Methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw
This thesis investigates how the permafrost microbiota responds to global warming. In detail, the constraints behind methane production in thawing permafrost were linked to methanogenic activity, abundance and composition. Furthermore, this thesis offers new insights into microbial adaptions to the...
Main Author: | |
---|---|
Format: | Doctoral or Postdoctoral Thesis |
Language: | English |
Published: |
Universität Potsdam
2020
|
Subjects: | |
Online Access: | https://gfzpublic.gfz-potsdam.de/pubman/item/item_5005971 |
id |
ftgfzpotsdam:oai:gfzpublic.gfz-potsdam.de:item_5005971 |
---|---|
record_format |
openpolar |
spelling |
ftgfzpotsdam:oai:gfzpublic.gfz-potsdam.de:item_5005971 2023-05-15T17:55:41+02:00 Methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw Holm, S. 2020 https://gfzpublic.gfz-potsdam.de/pubman/item/item_5005971 eng eng Universität Potsdam https://gfzpublic.gfz-potsdam.de/pubman/item/item_5005971 info:eu-repo/semantics/doctoralThesis 2020 ftgfzpotsdam 2022-09-14T05:57:43Z This thesis investigates how the permafrost microbiota responds to global warming. In detail, the constraints behind methane production in thawing permafrost were linked to methanogenic activity, abundance and composition. Furthermore, this thesis offers new insights into microbial adaptions to the changing environmental conditions during global warming. This was assesed by investigating the potential ecological relevant functions encoded by plasmid DNA within the permafrost microbiota. Permafrost of both interglacial and glacial origin spanning the Holocene to the late Pleistocene, including Eemian, were studied during long-term thaw incubations. Furthermore, several permafrost cores of different stratigraphy, soil type and vegetation cover were used to target the main constraints behind methane production during short-term thaw simulations. Short- and long-term incubations simulating thaw with and without the addition of substrate were combined with activity measurements, amplicon and metagenomic sequencing of permanently frozen and seasonally thawed active layer. Combined, it allowed to address the following questions. i) What constraints methane production when permafrost thaws and how is this linked to methanogenic activity, abundance and composition? ii) How does the methanogenic community composition change during long-term thawing conditions? iii) Which potential ecological relevant functions are encoded by plasmid DNA in active layer soils? The major outcomes of this thesis are as follows. i) Methane production from permafrost after long-term thaw simulation was found to be constrained mainly by the abundance of methanogens and the archaeal community composition. Deposits formed during periods of warmer temperatures and increased precipitation, (here represented by deposits from the Late Pleistocene of both interstadial and interglacial periods) were found to respond strongest to thawing conditions and to contain an archaeal community dominated by methanogenic archaea (40% and 100% of all detected ... Doctoral or Postdoctoral Thesis permafrost GFZpublic (German Research Centre for Geosciences, Helmholtz-Zentrum Potsdam) |
institution |
Open Polar |
collection |
GFZpublic (German Research Centre for Geosciences, Helmholtz-Zentrum Potsdam) |
op_collection_id |
ftgfzpotsdam |
language |
English |
description |
This thesis investigates how the permafrost microbiota responds to global warming. In detail, the constraints behind methane production in thawing permafrost were linked to methanogenic activity, abundance and composition. Furthermore, this thesis offers new insights into microbial adaptions to the changing environmental conditions during global warming. This was assesed by investigating the potential ecological relevant functions encoded by plasmid DNA within the permafrost microbiota. Permafrost of both interglacial and glacial origin spanning the Holocene to the late Pleistocene, including Eemian, were studied during long-term thaw incubations. Furthermore, several permafrost cores of different stratigraphy, soil type and vegetation cover were used to target the main constraints behind methane production during short-term thaw simulations. Short- and long-term incubations simulating thaw with and without the addition of substrate were combined with activity measurements, amplicon and metagenomic sequencing of permanently frozen and seasonally thawed active layer. Combined, it allowed to address the following questions. i) What constraints methane production when permafrost thaws and how is this linked to methanogenic activity, abundance and composition? ii) How does the methanogenic community composition change during long-term thawing conditions? iii) Which potential ecological relevant functions are encoded by plasmid DNA in active layer soils? The major outcomes of this thesis are as follows. i) Methane production from permafrost after long-term thaw simulation was found to be constrained mainly by the abundance of methanogens and the archaeal community composition. Deposits formed during periods of warmer temperatures and increased precipitation, (here represented by deposits from the Late Pleistocene of both interstadial and interglacial periods) were found to respond strongest to thawing conditions and to contain an archaeal community dominated by methanogenic archaea (40% and 100% of all detected ... |
format |
Doctoral or Postdoctoral Thesis |
author |
Holm, S. |
spellingShingle |
Holm, S. Methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw |
author_facet |
Holm, S. |
author_sort |
Holm, S. |
title |
Methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw |
title_short |
Methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw |
title_full |
Methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw |
title_fullStr |
Methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw |
title_full_unstemmed |
Methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw |
title_sort |
methanogenic communities and metaplasmidome-encoded functions in permafrost environments exposed to thaw |
publisher |
Universität Potsdam |
publishDate |
2020 |
url |
https://gfzpublic.gfz-potsdam.de/pubman/item/item_5005971 |
genre |
permafrost |
genre_facet |
permafrost |
op_relation |
https://gfzpublic.gfz-potsdam.de/pubman/item/item_5005971 |
_version_ |
1766163664028565504 |