Sexing Pinnipeds with ZFX and ZFY Loci
We developed and tested a protocol for determining the sex of individual pinnipeds using the sex-chromosome-specific genes ZFX and ZFY. We screened a total of 368 seals (168 crabeater, Lobodon carcinophaga; 159 Weddell, Leptonychotes weddellii; and 41 Ross, Ommatophoca rossii) of known or unknown se...
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Oxford University Press
2007
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Online Access: | http://hdl.handle.net/10072/59205 https://doi.org/10.1093/jhered/esm023 |
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ftgriffithuniv:oai:research-repository.griffith.edu.au:10072/59205 2023-05-15T13:37:02+02:00 Sexing Pinnipeds with ZFX and ZFY Loci Curtis, Caitlin S. Stewart, Brent A. Karl, Stephen 2007 http://hdl.handle.net/10072/59205 https://doi.org/10.1093/jhered/esm023 English eng Oxford University Press Journal of Heredity Population Ecological and Evolutionary Genetics Journal article 2007 ftgriffithuniv https://doi.org/10.1093/jhered/esm023 2018-07-30T10:50:34Z We developed and tested a protocol for determining the sex of individual pinnipeds using the sex-chromosome-specific genes ZFX and ZFY. We screened a total of 368 seals (168 crabeater, Lobodon carcinophaga; 159 Weddell, Leptonychotes weddellii; and 41 Ross, Ommatophoca rossii) of known or unknown sex and compared the molecular sex to the sex assigned at the time of biopsy sample collection in the Ross and Amundsen seas, Antarctica. We also screened 6 captive northern elephant seals (Mirounga angustirostris) and 2 captive California sea lions (Zalophus californianus) of known sex. The assigned sex and genetic sex agreed for virtually all seals. Indeed, discrepancies ranged from 0.0% to 6.7% among species. It is not clear, however, if the few mis-assignments of sex occurred in situ or in the laboratory. The assigned morphological and molecular sex might both be correct with the discrepancies owing perhaps to developmental effects of environmental pollution. A subset of individuals sequenced at both loci revealed no intraspecific sequence variation. There was, however, sequence variation among species at both loci, which allowed them to be uniquely identified with as few as 2 and as many as 31 nucleotides. No Full Text Article in Journal/Newspaper Antarc* Antarctica Elephant Seals Griffith University: Griffith Research Online Weddell Journal of Heredity 98 3 280 285 |
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Open Polar |
collection |
Griffith University: Griffith Research Online |
op_collection_id |
ftgriffithuniv |
language |
English |
topic |
Population Ecological and Evolutionary Genetics |
spellingShingle |
Population Ecological and Evolutionary Genetics Curtis, Caitlin S. Stewart, Brent A. Karl, Stephen Sexing Pinnipeds with ZFX and ZFY Loci |
topic_facet |
Population Ecological and Evolutionary Genetics |
description |
We developed and tested a protocol for determining the sex of individual pinnipeds using the sex-chromosome-specific genes ZFX and ZFY. We screened a total of 368 seals (168 crabeater, Lobodon carcinophaga; 159 Weddell, Leptonychotes weddellii; and 41 Ross, Ommatophoca rossii) of known or unknown sex and compared the molecular sex to the sex assigned at the time of biopsy sample collection in the Ross and Amundsen seas, Antarctica. We also screened 6 captive northern elephant seals (Mirounga angustirostris) and 2 captive California sea lions (Zalophus californianus) of known sex. The assigned sex and genetic sex agreed for virtually all seals. Indeed, discrepancies ranged from 0.0% to 6.7% among species. It is not clear, however, if the few mis-assignments of sex occurred in situ or in the laboratory. The assigned morphological and molecular sex might both be correct with the discrepancies owing perhaps to developmental effects of environmental pollution. A subset of individuals sequenced at both loci revealed no intraspecific sequence variation. There was, however, sequence variation among species at both loci, which allowed them to be uniquely identified with as few as 2 and as many as 31 nucleotides. No Full Text |
format |
Article in Journal/Newspaper |
author |
Curtis, Caitlin S. Stewart, Brent A. Karl, Stephen |
author_facet |
Curtis, Caitlin S. Stewart, Brent A. Karl, Stephen |
author_sort |
Curtis, Caitlin |
title |
Sexing Pinnipeds with ZFX and ZFY Loci |
title_short |
Sexing Pinnipeds with ZFX and ZFY Loci |
title_full |
Sexing Pinnipeds with ZFX and ZFY Loci |
title_fullStr |
Sexing Pinnipeds with ZFX and ZFY Loci |
title_full_unstemmed |
Sexing Pinnipeds with ZFX and ZFY Loci |
title_sort |
sexing pinnipeds with zfx and zfy loci |
publisher |
Oxford University Press |
publishDate |
2007 |
url |
http://hdl.handle.net/10072/59205 https://doi.org/10.1093/jhered/esm023 |
geographic |
Weddell |
geographic_facet |
Weddell |
genre |
Antarc* Antarctica Elephant Seals |
genre_facet |
Antarc* Antarctica Elephant Seals |
op_relation |
Journal of Heredity |
op_doi |
https://doi.org/10.1093/jhered/esm023 |
container_title |
Journal of Heredity |
container_volume |
98 |
container_issue |
3 |
container_start_page |
280 |
op_container_end_page |
285 |
_version_ |
1766087231304368128 |