Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds
Our ability to DNA barcode the birds of the world is based on the effective amplification and sequencing of a 648 base pair (bp) region of the mitochondrial cytochrome c oxidase (COI or cox1) gene. For many geographic regions the large numbers of vouchered specimens necessary for the construction of...
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ftgriffithuniv:oai:research-repository.griffith.edu.au:10072/32180 2023-05-15T13:51:20+02:00 Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds Patel, Selina Waugh, John D.Millar, Craig Lambert, David 2010 application/pdf http://hdl.handle.net/10072/32180 https://doi.org/10.1111/j.1755-0998.2009.02793.x English en_AU eng Wiley-Blackwell Publishing Inc Molecular Ecology Resources © 2010 Blackwell Publishing. This is the pre-peer reviewed version of the following article: Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds, Molecular Ecology Resources Volume 10, Issue 3, 2010, 431-438, which has been published in final form at 10.1111/j.1755-0998.2009.02793.x. Animal Systematics and Taxonomy Journal article 2010 ftgriffithuniv https://doi.org/10.1111/j.1755-0998.2009.02793.x 2018-07-30T10:41:56Z Our ability to DNA barcode the birds of the world is based on the effective amplification and sequencing of a 648 base pair (bp) region of the mitochondrial cytochrome c oxidase (COI or cox1) gene. For many geographic regions the large numbers of vouchered specimens necessary for the construction of a DNA barcoding database have already been collected and are available in museums and other institutions. However, many of these specimens are old (>20 years) and are stored as either fixed study skins or dried skeletons. DNA extracted from such historical samples is typically degraded and, generally, only short DNA fragments can be recovered from such specimens making the recovery of the barcoding region as a single fragment difficult. We report two sets of conserved primers that allow the amplification of the entire DNA barcoding region in either three or five overlapping fragments. These primer sets allow the recovery of DNA barcodes from valuable historical specimens that in many cases are unique in that they are unable or unlikely to be collected again. We also report three new primers that in combination allow the effective amplification from modern samples of the entire DNA barcoding region as a single DNA fragment for 17 orders of Southern Hemisphere birds. Full Text Article in Journal/Newspaper Antarc* Antarctic Griffith University: Griffith Research Online Antarctic New Zealand Molecular Ecology Resources 10 3 431 438 |
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Open Polar |
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Griffith University: Griffith Research Online |
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ftgriffithuniv |
language |
English |
topic |
Animal Systematics and Taxonomy |
spellingShingle |
Animal Systematics and Taxonomy Patel, Selina Waugh, John D.Millar, Craig Lambert, David Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
topic_facet |
Animal Systematics and Taxonomy |
description |
Our ability to DNA barcode the birds of the world is based on the effective amplification and sequencing of a 648 base pair (bp) region of the mitochondrial cytochrome c oxidase (COI or cox1) gene. For many geographic regions the large numbers of vouchered specimens necessary for the construction of a DNA barcoding database have already been collected and are available in museums and other institutions. However, many of these specimens are old (>20 years) and are stored as either fixed study skins or dried skeletons. DNA extracted from such historical samples is typically degraded and, generally, only short DNA fragments can be recovered from such specimens making the recovery of the barcoding region as a single fragment difficult. We report two sets of conserved primers that allow the amplification of the entire DNA barcoding region in either three or five overlapping fragments. These primer sets allow the recovery of DNA barcodes from valuable historical specimens that in many cases are unique in that they are unable or unlikely to be collected again. We also report three new primers that in combination allow the effective amplification from modern samples of the entire DNA barcoding region as a single DNA fragment for 17 orders of Southern Hemisphere birds. Full Text |
format |
Article in Journal/Newspaper |
author |
Patel, Selina Waugh, John D.Millar, Craig Lambert, David |
author_facet |
Patel, Selina Waugh, John D.Millar, Craig Lambert, David |
author_sort |
Patel, Selina |
title |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_short |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_full |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_fullStr |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_full_unstemmed |
Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds |
title_sort |
conserved primers for dna barcoding historical and modern samples from new zealand and antarctic birds |
publisher |
Wiley-Blackwell Publishing Inc |
publishDate |
2010 |
url |
http://hdl.handle.net/10072/32180 https://doi.org/10.1111/j.1755-0998.2009.02793.x |
geographic |
Antarctic New Zealand |
geographic_facet |
Antarctic New Zealand |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_relation |
Molecular Ecology Resources |
op_rights |
© 2010 Blackwell Publishing. This is the pre-peer reviewed version of the following article: Conserved primers for DNA barcoding historical and modern samples from New Zealand and Antarctic birds, Molecular Ecology Resources Volume 10, Issue 3, 2010, 431-438, which has been published in final form at 10.1111/j.1755-0998.2009.02793.x. |
op_doi |
https://doi.org/10.1111/j.1755-0998.2009.02793.x |
container_title |
Molecular Ecology Resources |
container_volume |
10 |
container_issue |
3 |
container_start_page |
431 |
op_container_end_page |
438 |
_version_ |
1766255154041978880 |