Συγκριτική πρωτεωμική ανάλυση του εγκεφαλονωτιαίου υγρού σε σκύλους με ιδιοπαθή και με επίκτητη επιληψία

• Main Authors: Baka, Ourania, Μπάκα, Ουρανία
• Format: Doctoral or Postdoctoral Thesis
• Language: Greek
• Published: Aristotle University Of Thessaloniki (AUTH) 2021
• Subjects: Πρωτεομική ανάλυση; Ανοσολογική δοκιμή SPARCL; Δοκιμή Western blot; Ιδιοπαθής επιληψία; Εγκεφαλονωτιαίο υγρό; Σκύλος (πειραματόζωο); Dogs; Idiopathic epilepsy; SPARCL immunoassay; TMT-bases proteomics; Western blot; Επιστήμες Υγείας; Ιατρική και Επιστήμες Υγείας; γενικά; Health Sciences; Medical and Health Sciences; general; Canis lupus
• Online Access: http://hdl.handle.net/10442/hedi/50561; https://doi.org/10.12681/eadd/50561

Epileptic seizures are the clinical manifestations of structural brain abnormalities, metabolic disturbances, and inflammatory processes or genetic causes. Veterinary Proteomics is an evolving scientific field that can provide evidence regarding diagnostic and prognostic biomarkers and set therapeutic targets. Proteomic analysis was performed in several types of biological materials; cerebrospinal fluid (CSF) is a promising source to identify biomarkers for neurological diseases due to the direct contact with the central nervous system (CNS). This prospective study included four dog groups (group A: healthy dogs, group B: dogs with idiopathic epilepsy under antiepileptic medication (AEM), group C: dogs with idiopathic epilepsy without AEM administration, group D: dogs with structural epilepsy). The purpose of the study was to compare the proteomic profile among the four groups. Samples were analyzed by a quantitative Tandem Mass Tags labeling approach using high performance nano-liquid chromatography coupled on-line with a Q-Exactive-Plus mass-spectrometer, able to perform tandem mass spectrometric analysis (MS/MS). Identification and relative quantification were performed using Proteome Discoverer, and data were analyzed using R. Gene ontology terms were analyzed based on Canis lupus familiaris database. Data are available via ProteomeXchange with identifier PXD018893. Eighteen proteins were statistically significant among the four groups (P <0.05). Matrix metalloproteinase 2 (MMP2) and epidermal growth factor (EGF)- containing fibulin-like extracellular matrix protein 2 (EFEMP2) appeared down-regulated whereas haptoglobin (HP) and apolipoprotein A1 (APO-A1) were up-regulated (groups B, D). Tetranectin (CLEC3B) and phosphatidylethanolamine- binding protein 4 (PEBP4) were up-regulated whereas APO-A1 was down-regulated (group C). Immunoglobulin-lambda 1 (IGLL1) was down-regulated (groups B, C) and up-regulated (group D). EFEMP2 was the only protein detected among the four groups and PEBP4 was significantly ...