Global distribution of a chlorophyll f cyanobacterial marker
Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging fr...
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ftfuberlin:oai:refubium.fu-berlin.de:fub188/30006 2023-05-15T14:00:30+02:00 Global distribution of a chlorophyll f cyanobacterial marker Antonaru, Laura A. Cardona, Tanai Larkum, Anthony W. D. Nürnberg, Dennis J. 2020 13 Seiten application/pdf application/vnd.openxmlformats-officedocument.wordprocessingml.document https://refubium.fu-berlin.de/handle/fub188/30006 https://doi.org/10.17169/refubium-29748 https://doi.org/10.1038/s41396-020-0670-y eng eng https://refubium.fu-berlin.de/handle/fub188/30006 http://dx.doi.org/10.17169/refubium-29748 80078 doi:10.1038/s41396-020-0670-y https://creativecommons.org/licenses/by/4.0/ CC-BY Environmental microbiology Microbial ecology ddc:530 ddc:577 doc-type:article 2020 ftfuberlin https://doi.org/10.17169/refubium-29748 https://doi.org/10.1038/s41396-020-0670-y 2022-05-15T20:50:28Z Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype’s distribution. Article in Journal/Newspaper Antarc* Antarctic Arctic Freie Universität Berlin: Refubium (FU Berlin) Antarctic Arctic |
institution |
Open Polar |
collection |
Freie Universität Berlin: Refubium (FU Berlin) |
op_collection_id |
ftfuberlin |
language |
English |
topic |
Environmental microbiology Microbial ecology ddc:530 ddc:577 |
spellingShingle |
Environmental microbiology Microbial ecology ddc:530 ddc:577 Antonaru, Laura A. Cardona, Tanai Larkum, Anthony W. D. Nürnberg, Dennis J. Global distribution of a chlorophyll f cyanobacterial marker |
topic_facet |
Environmental microbiology Microbial ecology ddc:530 ddc:577 |
description |
Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype’s distribution. |
format |
Article in Journal/Newspaper |
author |
Antonaru, Laura A. Cardona, Tanai Larkum, Anthony W. D. Nürnberg, Dennis J. |
author_facet |
Antonaru, Laura A. Cardona, Tanai Larkum, Anthony W. D. Nürnberg, Dennis J. |
author_sort |
Antonaru, Laura A. |
title |
Global distribution of a chlorophyll f cyanobacterial marker |
title_short |
Global distribution of a chlorophyll f cyanobacterial marker |
title_full |
Global distribution of a chlorophyll f cyanobacterial marker |
title_fullStr |
Global distribution of a chlorophyll f cyanobacterial marker |
title_full_unstemmed |
Global distribution of a chlorophyll f cyanobacterial marker |
title_sort |
global distribution of a chlorophyll f cyanobacterial marker |
publishDate |
2020 |
url |
https://refubium.fu-berlin.de/handle/fub188/30006 https://doi.org/10.17169/refubium-29748 https://doi.org/10.1038/s41396-020-0670-y |
geographic |
Antarctic Arctic |
geographic_facet |
Antarctic Arctic |
genre |
Antarc* Antarctic Arctic |
genre_facet |
Antarc* Antarctic Arctic |
op_relation |
https://refubium.fu-berlin.de/handle/fub188/30006 http://dx.doi.org/10.17169/refubium-29748 80078 doi:10.1038/s41396-020-0670-y |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.17169/refubium-29748 https://doi.org/10.1038/s41396-020-0670-y |
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1766269673684336640 |