Whole genome analysis of Flavobacterium aziz-sancarii sp. nov., isolated from Ardley Island (Antarctica), revealed a rich resistome and bioremediation potential
© 2022 Elsevier LtdDespite being one of the most isolated regions in the world, Antarctica is at risk of increased contamination with potentially toxic elements and other toxic chemicals through anthropogenic interventions. In this study, a psychrotolerant bacterium was isolated using the lake water...
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Online Access: | https://doi.org/10.1016/j.chemosphere.2022.137511 https://avesis.hacettepe.edu.tr/publication/details/0c733335-b1e9-4de4-90a1-5cb518946a59/oai |
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fthacettepeuniav:0c733335-b1e9-4de4-90a1-5cb518946a59 2023-05-15T13:56:32+02:00 Whole genome analysis of Flavobacterium aziz-sancarii sp. nov., isolated from Ardley Island (Antarctica), revealed a rich resistome and bioremediation potential Kurt-Kızıldoğan, Aslıhan OKAY, SEZER Otur, Çiğdem 2023-02-01T00:00:00Z https://doi.org/10.1016/j.chemosphere.2022.137511 https://avesis.hacettepe.edu.tr/publication/details/0c733335-b1e9-4de4-90a1-5cb518946a59/oai eng eng 0c733335-b1e9-4de4-90a1-5cb518946a59 doi:10.1016/j.chemosphere.2022.137511 https://avesis.hacettepe.edu.tr/publication/details/0c733335-b1e9-4de4-90a1-5cb518946a59/oai info:eu-repo/semantics/closedAccess info:eu-repo/semantics/article 2023 fthacettepeuniav https://doi.org/10.1016/j.chemosphere.2022.137511 2023-02-10T11:48:20Z © 2022 Elsevier LtdDespite being one of the most isolated regions in the world, Antarctica is at risk of increased contamination with potentially toxic elements and other toxic chemicals through anthropogenic interventions. In this study, a psychrotolerant bacterium was isolated using the lake water collected from Ardley Island (Antarctica), which can grow at temperatures between 4 and 30 °C and pH values between 6.0 and 9.0. The isolate, named AC, had protease, amylase, and lipase activities with no NaCl tolerance and could degrade 1–5% diesel fuel. Multilocus sequence analysis (MLSA) using 16S rRNA, gyrB, tuf, and rpoD genes resulted in 92.91–98.6% sequence similarities between the isolate AC and other Flavobacterium spp. Whole genome analysis indicated that the genome length of Flavobacterium sp. AC is 5.8 Mbp with a GC content of 34.04% and 1274 genes predicted. The strain AC branched independently from other Flavobacterium spp. in the phylogenetic and phylogenomic trees and ranked a new species named Flavobacterium aziz-sancarii. Genome mining identified several cold-inducible genes, including stress-associated genes such as cold-shock proteins, chaperones, carotenoid biosynthetic genes, or oxidative-stress response genes. In addition, virulence, gliding motility, and biofilm-related genes were determined. Its genome contains 35 and 88 open-reading frames related to potentially toxic element and antibiotic resistance, respectively. F. aziz-sancarii showed a remarkable tolerance of Cr and Ni, with minimal inhibitory concentration values of 2.88 and 2.81 mM, respectively. Pb, Cu, and Zn exposure resulted in moderate toxicity (2.14–2.41 mM), while Cd showed the highest inhibitory effect in bacterial growth (0.74 mM). Antibiotic susceptibility testing indicated multidrug-resistant phenotype in correlation to in silico prediction of antibiotic resistance genes. Overall, our results contribute to biodiversity of Antarctica and provide new insights into resistome profile of Antarctic microorganisms. Additionally, ... Article in Journal/Newspaper Antarc* Antarctic Antarctica Ardley Island Hacettepe University Research Information System Antarctic Ardley ENVELOPE(-58.953,-58.953,-62.201,-62.201) Ardley Island ENVELOPE(-58.933,-58.933,-62.213,-62.213) Chemosphere 313 137511 |
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Open Polar |
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Hacettepe University Research Information System |
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language |
English |
description |
