPases and homology modelling |
title_full |
In silico analysis and characterization of fresh water fish ATPases and homology modelling |
title_fullStr |
In silico analysis and characterization of fresh water fish ATPases and homology modelling |
title_full_unstemmed |
In silico analysis and characterization of fresh water fish ATPases and homology modelling |
title_sort |
in silico analysis and characterization of fresh water fish atpases and homology modelling |
publisher |
Annals of Proteomics and Bioinformatics - Heighten Science Publications Inc. |
publishDate |
2017 |
url |
https://www.proteobiojournal.com/articles/apb-aid1003.pdf |
genre |
Coregonus autumnalis |
genre_facet |
Coregonus autumnalis |
op_relation |
https://www.proteobiojournal.com/articles/apb-aid1003.pdf |
op_rights |
Copyright et al. |
_version_ |
1810440255841501184 |