Salinity Adaptation and Gene Profiling Analysis in the European Eel (Anguilla anguilla) Using Microarray Technology
The life cycle of the European eel (Anguilla anguilla) includes two long migratory periods, when the newly hatched leptocephali larvae drift on ocean currents from the Sargasso Sea to the shores of Western Europe and then again up to 30 years later when adult eels swim back to their place of birth f...
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ftgeorgiasouth:oai:digitalcommons.georgiasouthern.edu:biology-facpubs-1053 2023-09-26T15:09:23+02:00 Salinity Adaptation and Gene Profiling Analysis in the European Eel (Anguilla anguilla) Using Microarray Technology Kalujnaia, Svetlana McWilliam, Ian S. Zaguinaiko, Vitalii A. Feilen, Anya L. Nicholson, John Hazon, Neil Cutler, Christopher P. Balment, Richard J. Cossins, Andrew R. Hughes, Margaret Cramb, Gordon 2007-06-01T07:00:00Z https://digitalcommons.georgiasouthern.edu/biology-facpubs/45 https://doi.org/10.1016/j.ygcen.2006.12.025 unknown Digital Commons@Georgia Southern https://digitalcommons.georgiasouthern.edu/biology-facpubs/45 doi:10.1016/j.ygcen.2006.12.025 https://doi.org/10.1016/j.ygcen.2006.12.025 Copyright belongs to Elsevier. Information regarding the dissemination and usage of journal articles can be accessed through the following links. Open access licenses Article Sharing Journal Embargo Period List Department of Biology Faculty Publications Salinity adaptation Gene profiling analysis European eel Anguilla anguilla Microarray technology Biochemistry Biophysics and Structural Biology Biology text 2007 ftgeorgiasouth https://doi.org/10.1016/j.ygcen.2006.12.025 2023-08-27T22:39:53Z The life cycle of the European eel (Anguilla anguilla) includes two long migratory periods, when the newly hatched leptocephali larvae drift on ocean currents from the Sargasso Sea to the shores of Western Europe and then again up to 30 years later when adult eels swim back to their place of birth for reproductive purposes. Prior to the migration from fresh water (FW) to sea water (SW) adult yellow eels undergo various anatomical and physiological adaptations (silvering) which promote sexual development and aid the transition to increased environmental salinities. The aim of this study was to identify and characterise changes in gene expression within the major osmoregulatory tissues of the eel which enable these fish to make the physiological adaptations required for transfer to SW environments. In particular, changes in the expression of the FW-adapting hormone prolactin were correlated with differential expression of known osmoregulatory important genes within the gill, intestine and kidney following the acclimation of eels to SW. Various tissues were sampled from individual fish at selected intervals over a 5-month period following FW/SW transfer and RNA was isolated. Suppressive subtractive hybridization (SSH) was used for enrichment of differentially expressed genes. Microarrays comprising 6144 cDNAs spotted in triplicate, from brain, gill, intestine and kidney libraries (1536 randomly selected clones per tissue library), were hybridized with appropriate targets and analysed. Microarray results were validated using known genes implicated in osmoregulation, such as prolactin, growth hormone, Na, K-ATPase and some unknown genes, the role of which in osmoregulation needs to be elucidated. Text Anguilla anguilla Georgia Southern University: Digital Commons@Georgia Southern General and Comparative Endocrinology 152 2-3 274 280 |
institution |
Open Polar |
collection |
Georgia Southern University: Digital Commons@Georgia Southern |
op_collection_id |
ftgeorgiasouth |
language |
unknown |
topic |
Salinity adaptation Gene profiling analysis European eel Anguilla anguilla Microarray technology Biochemistry Biophysics and Structural Biology Biology |
spellingShingle |
Salinity adaptation Gene profiling analysis European eel Anguilla anguilla Microarray technology Biochemistry Biophysics and Structural Biology Biology Kalujnaia, Svetlana McWilliam, Ian S. Zaguinaiko, Vitalii A. Feilen, Anya L. Nicholson, John Hazon, Neil Cutler, Christopher P. Balment, Richard J. Cossins, Andrew R. Hughes, Margaret Cramb, Gordon Salinity Adaptation and Gene Profiling Analysis in the European Eel (Anguilla anguilla) Using Microarray Technology |
