Suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae
Coralline algae play a central role in reef ecology and have been identified as one of the most sensitive calcifying organisms to ocean acidification, and as such are potential indicators for evaluating the future effects of climate change. Despite their importance, little information is available o...
Published in: | Journal of Experimental Marine Biology and Ecology |
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Online Access: | http://hdl.handle.net/10072/340675 https://doi.org/10.1016/j.jembe.2017.02.004 |
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ftgriffithuniv:oai:research-repository.griffith.edu.au:10072/340675 2024-06-23T07:55:54+00:00 Suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae Lewis, Bonnie Diaz-Pulido, Guillermo 2017 http://hdl.handle.net/10072/340675 https://doi.org/10.1016/j.jembe.2017.02.004 English eng eng Elsevier Science Journal of Experimental Marine Biology and Ecology http://hdl.handle.net/10072/340675 0022-0981 doi:10.1016/j.jembe.2017.02.004 Environmental sciences Other environmental sciences not elsewhere classified Biological sciences Agricultural veterinary and food sciences Journal article 2017 ftgriffithuniv https://doi.org/10.1016/j.jembe.2017.02.004 2024-06-04T23:57:53Z Coralline algae play a central role in reef ecology and have been identified as one of the most sensitive calcifying organisms to ocean acidification, and as such are potential indicators for evaluating the future effects of climate change. Despite their importance, little information is available on their growth and calcification rates in situ, highlighting the need for a more effective technique to obtain these measurements. In this study we compared two alternative fluorochrome markers (calcein and calcofluor white - CFW) against the commonly used alizarin red stain in order to establish a more efficient and effective staining protocol for use in the field on both branching (Lithophyllum pygmaeum) and encrusting (Porolithon onkodes) coralline species. Each marker was tested using three different concentration and immersion times for toxicity, visibility and efficiency. Results from this study found that while alizarin displayed a high visibility frequency it also reduced growth in the branching species. The CFW staining proved unreliable for the encrusting samples with usable marks in only 3 of 9 treatments and also reduced growth in branching treatments. Calcein, however, proved to be an effective and efficient marker for in situ coralline studies with a high visibility frequency, no toxic effects on growth and a short immersion time of 3 h or less. This identification of a more efficient and effective stain to use in situ on coralline algae can potentially improve demographic studies in both the field and laboratory environments, further facilitating future climate change research. Griffith Sciences, Griffith School of Environment No Full Text Article in Journal/Newspaper Ocean acidification Griffith University: Griffith Research Online Griffith ENVELOPE(-155.500,-155.500,-85.883,-85.883) Journal of Experimental Marine Biology and Ecology 490 64 73 |
institution |
Open Polar |
collection |
Griffith University: Griffith Research Online |
op_collection_id |
ftgriffithuniv |
language |
English |
topic |
Environmental sciences Other environmental sciences not elsewhere classified Biological sciences Agricultural veterinary and food sciences |
spellingShingle |
Environmental sciences Other environmental sciences not elsewhere classified Biological sciences Agricultural veterinary and food sciences Lewis, Bonnie Diaz-Pulido, Guillermo Suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae |
topic_facet |
Environmental sciences Other environmental sciences not elsewhere classified Biological sciences Agricultural veterinary and food sciences |
description |
Coralline algae play a central role in reef ecology and have been identified as one of the most sensitive calcifying organisms to ocean acidification, and as such are potential indicators for evaluating the future effects of climate change. Despite their importance, little information is available on their growth and calcification rates in situ, highlighting the need for a more effective technique to obtain these measurements. In this study we compared two alternative fluorochrome markers (calcein and calcofluor white - CFW) against the commonly used alizarin red stain in order to establish a more efficient and effective staining protocol for use in the field on both branching (Lithophyllum pygmaeum) and encrusting (Porolithon onkodes) coralline species. Each marker was tested using three different concentration and immersion times for toxicity, visibility and efficiency. Results from this study found that while alizarin displayed a high visibility frequency it also reduced growth in the branching species. The CFW staining proved unreliable for the encrusting samples with usable marks in only 3 of 9 treatments and also reduced growth in branching treatments. Calcein, however, proved to be an effective and efficient marker for in situ coralline studies with a high visibility frequency, no toxic effects on growth and a short immersion time of 3 h or less. This identification of a more efficient and effective stain to use in situ on coralline algae can potentially improve demographic studies in both the field and laboratory environments, further facilitating future climate change research. Griffith Sciences, Griffith School of Environment No Full Text |
format |
Article in Journal/Newspaper |
author |
Lewis, Bonnie Diaz-Pulido, Guillermo |
author_facet |
Lewis, Bonnie Diaz-Pulido, Guillermo |
author_sort |
Lewis, Bonnie |
title |
Suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae |
title_short |
Suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae |
title_full |
Suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae |
title_fullStr |
Suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae |
title_full_unstemmed |
Suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae |
title_sort |
suitability of three fluorochrome markers for obtaining in situ growth rates of coralline algae |
publisher |
Elsevier Science |
publishDate |
2017 |
url |
http://hdl.handle.net/10072/340675 https://doi.org/10.1016/j.jembe.2017.02.004 |
long_lat |
ENVELOPE(-155.500,-155.500,-85.883,-85.883) |
geographic |
Griffith |
geographic_facet |
Griffith |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_relation |
Journal of Experimental Marine Biology and Ecology http://hdl.handle.net/10072/340675 0022-0981 doi:10.1016/j.jembe.2017.02.004 |
op_doi |
https://doi.org/10.1016/j.jembe.2017.02.004 |
container_title |
Journal of Experimental Marine Biology and Ecology |
container_volume |
490 |
container_start_page |
64 |
op_container_end_page |
73 |
_version_ |
1802648689419747328 |