A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo salar) with Cardiomyopathy Syndrome (CMS)
Background: Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PRV), whic...
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ftharvardudash:oai:dash.harvard.edu:1/4878060 2023-05-15T15:30:08+02:00 A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo salar) with Cardiomyopathy Syndrome (CMS) Løvoll, Marie Wiik-Nielsen, Jannicke Grove, Søren Wiik-Nielsen, Christer R Kristoffersen, Anja B Faller, Randi Poppe, Trygve Jung, Joonil Pedamallu, Chandra S Nederbragt, Alexander J Rimstad, Espen Tengs, Torstein Meyerson, Matthew Langer 2010 application/pdf http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878060 https://doi.org/10.1186/1743-422X-7-309 en_US eng BioMed Central doi:10.1186/1743-422X-7-309 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994541/pdf/ Virology Journal Løvoll, Marie, Jannicke Wiik-Nielsen, Søren Grove, Christer R. Wiik-Nielsen, Anja B. Kristoffersen, Randi Faller, Trygve Poppe, et al. 2010. A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS). Virology Journal 7: 309. 1743-422X http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878060 Journal Article 2010 ftharvardudash https://doi.org/10.1186/1743-422X-7-309 2022-04-04T12:43:47Z Background: Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PRV), which is associated with another cardiac disease in Atlantic salmon; heart and skeletal muscle inflammation (HSMI). In the present work we have studied whether PRV or other infectious agents may be involved in the etiology of CMS. Results: Using high throughput sequencing on heart samples from natural outbreaks of CMS and from fish experimentally challenged with material from fish diagnosed with CMS a high number of sequence reads identical to the PRV genome were identified. In addition, a sequence contig from a novel totivirus could also be constructed. Using RT-qPCR, levels of PRV in tissue samples were quantified and the totivirus was detected in all samples tested from CMS fish but not in controls. In situ hybridization supported this pattern indicating a possible association between CMS and the novel piscine totivirus. Conclusions: Although causality for CMS in Atlantic salmon could not be proven for either of the two viruses, our results are compatible with a hypothesis where, in the experimental challenge studied, PRV behaves as an opportunist whereas the totivirus might be more directly linked with the development of CMS. Version of Record Article in Journal/Newspaper Atlantic salmon Salmo salar Harvard University: DASH - Digital Access to Scholarship at Harvard Slaughter ENVELOPE(-85.633,-85.633,-78.617,-78.617) Virology Journal 7 1 309 |
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Open Polar |
collection |
Harvard University: DASH - Digital Access to Scholarship at Harvard |
op_collection_id |
ftharvardudash |
language |
English |
description |
Background: Cardiomyopathy syndrome (CMS) is a severe disease affecting large farmed Atlantic salmon. Mortality often appears without prior clinical signs, typically shortly prior to slaughter. We recently reported the finding and the complete genomic sequence of a novel piscine reovirus (PRV), which is associated with another cardiac disease in Atlantic salmon; heart and skeletal muscle inflammation (HSMI). In the present work we have studied whether PRV or other infectious agents may be involved in the etiology of CMS. Results: Using high throughput sequencing on heart samples from natural outbreaks of CMS and from fish experimentally challenged with material from fish diagnosed with CMS a high number of sequence reads identical to the PRV genome were identified. In addition, a sequence contig from a novel totivirus could also be constructed. Using RT-qPCR, levels of PRV in tissue samples were quantified and the totivirus was detected in all samples tested from CMS fish but not in controls. In situ hybridization supported this pattern indicating a possible association between CMS and the novel piscine totivirus. Conclusions: Although causality for CMS in Atlantic salmon could not be proven for either of the two viruses, our results are compatible with a hypothesis where, in the experimental challenge studied, PRV behaves as an opportunist whereas the totivirus might be more directly linked with the development of CMS. Version of Record |
format |
Article in Journal/Newspaper |
author |
Løvoll, Marie Wiik-Nielsen, Jannicke Grove, Søren Wiik-Nielsen, Christer R Kristoffersen, Anja B Faller, Randi Poppe, Trygve Jung, Joonil Pedamallu, Chandra S Nederbragt, Alexander J Rimstad, Espen Tengs, Torstein Meyerson, Matthew Langer |
spellingShingle |
Løvoll, Marie Wiik-Nielsen, Jannicke Grove, Søren Wiik-Nielsen, Christer R Kristoffersen, Anja B Faller, Randi Poppe, Trygve Jung, Joonil Pedamallu, Chandra S Nederbragt, Alexander J Rimstad, Espen Tengs, Torstein Meyerson, Matthew Langer A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo salar) with Cardiomyopathy Syndrome (CMS) |
author_facet |
Løvoll, Marie Wiik-Nielsen, Jannicke Grove, Søren Wiik-Nielsen, Christer R Kristoffersen, Anja B Faller, Randi Poppe, Trygve Jung, Joonil Pedamallu, Chandra S Nederbragt, Alexander J Rimstad, Espen Tengs, Torstein Meyerson, Matthew Langer |
author_sort |
Løvoll, Marie |
title |
A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo salar) with Cardiomyopathy Syndrome (CMS) |
title_short |
A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo salar) with Cardiomyopathy Syndrome (CMS) |
title_full |
A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo salar) with Cardiomyopathy Syndrome (CMS) |
title_fullStr |
A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo salar) with Cardiomyopathy Syndrome (CMS) |
title_full_unstemmed |
A Novel Totivirus and Piscine Reovirus (PRV) in Atlantic Salmon (Salmo salar) with Cardiomyopathy Syndrome (CMS) |
title_sort |
novel totivirus and piscine reovirus (prv) in atlantic salmon (salmo salar) with cardiomyopathy syndrome (cms) |
publisher |
BioMed Central |
publishDate |
2010 |
url |
http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878060 https://doi.org/10.1186/1743-422X-7-309 |
long_lat |
ENVELOPE(-85.633,-85.633,-78.617,-78.617) |
geographic |
Slaughter |
geographic_facet |
Slaughter |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.1186/1743-422X-7-309 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2994541/pdf/ Virology Journal Løvoll, Marie, Jannicke Wiik-Nielsen, Søren Grove, Christer R. Wiik-Nielsen, Anja B. Kristoffersen, Randi Faller, Trygve Poppe, et al. 2010. A novel totivirus and piscine reovirus (PRV) in Atlantic salmon (Salmo salar) with cardiomyopathy syndrome (CMS). Virology Journal 7: 309. 1743-422X http://nrs.harvard.edu/urn-3:HUL.InstRepos:4878060 |
op_doi |
https://doi.org/10.1186/1743-422X-7-309 |
container_title |
Virology Journal |
container_volume |
7 |
container_issue |
1 |
container_start_page |
309 |
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1766360587215831040 |