topic_facet |
Salinity adaptation Gene profiling analysis European eel Anguilla anguilla Microarray technology Biochemistry Biophysics and Structural Biology Biology |
description |
The life cycle of the European eel (Anguilla anguilla) includes two long migratory periods, when the newly hatched leptocephali larvae drift on ocean currents from the Sargasso Sea to the shores of Western Europe and then again up to 30 years later when adult eels swim back to their place of birth for reproductive purposes. Prior to the migration from fresh water (FW) to sea water (SW) adult yellow eels undergo various anatomical and physiological adaptations (silvering) which promote sexual development and aid the transition to increased environmental salinities. The aim of this study was to identify and characterise changes in gene expression within the major osmoregulatory tissues of the eel which enable these fish to make the physiological adaptations required for transfer to SW environments. In particular, changes in the expression of the FW-adapting hormone prolactin were correlated with differential expression of known osmoregulatory important genes within the gill, intestine and kidney following the acclimation of eels to SW. Various tissues were sampled from individual fish at selected intervals over a 5-month period following FW/SW transfer and RNA was isolated. Suppressive subtractive hybridization (SSH) was used for enrichment of differentially expressed genes. Microarrays comprising 6144 cDNAs spotted in triplicate, from brain, gill, intestine and kidney libraries (1536 randomly selected clones per tissue library), were hybridized with appropriate targets and analysed. Microarray results were validated using known genes implicated in osmoregulation, such as prolactin, growth hormone, Na, K-ATPase and some unknown genes, the role of which in osmoregulation needs to be elucidated. |
format |
Text |
author |
Kalujnaia, Svetlana McWilliam, Ian S. Zaguinaiko, Vitalii A. Feilen, Anya L. Nicholson, John Hazon, Neil Cutler, Christopher P. Balment, Richard J. Cossins, Andrew R. Hughes, Margaret Cramb, Gordon |
author_facet |
Kalujnaia, Svetlana McWilliam, Ian S. Zaguinaiko, Vitalii A. Feilen, Anya L. Nicholson, John Hazon, Neil Cutler, Christopher P. Balment, Richard J. Cossins, Andrew R. Hughes, Margaret Cramb, Gordon |
author_sort |
Kalujnaia, Svetlana |
title |
Salinity Adaptation and Gene Profiling Analysis in the European Eel (Anguilla anguilla) Using Microarray Technology |
title_short |
Salinity Adaptation and Gene Profiling Analysis in the European Eel (Anguilla anguilla) Using Microarray Technology |
title_full |
Salinity Adaptation and Gene Profiling Analysis in the European Eel (Anguilla anguilla) Using Microarray Technology |
title_fullStr |
Salinity Adaptation and Gene Profiling Analysis in the European Eel (Anguilla anguilla) Using Microarray Technology |
title_full_unstemmed |
Salinity Adaptation and Gene Profiling Analysis in the European Eel (Anguilla anguilla) Using Microarray Technology |
title_sort |
salinity adaptation and gene profiling analysis in the european eel (anguilla anguilla) using microarray technology |
publisher |
Digital Commons@Georgia Southern |
publishDate |
2007 |
url |
https://digitalcommons.georgiasouthern.edu/biology-facpubs/45 https://doi.org/10.1016/j.ygcen.2006.12.025 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
Department of Biology Faculty Publications |
op_relation |
https://digitalcommons.georgiasouthern.edu/biology-facpubs/45 doi:10.1016/j.ygcen.2006.12.025 https://doi.org/10.1016/j.ygcen.2006.12.025 |
op_rights |
Copyright belongs to Elsevier. Information regarding the dissemination and usage of journal articles can be accessed through the following links. Open access licenses Article Sharing Journal Embargo Period List |
op_doi |
https://doi.org/10.1016/j.ygcen.2006.12.025 |
container_title |
General and Comparative Endocrinology |
container_volume |
152 |
container_issue |
2-3 |
container_start_page |
274 |
op_container_end_page |
280 |
_version_ |
1778135215023063040 |