Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία
Epileptic seizures are the clinical manifestations of structural brain abnormalities, metabolic disturbances, and inflammatory processes or genetic causes. Veterinary Proteomics is an evolving scientific field that can provide evidence regarding diagnostic and prognostic biomarkers and set therapeut...
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Format: | Doctoral or Postdoctoral Thesis |
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Aristotle University Of Thessaloniki (AUTH)
2021
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Online Access: | http://hdl.handle.net/10442/hedi/50561 https://doi.org/10.12681/eadd/50561 |
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fthedi:oai:10442/50561 2024-05-19T07:38:45+00:00 Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία Comparative proteomic analysis of the cerebrospinal fluid in dogs with recurrent epileptic seizures Baka, Ourania Μπάκα, Ουρανία 2021 http://hdl.handle.net/10442/hedi/50561 https://doi.org/10.12681/eadd/50561 gre gre Aristotle University Of Thessaloniki (AUTH) Αριστοτέλειο Πανεπιστήμιο Θεσσαλονίκης (ΑΠΘ) doi:10.12681/eadd/50561 http://hdl.handle.net/10442/hedi/50561 Πρωτεομική ανάλυση Ανοσολογική δοκιμή SPARCL Δοκιμή Western blot Ιδιοπαθής επιληψία Εγκεφαλονωτιαίο υγρό Σκύλος (πειραματόζωο) Dogs Idiopathic epilepsy SPARCL immunoassay TMT-bases proteomics Western blot Επιστήμες Υγείας Ιατρική και Επιστήμες Υγείας γενικά Health Sciences Medical and Health Sciences general PhD Thesis 2021 fthedi https://doi.org/10.12681/eadd/50561 2024-04-29T14:16:30Z Epileptic seizures are the clinical manifestations of structural brain abnormalities, metabolic disturbances, and inflammatory processes or genetic causes. Veterinary Proteomics is an evolving scientific field that can provide evidence regarding diagnostic and prognostic biomarkers and set therapeutic targets. Proteomic analysis was performed in several types of biological materials; cerebrospinal fluid (CSF) is a promising source to identify biomarkers for neurological diseases due to the direct contact with the central nervous system (CNS). This prospective study included four dog groups (group A: healthy dogs, group B: dogs with idiopathic epilepsy under antiepileptic medication (AEM), group C: dogs with idiopathic epilepsy without AEM administration, group D: dogs with structural epilepsy). The purpose of the study was to compare the proteomic profile among the four groups. Samples were analyzed by a quantitative Tandem Mass Tags labeling approach using high performance nano-liquid chromatography coupled on-line with a Q-Exactive-Plus mass-spectrometer, able to perform tandem mass spectrometric analysis (MS/MS). Identification and relative quantification were performed using Proteome Discoverer, and data were analyzed using R. Gene ontology terms were analyzed based on Canis lupus familiaris database. Data are available via ProteomeXchange with identifier PXD018893. Eighteen proteins were statistically significant among the four groups (P <0.05). Matrix metalloproteinase 2 (MMP2) and epidermal growth factor (EGF)- containing fibulin-like extracellular matrix protein 2 (EFEMP2) appeared down-regulated whereas haptoglobin (HP) and apolipoprotein A1 (APO-A1) were up-regulated (groups B, D). Tetranectin (CLEC3B) and phosphatidylethanolamine- binding protein 4 (PEBP4) were up-regulated whereas APO-A1 was down-regulated (group C). Immunoglobulin-lambda 1 (IGLL1) was down-regulated (groups B, C) and up-regulated (group D). EFEMP2 was the only protein detected among the four groups and PEBP4 was significantly ... Doctoral or Postdoctoral Thesis Canis lupus National Archive of PhD Theses (National Documentation Centre Greece) |
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Open Polar |
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National Archive of PhD Theses (National Documentation Centre Greece) |
op_collection_id |
fthedi |
language |
Greek |
topic |
