Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions
Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action i...
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ftgriffithuniv:oai:research-repository.griffith.edu.au:10072/393155 2024-06-23T07:53:35+00:00 Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions Maner, Jenny Burkard, Michael Cassano, Juan Carlos Nash, Susan M Bengtson Schirmer, Kristin Suter, Marc J-F 2019 http://hdl.handle.net/10072/393155 https://doi.org/10.1039/c9ra05352b English eng eng Royal Society of Chemistry RSC Advances Maner, J; Burkard, M; Cassano, JC; Nash, SMB; Schirmer, K; Suter, MJ-F, Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions, RSC Advances, 2019, 9 (67), pp. 39447-39457 http://hdl.handle.net/10072/393155 2046-2069 doi:10.1039/c9ra05352b http://creativecommons.org/licenses/by-nc/3.0/ © The Author(s) 2019. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited. open access Chemical sciences Science & Technology Physical Sciences Chemistry Multidisciplinary PERSISTENT ORGANIC POLLUTANTS Journal article 2019 ftgriffithuniv https://doi.org/10.1039/c9ra05352b 2024-06-12T00:12:57Z Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action is a matter of contention. Potential toxicological effects to humpback whales remain entirely unknown. The recently established humpback whale fibroblast cell line (HuWa) offers an in vitro model for toxicological investigations. We here combine this novel cell line with a passive dosing strategy to investigate whale-specific toxicity of HCB. The relevant partitioning coefficients were determined to produce stable and predictable exposure concentrations in small-scale bioassays. The system was used to assess acute toxicity as well as genotoxicity of HCB to the HuWa cell line. While we found some transient reductions in metabolic activity, measured with the indicator dye alamarBlue, no clear acute toxic effects were discernible. Yet, a significant increase in DNA damage, detected in the alkaline comet assay, was found in HuWa cells exposed to 10 μg L−1 HCB during the sensitive phase of cell attachment. Collectively, this work provides a ready-to-use passive dosing system and delivers evidence that HCB elicits genotoxicity in humpback whale cells. Full Text Article in Journal/Newspaper Humpback Whale Griffith University: Griffith Research Online RSC Advances 9 67 39447 39457 |
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Open Polar |
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Griffith University: Griffith Research Online |
op_collection_id |
ftgriffithuniv |
language |
English |
topic |
Chemical sciences Science & Technology Physical Sciences Chemistry Multidisciplinary PERSISTENT ORGANIC POLLUTANTS |
spellingShingle |
Chemical sciences Science & Technology Physical Sciences Chemistry Multidisciplinary PERSISTENT ORGANIC POLLUTANTS Maner, Jenny Burkard, Michael Cassano, Juan Carlos Nash, Susan M Bengtson Schirmer, Kristin Suter, Marc J-F Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
topic_facet |
Chemical sciences Science & Technology Physical Sciences Chemistry Multidisciplinary PERSISTENT ORGANIC POLLUTANTS |
description |
Humpback whales, like other polar wildlife, accumulate persistent organic pollutants. In Southern hemisphere populations, hexachlorobenzene (HCB) dominates the contaminant profiles. HCB is linked to a variety of health effects and is classified as a group 2B carcinogen, but the mechanism of action is a matter of contention. Potential toxicological effects to humpback whales remain entirely unknown. The recently established humpback whale fibroblast cell line (HuWa) offers an in vitro model for toxicological investigations. We here combine this novel cell line with a passive dosing strategy to investigate whale-specific toxicity of HCB. The relevant partitioning coefficients were determined to produce stable and predictable exposure concentrations in small-scale bioassays. The system was used to assess acute toxicity as well as genotoxicity of HCB to the HuWa cell line. While we found some transient reductions in metabolic activity, measured with the indicator dye alamarBlue, no clear acute toxic effects were discernible. Yet, a significant increase in DNA damage, detected in the alkaline comet assay, was found in HuWa cells exposed to 10 μg L−1 HCB during the sensitive phase of cell attachment. Collectively, this work provides a ready-to-use passive dosing system and delivers evidence that HCB elicits genotoxicity in humpback whale cells. Full Text |
format |
Article in Journal/Newspaper |
author |
Maner, Jenny Burkard, Michael Cassano, Juan Carlos Nash, Susan M Bengtson Schirmer, Kristin Suter, Marc J-F |
author_facet |
Maner, Jenny Burkard, Michael Cassano, Juan Carlos Nash, Susan M Bengtson Schirmer, Kristin Suter, Marc J-F |
author_sort |
Maner, Jenny |
title |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_short |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_full |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_fullStr |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_full_unstemmed |
Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
title_sort |
hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions |
publisher |
Royal Society of Chemistry |
publishDate |
2019 |
url |
http://hdl.handle.net/10072/393155 https://doi.org/10.1039/c9ra05352b |
genre |
Humpback Whale |
genre_facet |
Humpback Whale |
op_relation |
RSC Advances Maner, J; Burkard, M; Cassano, JC; Nash, SMB; Schirmer, K; Suter, MJ-F, Hexachlorobenzene exerts genotoxic effects in a humpback whale cell line under stable exposure conditions, RSC Advances, 2019, 9 (67), pp. 39447-39457 http://hdl.handle.net/10072/393155 2046-2069 doi:10.1039/c9ra05352b |
op_rights |
http://creativecommons.org/licenses/by-nc/3.0/ © The Author(s) 2019. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 Unported (CC BY-NC 3.0) License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, providing that the work is properly cited. open access |
op_doi |
https://doi.org/10.1039/c9ra05352b |
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RSC Advances |
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9 |
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67 |
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39447 |
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1802645314034728960 |