Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica)
The James Ross archipelago houses numerous lakes and ponds. In this region, a vast diatom and cyanobacterial variety has been reported; however, the prokaryotic diversity in microbial mats from these lakes remains poorly explored. Here, a high- throughput sequencing of 16S rRNA gene in microbial mat...
Published in: | Polar Biology |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | unknown |
Published: |
2022
|
Subjects: | |
Online Access: | https://gfzpublic.gfz-potsdam.de/pubman/item/item_5009970 |
id |
ftgfzpotsdam:oai:gfzpublic.gfz-potsdam.de:item_5009970 |
---|---|
record_format |
openpolar |
spelling |
ftgfzpotsdam:oai:gfzpublic.gfz-potsdam.de:item_5009970 2023-05-15T14:02:24+02:00 Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica) Fernández, G. Lecomte, K. Vignoni, P. Rueda, E. Coria, S. Lirio, J. Mlewski, E. 2022 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5009970 unknown info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-021-02997-z https://gfzpublic.gfz-potsdam.de/pubman/item/item_5009970 Polar Biology info:eu-repo/semantics/article 2022 ftgfzpotsdam https://doi.org/10.1007/s00300-021-02997-z 2022-09-14T05:58:06Z The James Ross archipelago houses numerous lakes and ponds. In this region, a vast diatom and cyanobacterial variety has been reported; however, the prokaryotic diversity in microbial mats from these lakes remains poorly explored. Here, a high- throughput sequencing of 16S rRNA gene in microbial mats from Lake Bart-Roja in James Ross Island and lakes Pan Negro and North Pan Negro located in Vega Island was performed. Combined with mineralogical and environmental characteristics, we analyzed the diversity and structure of the microbial communities. Sequences assigned to Archaea were extremely low, while Bacteria domain prevailed with the abundance of Proteobacteria (mostly Betaproteobacteriales) followed by Bacte- roidetes, Verrucomicrobia, Firmicutes, and Cyanobacteria. Local environmental conditions, such as conductivity and Eh, provided differential microbial assemblages that might have implications in the oligotrophic status of the lakes. Consequently, a clear segregation at the family level was observed. In this sense, the assigned diversity was related to taxa recognized as denitrifiers and sulfur oxidizers. Particularly, in Lake Pan Negro sulfur-reducing and methanogenic representatives were also found and positively correlate with alkalinity and water depth. Moreover, Deinococcus-Thermus was observed in Lake Bart-Roja, while Melainabacteria (Cyanobacteria)—poorly reported in Antarctic mats—was detected in Lake Pan Negro. Epsilonbacteraeota was exclusively found in this lake, suggesting new potential phylotypes. This study contributes to the understanding of the diversity, composition, and structure of Antarctic benthic microbial ecosystems and provides highly valuable information, which can be used as a proxy to evaluate environmental changes affecting Antarctic microbiota. Article in Journal/Newspaper Antarc* Antarctic Antarctica James Ross Island Polar Biology Ross Archipelago Ross Island Vega Island West Antarctica GFZpublic (German Research Centre for Geosciences, Helmholtz-Zentrum Potsdam) Antarctic West Antarctica Ross Island Roja ENVELOPE(-67.150,-67.150,-68.317,-68.317) Vega Island ENVELOPE(-57.500,-57.500,-63.833,-63.833) Ross Archipelago ENVELOPE(167.000,167.000,-77.500,-77.500) Polar Biology |
institution |
Open Polar |
collection |
GFZpublic (German Research Centre for Geosciences, Helmholtz-Zentrum Potsdam) |
op_collection_id |
ftgfzpotsdam |
language |
unknown |
description |
The James Ross archipelago houses numerous lakes and ponds. In this region, a vast diatom and cyanobacterial variety has been reported; however, the prokaryotic diversity in microbial mats from these lakes remains poorly explored. Here, a high- throughput sequencing of 16S rRNA gene in microbial mats from Lake Bart-Roja in James Ross Island and lakes Pan Negro and North Pan Negro located in Vega Island was performed. Combined with mineralogical and environmental characteristics, we analyzed the diversity and structure of the microbial communities. Sequences assigned to Archaea were extremely low, while Bacteria domain prevailed with the abundance of Proteobacteria (mostly Betaproteobacteriales) followed by Bacte- roidetes, Verrucomicrobia, Firmicutes, and Cyanobacteria. Local environmental conditions, such as conductivity and Eh, provided differential microbial assemblages that might have implications in the oligotrophic status of the lakes. Consequently, a clear segregation at the family level was observed. In this sense, the assigned diversity was related to taxa recognized as denitrifiers and sulfur oxidizers. Particularly, in Lake Pan Negro sulfur-reducing and methanogenic representatives were also found and positively correlate with alkalinity and water depth. Moreover, Deinococcus-Thermus was observed in Lake Bart-Roja, while Melainabacteria (Cyanobacteria)—poorly reported in Antarctic mats—was detected in Lake Pan Negro. Epsilonbacteraeota was exclusively found in this lake, suggesting new potential phylotypes. This study contributes to the understanding of the diversity, composition, and structure of Antarctic benthic microbial ecosystems and provides highly valuable information, which can be used as a proxy to evaluate environmental changes affecting Antarctic microbiota. |
format |
Article in Journal/Newspaper |
author |
Fernández, G. Lecomte, K. Vignoni, P. Rueda, E. Coria, S. Lirio, J. Mlewski, E. |
spellingShingle |
Fernández, G. Lecomte, K. Vignoni, P. Rueda, E. Coria, S. Lirio, J. Mlewski, E. Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica) |
author_facet |
Fernández, G. Lecomte, K. Vignoni, P. Rueda, E. Coria, S. Lirio, J. Mlewski, E. |
author_sort |
Fernández, G. |
title |
Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica) |
title_short |
Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica) |
title_full |
Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica) |
title_fullStr |
Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica) |
title_full_unstemmed |
Prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from James Ross Archipelago (West Antarctica) |
title_sort |
prokaryotic diversity and biogeochemical characteristics of benthic microbial ecosystems from james ross archipelago (west antarctica) |
publishDate |
2022 |
url |
https://gfzpublic.gfz-potsdam.de/pubman/item/item_5009970 |
long_lat |
ENVELOPE(-67.150,-67.150,-68.317,-68.317) ENVELOPE(-57.500,-57.500,-63.833,-63.833) ENVELOPE(167.000,167.000,-77.500,-77.500) |
geographic |
Antarctic West Antarctica Ross Island Roja Vega Island Ross Archipelago |
geographic_facet |
Antarctic West Antarctica Ross Island Roja Vega Island Ross Archipelago |
genre |
Antarc* Antarctic Antarctica James Ross Island Polar Biology Ross Archipelago Ross Island Vega Island West Antarctica |
genre_facet |
Antarc* Antarctic Antarctica James Ross Island Polar Biology Ross Archipelago Ross Island Vega Island West Antarctica |
op_source |
Polar Biology |
op_relation |
info:eu-repo/semantics/altIdentifier/doi/10.1007/s00300-021-02997-z https://gfzpublic.gfz-potsdam.de/pubman/item/item_5009970 |
op_doi |
https://doi.org/10.1007/s00300-021-02997-z |
container_title |
Polar Biology |
_version_ |
1766272663300341760 |