Πρωτεομική ανάλυση Ανοσολογική δοκιμή SPARCL Δοκιμή Western blot Ιδιοπαθής επιληψία Εγκεφαλονωτιαίο υγρό Σκύλος (πειραματόζωο) Dogs Idiopathic epilepsy SPARCL immunoassay TMT-bases proteomics Western blot Επιστήμες Υγείας Ιατρική και Επιστήμες Υγείας γενικά Health Sciences Medical and Health Sciences general |
spellingShingle |
Πρωτεομική ανάλυση Ανοσολογική δοκιμή SPARCL Δοκιμή Western blot Ιδιοπαθής επιληψία Εγκεφαλονωτιαίο υγρό Σκύλος (πειραματόζωο) Dogs Idiopathic epilepsy SPARCL immunoassay TMT-bases proteomics Western blot Επιστήμες Υγείας Ιατρική και Επιστήμες Υγείας γενικά Health Sciences Medical and Health Sciences general Baka, Ourania Μπάκα, Ουρανία Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία |
topic_facet |
Πρωτεομική ανάλυση Ανοσολογική δοκιμή SPARCL Δοκιμή Western blot Ιδιοπαθής επιληψία Εγκεφαλονωτιαίο υγρό Σκύλος (πειραματόζωο) Dogs Idiopathic epilepsy SPARCL immunoassay TMT-bases proteomics Western blot Επιστήμες Υγείας Ιατρική και Επιστήμες Υγείας γενικά Health Sciences Medical and Health Sciences general |
description |
Epileptic seizures are the clinical manifestations of structural brain abnormalities, metabolic disturbances, and inflammatory processes or genetic causes. Veterinary Proteomics is an evolving scientific field that can provide evidence regarding diagnostic and prognostic biomarkers and set therapeutic targets. Proteomic analysis was performed in several types of biological materials; cerebrospinal fluid (CSF) is a promising source to identify biomarkers for neurological diseases due to the direct contact with the central nervous system (CNS). This prospective study included four dog groups (group A: healthy dogs, group B: dogs with idiopathic epilepsy under antiepileptic medication (AEM), group C: dogs with idiopathic epilepsy without AEM administration, group D: dogs with structural epilepsy). The purpose of the study was to compare the proteomic profile among the four groups. Samples were analyzed by a quantitative Tandem Mass Tags labeling approach using high performance nano-liquid chromatography coupled on-line with a Q-Exactive-Plus mass-spectrometer, able to perform tandem mass spectrometric analysis (MS/MS). Identification and relative quantification were performed using Proteome Discoverer, and data were analyzed using R. Gene ontology terms were analyzed based on Canis lupus familiaris database. Data are available via ProteomeXchange with identifier PXD018893. Eighteen proteins were statistically significant among the four groups (P <0.05). Matrix metalloproteinase 2 (MMP2) and epidermal growth factor (EGF)- containing fibulin-like extracellular matrix protein 2 (EFEMP2) appeared down-regulated whereas haptoglobin (HP) and apolipoprotein A1 (APO-A1) were up-regulated (groups B, D). Tetranectin (CLEC3B) and phosphatidylethanolamine- binding protein 4 (PEBP4) were up-regulated whereas APO-A1 was down-regulated (group C). Immunoglobulin-lambda 1 (IGLL1) was down-regulated (groups B, C) and up-regulated (group D). EFEMP2 was the only protein detected among the four groups and PEBP4 was significantly ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Baka, Ourania Μπάκα, Ουρανία |
author_facet |
Baka, Ourania Μπάκα, Ουρανία |
author_sort |
Baka, Ourania |
title |
Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία |
title_short |
Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία |
title_full |
Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία |
title_fullStr |
Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία |
title_full_unstemmed |
Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία |
title_sort |
συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία |
publisher |
Aristotle University Of Thessaloniki (AUTH) |
publishDate |
2021 |
url |
http://hdl.handle.net/10442/hedi/50561 https://doi.org/10.12681/eadd/50561 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_relation |
doi:10.12681/eadd/50561 http://hdl.handle.net/10442/hedi/50561 |
op_doi |
https://doi.org/10.12681/eadd/50561 |
_version_ |
1799478255564095488 |