Metagenomic insights into diazotrophic communities across Arctic glacier forefields
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In to...
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ftfuberlin:oai:refubium.fu-berlin.de:fub188/22837 2023-05-15T14:51:42+02:00 Metagenomic insights into diazotrophic communities across Arctic glacier forefields Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. S´anchez-Baracaldo, Patricia 2018 12 S. application/pdf https://refubium.fu-berlin.de/handle/fub188/22837 https://doi.org/10.17169/refubium-636 https://doi.org/10.1093/femsec/fiy114 eng eng https://refubium.fu-berlin.de/handle/fub188/22837 http://dx.doi.org/10.17169/refubium-636 doi:10.1093/femsec/fiy114 https://creativecommons.org/licenses/by/4.0/ CC-BY nitrogen fixation metagenomics forefield Arctic diversity diazotrophs ddc:579 doc-type:article 2018 ftfuberlin https://doi.org/10.17169/refubium-636 https://doi.org/10.1093/femsec/fiy114 2022-05-15T20:50:13Z Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. Article in Journal/Newspaper Arctic Freie Universität Berlin: Refubium (FU Berlin) Arctic |
institution |
Open Polar |
collection |
Freie Universität Berlin: Refubium (FU Berlin) |
op_collection_id |
ftfuberlin |
language |
English |
topic |
nitrogen fixation metagenomics forefield Arctic diversity diazotrophs ddc:579 |
spellingShingle |
nitrogen fixation metagenomics forefield Arctic diversity diazotrophs ddc:579 Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. S´anchez-Baracaldo, Patricia Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
topic_facet |
nitrogen fixation metagenomics forefield Arctic diversity diazotrophs ddc:579 |
description |
Microbial nitrogen fixation is crucial for building labile nitrogen stocks and facilitating higher plant colonisation in oligotrophic glacier forefield soils. Here, the diazotrophic bacterial community structure across four Arctic glacier forefields was investigated using metagenomic analysis. In total, 70 soil metagenomes were used for taxonomic interpretation based on 185 nitrogenase (nif) sequences, extracted from assembled contigs. The low number of recovered genes highlights the need for deeper sequencing in some diverse samples, to uncover the complete microbial populations. A key group of forefield diazotrophs, found throughout the forefields, was identified using a nifH phylogeny, associated with nifH Cluster I and III. Sequences related most closely to groups including Alphaproteobacteria, Betaproteobacteria, Cyanobacteria and Firmicutes. Using multiple nif genes in a Last Common Ancestor analysis revealed a diverse range of diazotrophs across the forefields. Key organisms identified across the forefields included Nostoc, Geobacter, Polaromonas and Frankia. Nitrogen fixers that are symbiotic with plants were also identified, through the presence of root associated diazotrophs, which fix nitrogen in return for reduced carbon. Additional nitrogen fixers identified in forefield soils were metabolically diverse, including fermentative and sulphur cycling bacteria, halophiles and anaerobes. |
format |
Article in Journal/Newspaper |
author |
Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. S´anchez-Baracaldo, Patricia |
author_facet |
Nash, Maisie V. Anesio, Alexandre M. Barker, Gary Tranter, Martyn Varliero, Gilda Eloe-Fadrosh, Emiley A. Nielsen, Torben Turpin-Jelfs, Thomas Benning, Liane G. S´anchez-Baracaldo, Patricia |
author_sort |
Nash, Maisie V. |
title |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_short |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_fullStr |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_full_unstemmed |
Metagenomic insights into diazotrophic communities across Arctic glacier forefields |
title_sort |
metagenomic insights into diazotrophic communities across arctic glacier forefields |
publishDate |
2018 |
url |
https://refubium.fu-berlin.de/handle/fub188/22837 https://doi.org/10.17169/refubium-636 https://doi.org/10.1093/femsec/fiy114 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
https://refubium.fu-berlin.de/handle/fub188/22837 http://dx.doi.org/10.17169/refubium-636 doi:10.1093/femsec/fiy114 |
op_rights |
https://creativecommons.org/licenses/by/4.0/ |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.17169/refubium-636 https://doi.org/10.1093/femsec/fiy114 |
_version_ |
1766322821402722304 |