Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine
Bacillus fumarioli was originally isolated from geothermal soils in continental and maritime Antarctica, and recently, it has been shown to be a frequent contaminant of gelatine extracts obtained from European and American production plants. These habitats are geographically widely separated, share...
Published in: | Research in Microbiology |
---|---|
Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2004
|
Subjects: | |
Online Access: | https://researchonline.gcu.ac.uk/en/publications/90ed3e36-55c4-4d5a-8eca-2aba61c91142 https://doi.org/10.1016/j.resmic.2004.02.007 |
id |
ftglasgowcucris:oai:researchonline.gcu.ac.uk:publications/90ed3e36-55c4-4d5a-8eca-2aba61c91142 |
---|---|
record_format |
openpolar |
spelling |
ftglasgowcucris:oai:researchonline.gcu.ac.uk:publications/90ed3e36-55c4-4d5a-8eca-2aba61c91142 2024-09-15T17:45:28+00:00 Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine De Clerck, Elke Gevers, Dirk Sergeant, Kjell Rodriguez-Diaz, Marina Herman, Lieve Logan, Niall A. Van Beeumen, Jozef De Vos, Paul 2004-07-01 https://researchonline.gcu.ac.uk/en/publications/90ed3e36-55c4-4d5a-8eca-2aba61c91142 https://doi.org/10.1016/j.resmic.2004.02.007 eng eng https://researchonline.gcu.ac.uk/en/publications/90ed3e36-55c4-4d5a-8eca-2aba61c91142 info:eu-repo/semantics/restrictedAccess De Clerck , E , Gevers , D , Sergeant , K , Rodriguez-Diaz , M , Herman , L , Logan , N A , Van Beeumen , J & De Vos , P 2004 , ' Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine ' , Research in Microbiology . https://doi.org/10.1016/j.resmic.2004.02.007 Antarctica phenotype genotype Bacillus fumarioli article 2004 ftglasgowcucris https://doi.org/10.1016/j.resmic.2004.02.007 2024-08-21T23:33:47Z Bacillus fumarioli was originally isolated from geothermal soils in continental and maritime Antarctica, and recently, it has been shown to be a frequent contaminant of gelatine extracts obtained from European and American production plants. These habitats are geographically widely separated, share similar temperature and pH conditions, but have substantially different organic loads. Because of the prevalence in gelatine extracts and the dissimilarity of this habitat to geothermal soil, a comparative study was performed to assess the diversity among B. fumarioli strains and reveal possible intraspecies differences that might correspond to their niches of origin. Genomic (rep-PCR, 16S rDNA sequencing, DNA–DNA hybridisations) and phenotypic techniques (analysis of fatty acid content, total cellular proteins, metabolic and morphological traits) illustrate the very close relationship between isolates from the two niches. An abundant protein band was demonstrated for gelatine isolates only. This band was shown to result from a protein with high similarity to a stress response protein. Furthermore, subtractive hybridisation revealed genomic differences between Antarctic and gelatine isolates that may indicate adaptive evolution to a specific environment. Article in Journal/Newspaper Antarc* Antarctic Antarctica Glasgow Caledonian University (GCU): ResearchOnline Research in Microbiology 155 6 483 490 |
institution |
Open Polar |
collection |
Glasgow Caledonian University (GCU): ResearchOnline |
op_collection_id |
ftglasgowcucris |
language |
English |
topic |
Antarctica phenotype genotype Bacillus fumarioli |
spellingShingle |
Antarctica phenotype genotype Bacillus fumarioli De Clerck, Elke Gevers, Dirk Sergeant, Kjell Rodriguez-Diaz, Marina Herman, Lieve Logan, Niall A. Van Beeumen, Jozef De Vos, Paul Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine |
topic_facet |
Antarctica phenotype genotype Bacillus fumarioli |
description |
Bacillus fumarioli was originally isolated from geothermal soils in continental and maritime Antarctica, and recently, it has been shown to be a frequent contaminant of gelatine extracts obtained from European and American production plants. These habitats are geographically widely separated, share similar temperature and pH conditions, but have substantially different organic loads. Because of the prevalence in gelatine extracts and the dissimilarity of this habitat to geothermal soil, a comparative study was performed to assess the diversity among B. fumarioli strains and reveal possible intraspecies differences that might correspond to their niches of origin. Genomic (rep-PCR, 16S rDNA sequencing, DNA–DNA hybridisations) and phenotypic techniques (analysis of fatty acid content, total cellular proteins, metabolic and morphological traits) illustrate the very close relationship between isolates from the two niches. An abundant protein band was demonstrated for gelatine isolates only. This band was shown to result from a protein with high similarity to a stress response protein. Furthermore, subtractive hybridisation revealed genomic differences between Antarctic and gelatine isolates that may indicate adaptive evolution to a specific environment. |
format |
Article in Journal/Newspaper |
author |
De Clerck, Elke Gevers, Dirk Sergeant, Kjell Rodriguez-Diaz, Marina Herman, Lieve Logan, Niall A. Van Beeumen, Jozef De Vos, Paul |
author_facet |
De Clerck, Elke Gevers, Dirk Sergeant, Kjell Rodriguez-Diaz, Marina Herman, Lieve Logan, Niall A. Van Beeumen, Jozef De Vos, Paul |
author_sort |
De Clerck, Elke |
title |
Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine |
title_short |
Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine |
title_full |
Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine |
title_fullStr |
Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine |
title_full_unstemmed |
Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine |
title_sort |
genotypic and phenotypic comparison of bacillus fumarioli isolates from geothermal antarctic soil and gelatine |
publishDate |
2004 |
url |
https://researchonline.gcu.ac.uk/en/publications/90ed3e36-55c4-4d5a-8eca-2aba61c91142 https://doi.org/10.1016/j.resmic.2004.02.007 |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
De Clerck , E , Gevers , D , Sergeant , K , Rodriguez-Diaz , M , Herman , L , Logan , N A , Van Beeumen , J & De Vos , P 2004 , ' Genotypic and phenotypic comparison of Bacillus fumarioli isolates from geothermal Antarctic soil and gelatine ' , Research in Microbiology . https://doi.org/10.1016/j.resmic.2004.02.007 |
op_relation |
https://researchonline.gcu.ac.uk/en/publications/90ed3e36-55c4-4d5a-8eca-2aba61c91142 |
op_rights |
info:eu-repo/semantics/restrictedAccess |
op_doi |
https://doi.org/10.1016/j.resmic.2004.02.007 |
container_title |
Research in Microbiology |
container_volume |
155 |
container_issue |
6 |
container_start_page |
483 |
op_container_end_page |
490 |
_version_ |
1810493304670781440 |