A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species
This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For th...
Published in: | Potravinarstvo Slovak Journal of Food Sciences |
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Language: | English |
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HACCP Consulting
2022
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Online Access: | https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777 https://doi.org/10.5219/1777 |
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fthaccp:oai:ojs2.potravinarstvo.com:article/1777 2023-05-15T16:19:23+02:00 A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species Ali, Ashraf Di Taranto, Pietro Parisi, Antonio Del Sambro, Laura Iannacci, Antonio Belluscio, Donatella Debernardis, Daniela Paola Normanno, Giovanni 2022-10-12 application/pdf text/xml https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777 https://doi.org/10.5219/1777 eng eng HACCP Consulting https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777/2139 https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777/2143 https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777 doi:10.5219/1777 Copyright (c) 2022 Potravinarstvo Slovak Journal of Food Sciences https://creativecommons.org/licenses/by/4.0 CC-BY Potravinárstvo; Vol 16 (2022): Potravinarstvo Slovak Journal of Food Sciences; 656-669 Potravinarstvo Slovak Journal of Food Sciences; Vol. 16 (2022): Potravinarstvo Slovak Journal of Food Sciences; 656-669 1337-0960 fishery products fish frauds Multiplex PCR COI gene info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion 2022 fthaccp https://doi.org/10.5219/1777 2022-12-27T17:15:16Z This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For this study, a local market survey on fish was carried out to evaluate the application of labelling laws and to detect fraudulent actions using the developed PCR protocols. Ten specimens of each fish species of interest were obtained from wholesale fishery plants and were utilized for the protocol development. DNA was extracted from the individual samples and quantified. DNA isolates were subjected to end-point PCR and the PCR products were sequenced. For the identification of fish species, novel species-specific primers were developed by the program "Primer Express 3.0" and by the software “Primer-BLAST” to amplify fragments of 200 bp, 250 bp, 300 and 562 bp, 350 bp, 400 bp and 522 bp within the COI gene for M. merluccius, L. niloticus, G. morhua, R. pretiosus, P. hypophthalmus, Epinephelus spp., respectively. Single PCR was performed using DNA isolates and developed primers for each fish species of interest. After sequencing, the isolates were compared with the selected sequences of the COI gene and showed a similarity ranging from 99 to 100%. Among 43 samples obtained for the survey, 19 (44.2%) were mislabelled, with 18 (41.9%) mislabelled samples from local fisheries and fish marketplaces and 1 (2.32%) from hypermarket stores. Among fish samples purchased at local fisheries and fish marketplaces, fraudulent actions were observed more frequently in fish slices (100%) than fish fillets (65%). Regarding fish fillets, out of four samples labelled as grouper, three were L. niloticus and one P. hypophthalmus. Two fillets marketed as cod were substituted with L. niloticus. Five samples labelled as “fillet” and two samples labelled as “perch” were identified as P. hypophthalmus. Regarding fish slices, all samples marketed as ... Article in Journal/Newspaper Gadus morhua Potravinarstvo (E-Journal) Potravinarstvo Slovak Journal of Food Sciences 16 656 669 |
institution |
Open Polar |
collection |
Potravinarstvo (E-Journal) |
op_collection_id |
fthaccp |
language |
English |
topic |
fishery products fish frauds Multiplex PCR COI gene |
spellingShingle |
fishery products fish frauds Multiplex PCR COI gene Ali, Ashraf Di Taranto, Pietro Parisi, Antonio Del Sambro, Laura Iannacci, Antonio Belluscio, Donatella Debernardis, Daniela Paola Normanno, Giovanni A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
topic_facet |
fishery products fish frauds Multiplex PCR COI gene |
description |
This study developed a simple, specific, and affordable PCR-sequencing-COI gene-based protocol for the simultaneous identification of some important commercial fish species: Merluccius merluccius, Lates niloticus, Gadus morhua, Ruvettus pretiosus, Pangasianodon hypophthalmus, Epinephelus spp. For this study, a local market survey on fish was carried out to evaluate the application of labelling laws and to detect fraudulent actions using the developed PCR protocols. Ten specimens of each fish species of interest were obtained from wholesale fishery plants and were utilized for the protocol development. DNA was extracted from the individual samples and quantified. DNA isolates were subjected to end-point PCR and the PCR products were sequenced. For the identification of fish species, novel species-specific primers were developed by the program "Primer Express 3.0" and by the software “Primer-BLAST” to amplify fragments of 200 bp, 250 bp, 300 and 562 bp, 350 bp, 400 bp and 522 bp within the COI gene for M. merluccius, L. niloticus, G. morhua, R. pretiosus, P. hypophthalmus, Epinephelus spp., respectively. Single PCR was performed using DNA isolates and developed primers for each fish species of interest. After sequencing, the isolates were compared with the selected sequences of the COI gene and showed a similarity ranging from 99 to 100%. Among 43 samples obtained for the survey, 19 (44.2%) were mislabelled, with 18 (41.9%) mislabelled samples from local fisheries and fish marketplaces and 1 (2.32%) from hypermarket stores. Among fish samples purchased at local fisheries and fish marketplaces, fraudulent actions were observed more frequently in fish slices (100%) than fish fillets (65%). Regarding fish fillets, out of four samples labelled as grouper, three were L. niloticus and one P. hypophthalmus. Two fillets marketed as cod were substituted with L. niloticus. Five samples labelled as “fillet” and two samples labelled as “perch” were identified as P. hypophthalmus. Regarding fish slices, all samples marketed as ... |
format |
Article in Journal/Newspaper |
author |
Ali, Ashraf Di Taranto, Pietro Parisi, Antonio Del Sambro, Laura Iannacci, Antonio Belluscio, Donatella Debernardis, Daniela Paola Normanno, Giovanni |
author_facet |
Ali, Ashraf Di Taranto, Pietro Parisi, Antonio Del Sambro, Laura Iannacci, Antonio Belluscio, Donatella Debernardis, Daniela Paola Normanno, Giovanni |
author_sort |
Ali, Ashraf |
title |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_short |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_full |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_fullStr |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_full_unstemmed |
A fish market survey using a novel PCR-sequencing-based protocols for the identification of commercial significant fish species |
title_sort |
fish market survey using a novel pcr-sequencing-based protocols for the identification of commercial significant fish species |
publisher |
HACCP Consulting |
publishDate |
2022 |
url |
https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777 https://doi.org/10.5219/1777 |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Potravinárstvo; Vol 16 (2022): Potravinarstvo Slovak Journal of Food Sciences; 656-669 Potravinarstvo Slovak Journal of Food Sciences; Vol. 16 (2022): Potravinarstvo Slovak Journal of Food Sciences; 656-669 1337-0960 |
op_relation |
https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777/2139 https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777/2143 https://potravinarstvo.com/journal1/index.php/potravinarstvo/article/view/1777 doi:10.5219/1777 |
op_rights |
Copyright (c) 2022 Potravinarstvo Slovak Journal of Food Sciences https://creativecommons.org/licenses/by/4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.5219/1777 |
container_title |
Potravinarstvo Slovak Journal of Food Sciences |
container_volume |
16 |
container_start_page |
656 |
op_container_end_page |
669 |
_version_ |
1766005769191292928 |