Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches
The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approach...
Published in: | FEMS Microbiology Ecology |
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2023
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Online Access: | https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867_1/component/file_5022935/5022867.pdf |
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ftgfzpotsdam:oai:gfzpublic.gfz-potsdam.de:item_5022867 2023-11-12T04:17:50+01:00 Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches Jaarsma, A. Sipes, K. Zervas , A. Campuzano Jiménez, F. Ellegaard-Jensen, L. Thøgersen, M. Stougaard, P. Benning, L. Tranter, M. Anesio, A. 2023 application/pdf https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867_1/component/file_5022935/5022867.pdf unknown info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiad119 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867_1/component/file_5022935/5022867.pdf info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ FEMS Microbiology Ecology info:eu-repo/semantics/article 2023 ftgfzpotsdam https://doi.org/10.1093/femsec/fiad119 2023-10-30T00:44:24Z The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting. Article in Journal/Newspaper Greenland Ice Sheet GFZpublic (German Research Centre for Geosciences, Helmholtz-Zentrum Potsdam) FEMS Microbiology Ecology 99 11 |
institution |
Open Polar |
collection |
GFZpublic (German Research Centre for Geosciences, Helmholtz-Zentrum Potsdam) |
op_collection_id |
ftgfzpotsdam |
language |
unknown |
description |
The microbiome of Greenland Ice Sheet supraglacial habitats is still underinvestigated, and as a result there is a lack of representative genomes from these environments. In this study, we investigated the supraglacial microbiome through a combination of culturing-dependent and -independent approaches. We explored ice, cryoconite, biofilm, and snow biodiversity to answer: (1) how microbial diversity differs between supraglacial habitats, (2) if obtained bacterial genomes reflect dominant community members, and (3) how culturing versus high throughput sequencing changes our observations of microbial diversity in supraglacial habitats. Genomes acquired through metagenomic sequencing (133 high-quality MAGs) and whole genome sequencing (73 bacterial isolates) were compared to the metagenome assemblies to investigate abundance within the total environmental DNA. Isolates obtained in this study were not dominant taxa in the habitat they were sampled from, in contrast to the obtained MAGs. We demonstrate here the advantages of using metagenome SSU rRNA genes to reflect whole-community diversity. Additionally, we demonstrate a proof-of-concept of the application of in situ culturing in a supraglacial setting. |
format |
Article in Journal/Newspaper |
author |
Jaarsma, A. Sipes, K. Zervas , A. Campuzano Jiménez, F. Ellegaard-Jensen, L. Thøgersen, M. Stougaard, P. Benning, L. Tranter, M. Anesio, A. |
spellingShingle |
Jaarsma, A. Sipes, K. Zervas , A. Campuzano Jiménez, F. Ellegaard-Jensen, L. Thøgersen, M. Stougaard, P. Benning, L. Tranter, M. Anesio, A. Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
author_facet |
Jaarsma, A. Sipes, K. Zervas , A. Campuzano Jiménez, F. Ellegaard-Jensen, L. Thøgersen, M. Stougaard, P. Benning, L. Tranter, M. Anesio, A. |
author_sort |
Jaarsma, A. |
title |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_short |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_full |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_fullStr |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_full_unstemmed |
Exploring microbial diversity in Greenland Ice Sheet supraglacial habitats through culturing-dependent and -independent approaches |
title_sort |
exploring microbial diversity in greenland ice sheet supraglacial habitats through culturing-dependent and -independent approaches |
publishDate |
2023 |
url |
https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867_1/component/file_5022935/5022867.pdf |
genre |
Greenland Ice Sheet |
genre_facet |
Greenland Ice Sheet |
op_source |
FEMS Microbiology Ecology |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1093/femsec/fiad119 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5022867_1/component/file_5022935/5022867.pdf |
op_rights |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ |
op_doi |
https://doi.org/10.1093/femsec/fiad119 |
container_title |
FEMS Microbiology Ecology |
container_volume |
99 |
container_issue |
11 |
_version_ |
1782334606747369472 |