Discovery and Characterization of a New Cold-Active Protease From an Extremophilic Bacterium via Comparative Genome Analysis and in vitro Expression

• Published in: Frontiers in Microbiology
• Main Authors: Perfumo, A., Freiherr von Sass, G., Nordmann, E., Budisa, N., Wagner, D.
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: 2020
• Subjects: Antarctic; Triton; Antarc*
• Online Access: https://gfzpublic.gfz-potsdam.de/pubman/item/item_5001917; https://gfzpublic.gfz-potsdam.de/pubman/item/item_5001917_2/component/file_5001926/5001917.pdf

Following a screening of Antarctic glacier forefield-bacteria for novel cold-active enzymes, a psychrophilic strain Psychrobacter sp. 94-6PB was selected for further characterization of enzymatic activities. The strain produced lipases and proteases in the temperature range of 4–18°C. The coding seq...