Wechselwirkungen zwischen der antarktischen Eisdiatomee Amphiprora kufferathii Manguin und epiphytischen Bakterien unter besonderer Berücksichtigung des Oxidatinsschutzes

• Other Authors: Hünken, Mark
• Language: German
• Published: Staats- und Universitätsbibliothek [Host] 2004
• Subjects: Antarctic; The Antarctic; Antarc*; Antarktis*; Sea ice
• Online Access: http://nbn-resolving.de/urn:nbn:de:gbv:46-diss000010534; http://webdoc.sub.gwdg.de/ebook/diss/Bremen/2004/E-Diss1053_Hünken.pdf

Hydrogen peroxide, symbiosis, species-spanned antioxidative defence system, catalase, superoxide dismutase, glutathione reductase, sea ice. - The Antarctic ice diatom Amphiprora kufferathii is covered by epibacteria in its natural environment. Axenic cultures of the diatom were established with the antibiotic ampicilline. The experiments were carried out with bacterized and axenic cultures of the diatom.The bacteria were identified by isolation and cloning of total 16S rRNS of the bacterized diatom culture. Phylogenetic analysis showed that A. kufferathii is covered by alphaproteobacteria of the genera Octadecabacter and Sulfitobacter as well as by members of the bacteriodetes phylum of the genera Cellulophaga and Psychroflexus. Most of the free living bacteria were classified as gammaproteobacteria of the genera Colwellia. Cellulophaga, Sulfitobacter, and Colwellia were isolated from the diatom culture and used for further studies.Quantitative fluorescence in situ hybridization showed a 70% coverage of the diatom by Octadecabacter. Bacteriodetes and gammaproteobacteria showed a maximal abundance of 10% to 13%.The epibacteria stimulate the growth of A. kufferathii. Experiments under nutrient limitation . @Bremen, Univ., Diss., 2004