A Transcriptomic Approach to the Study of Osmoregulation in European Eel Anguilla anguilla
In euryhaline teleosts, osmoregulation is a fundamental and dynamic process that is essential for the maintenance of ion and water balance, especially when fish migrate between fresh water (FW) and sea water (SW) environments. The European eel has proved to be an excellent model species to study the...
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ftgeorgiasouth:oai:digitalcommons.georgiasouthern.edu:biology-facpubs-1052 2023-09-26T15:09:25+02:00 A Transcriptomic Approach to the Study of Osmoregulation in European Eel Anguilla anguilla Kalujnaia, Svetlana McWilliam, Ian S. Zaguinaiko, Vitalii A. Feilen, Anja L. Nicholson, John Hazon, Neil Cutler, Christopher P. Cramb, Gordon 2007-11-01T07:00:00Z https://digitalcommons.georgiasouthern.edu/biology-facpubs/46 https://doi.org/10.1152/physiolgenomics.00059.2007 unknown Digital Commons@Georgia Southern https://digitalcommons.georgiasouthern.edu/biology-facpubs/46 doi:10.1152/physiolgenomics.00059.2007 https://doi.org/10.1152/physiolgenomics.00059.2007 Department of Biology Faculty Publications Transcriptomic approach Study Osmoregulation European ell Anguilla anguilla Biochemistry Biophysics and Structural Biology Biology text 2007 ftgeorgiasouth https://doi.org/10.1152/physiolgenomics.00059.2007 2023-08-27T21:40:49Z In euryhaline teleosts, osmoregulation is a fundamental and dynamic process that is essential for the maintenance of ion and water balance, especially when fish migrate between fresh water (FW) and sea water (SW) environments. The European eel has proved to be an excellent model species to study the molecular and physiological adaptations associated with this osmoregulatory plasticity. The life cycle of the European eel includes two migratory periods, the second being the migration of FW eels back to the Sargasso Sea for reproduction. Various anatomical and physiological changes allow the successful transition to SW. The aim of this study was to use a microarray approach to screen the osmoregulatory tissues of the eel for changes in gene expression following acclimation to SW. Tissues were sampled from fish at selected intervals over a 5-mo period following FW/SW transfer, and RNA was isolated. Suppressive subtractive hybridization was used for enrichment of differentially expressed genes. Microarrays comprising 6,144 cDNAs from brain, gill, intestine, and kidney libraries were hybridized with appropriate targets and analyzed; 229 differentially expressed clones with unique sequences were identified. These clones represented the sequences for 95 known genes, with the remaining sequences (59%) being unknown. The results of the microarray analysis were validated by quantification of 28 differentially expressed genes by Northern blotting. A number of the differentially expressed genes were already known to be involved in osmoregulation, but the functional roles of many others, not normally associated with ion or water transport, remain to be characterized. Text Anguilla anguilla European eel Georgia Southern University: Digital Commons@Georgia Southern Physiological Genomics 31 3 385 401 |
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Georgia Southern University: Digital Commons@Georgia Southern |
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ftgeorgiasouth |
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unknown |
topic |
Transcriptomic approach Study Osmoregulation European ell Anguilla anguilla Biochemistry Biophysics and Structural Biology Biology |
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Transcriptomic approach Study Osmoregulation European ell Anguilla anguilla Biochemistry Biophysics and Structural Biology Biology Kalujnaia, Svetlana McWilliam, Ian S. Zaguinaiko, Vitalii A. Feilen, Anja L. Nicholson, John Hazon, Neil Cutler, Christopher P. Cramb, Gordon A Transcriptomic Approach to the Study of Osmoregulation in European Eel Anguilla anguilla |
topic_facet |
Transcriptomic approach Study Osmoregulation European ell Anguilla anguilla Biochemistry Biophysics and Structural Biology Biology |
description |
In euryhaline teleosts, osmoregulation is a fundamental and dynamic process that is essential for the maintenance of ion and water balance, especially when fish migrate between fresh water (FW) and sea water (SW) environments. The European eel has proved to be an excellent model species to study the molecular and physiological adaptations associated with this osmoregulatory plasticity. The life cycle of the European eel includes two migratory periods, the second being the migration of FW eels back to the Sargasso Sea for reproduction. Various anatomical and physiological changes allow the successful transition to SW. The aim of this study was to use a microarray approach to screen the osmoregulatory tissues of the eel for changes in gene expression following acclimation to SW. Tissues were sampled from fish at selected intervals over a 5-mo period following FW/SW transfer, and RNA was isolated. Suppressive subtractive hybridization was used for enrichment of differentially expressed genes. Microarrays comprising 6,144 cDNAs from brain, gill, intestine, and kidney libraries were hybridized with appropriate targets and analyzed; 229 differentially expressed clones with unique sequences were identified. These clones represented the sequences for 95 known genes, with the remaining sequences (59%) being unknown. The results of the microarray analysis were validated by quantification of 28 differentially expressed genes by Northern blotting. A number of the differentially expressed genes were already known to be involved in osmoregulation, but the functional roles of many others, not normally associated with ion or water transport, remain to be characterized. |
format |
Text |
author |
Kalujnaia, Svetlana McWilliam, Ian S. Zaguinaiko, Vitalii A. Feilen, Anja L. Nicholson, John Hazon, Neil Cutler, Christopher P. Cramb, Gordon |
author_facet |
Kalujnaia, Svetlana McWilliam, Ian S. Zaguinaiko, Vitalii A. Feilen, Anja L. Nicholson, John Hazon, Neil Cutler, Christopher P. Cramb, Gordon |
author_sort |
Kalujnaia, Svetlana |
title |
A Transcriptomic Approach to the Study of Osmoregulation in European Eel Anguilla anguilla |
title_short |
A Transcriptomic Approach to the Study of Osmoregulation in European Eel Anguilla anguilla |
title_full |
A Transcriptomic Approach to the Study of Osmoregulation in European Eel Anguilla anguilla |
title_fullStr |
A Transcriptomic Approach to the Study of Osmoregulation in European Eel Anguilla anguilla |
title_full_unstemmed |
A Transcriptomic Approach to the Study of Osmoregulation in European Eel Anguilla anguilla |
title_sort |
transcriptomic approach to the study of osmoregulation in european eel anguilla anguilla |
publisher |
Digital Commons@Georgia Southern |
publishDate |
2007 |
url |
https://digitalcommons.georgiasouthern.edu/biology-facpubs/46 https://doi.org/10.1152/physiolgenomics.00059.2007 |
genre |
Anguilla anguilla European eel |
genre_facet |
Anguilla anguilla European eel |
op_source |
Department of Biology Faculty Publications |
op_relation |
https://digitalcommons.georgiasouthern.edu/biology-facpubs/46 doi:10.1152/physiolgenomics.00059.2007 https://doi.org/10.1152/physiolgenomics.00059.2007 |
op_doi |
https://doi.org/10.1152/physiolgenomics.00059.2007 |
container_title |
Physiological Genomics |
container_volume |
31 |
container_issue |
3 |
container_start_page |
385 |
op_container_end_page |
401 |
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1778135590626131968 |