© 2022 Elsevier LtdDespite being one of the most isolated regions in the world, Antarctica is at risk of increased contamination with potentially toxic elements and other toxic chemicals through anthropogenic interventions. In this study, a psychrotolerant bacterium was isolated using the lake water collected from Ardley Island (Antarctica), which can grow at temperatures between 4 and 30 °C and pH values between 6.0 and 9.0. The isolate, named AC, had protease, amylase, and lipase activities with no NaCl tolerance and could degrade 1–5% diesel fuel. Multilocus sequence analysis (MLSA) using 16S rRNA, gyrB, tuf, and rpoD genes resulted in 92.91–98.6% sequence similarities between the isolate AC and other Flavobacterium spp. Whole genome analysis indicated that the genome length of Flavobacterium sp. AC is 5.8 Mbp with a GC content of 34.04% and 1274 genes predicted. The strain AC branched independently from other Flavobacterium spp. in the phylogenetic and phylogenomic trees and ranked a new species named Flavobacterium aziz-sancarii. Genome mining identified several cold-inducible genes, including stress-associated genes such as cold-shock proteins, chaperones, carotenoid biosynthetic genes, or oxidative-stress response genes. In addition, virulence, gliding motility, and biofilm-related genes were determined. Its genome contains 35 and 88 open-reading frames related to potentially toxic element and antibiotic resistance, respectively. F. aziz-sancarii showed a remarkable tolerance of Cr and Ni, with minimal inhibitory concentration values of 2.88 and 2.81 mM, respectively. Pb, Cu, and Zn exposure resulted in moderate toxicity (2.14–2.41 mM), while Cd showed the highest inhibitory effect in bacterial growth (0.74 mM). Antibiotic susceptibility testing indicated multidrug-resistant phenotype in correlation to in silico prediction of antibiotic resistance genes. Overall, our results contribute to biodiversity of Antarctica and provide new insights into resistome profile of Antarctic microorganisms. Additionally, ... |
format |
Article in Journal/Newspaper |
author |
Kurt-Kızıldoğan, Aslıhan OKAY, SEZER Otur, Çiğdem |
spellingShingle |
Kurt-Kızıldoğan, Aslıhan OKAY, SEZER Otur, Çiğdem Whole genome analysis of Flavobacterium aziz-sancarii sp. nov., isolated from Ardley Island (Antarctica), revealed a rich resistome and bioremediation potential |
author_facet |
Kurt-Kızıldoğan, Aslıhan OKAY, SEZER Otur, Çiğdem |
author_sort |
Kurt-Kızıldoğan, Aslıhan |
title |
Whole genome analysis of Flavobacterium aziz-sancarii sp. nov., isolated from Ardley Island (Antarctica), revealed a rich resistome and bioremediation potential |
title_short |
Whole genome analysis of Flavobacterium aziz-sancarii sp. nov., isolated from Ardley Island (Antarctica), revealed a rich resistome and bioremediation potential |
title_full |
Whole genome analysis of Flavobacterium aziz-sancarii sp. nov., isolated from Ardley Island (Antarctica), revealed a rich resistome and bioremediation potential |
title_fullStr |
Whole genome analysis of Flavobacterium aziz-sancarii sp. nov., isolated from Ardley Island (Antarctica), revealed a rich resistome and bioremediation potential |
title_full_unstemmed |
Whole genome analysis of Flavobacterium aziz-sancarii sp. nov., isolated from Ardley Island (Antarctica), revealed a rich resistome and bioremediation potential |
title_sort |
whole genome analysis of flavobacterium aziz-sancarii sp. nov., isolated from ardley island (antarctica), revealed a rich resistome and bioremediation potential |
publishDate |
2023 |
url |
https://doi.org/10.1016/j.chemosphere.2022.137511 https://avesis.hacettepe.edu.tr/publication/details/0c733335-b1e9-4de4-90a1-5cb518946a59/oai |
long_lat |
ENVELOPE(-58.953,-58.953,-62.201,-62.201) ENVELOPE(-58.933,-58.933,-62.213,-62.213) |
geographic |
Antarctic Ardley Ardley Island |
geographic_facet |
Antarctic Ardley Ardley Island |
genre |
Antarc* Antarctic Antarctica Ardley Island |
genre_facet |
Antarc* Antarctic Antarctica Ardley Island |
op_relation |
0c733335-b1e9-4de4-90a1-5cb518946a59 doi:10.1016/j.chemosphere.2022.137511 https://avesis.hacettepe.edu.tr/publication/details/0c733335-b1e9-4de4-90a1-5cb518946a59/oai |
op_rights |
info:eu-repo/semantics/closedAccess |
op_doi |
https://doi.org/10.1016/j.chemosphere.2022.137511 |
container_title |
Chemosphere |
container_volume |
313 |
container_start_page |
137511 |
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1766264032326582272 |