Image_9_From Africa to Antarctica: Exploring the Metabolism of Fish Heart Mitochondria Across a Wide Thermal Range.pdf
The thermal sensitivity of ectotherms is largely dictated by the impact of temperature on cellular bioenergetics, particularly on mitochondrial functions. As the thermal sensitivity of bioenergetic pathways depends on the structural and kinetic properties of its component enzymes, optimization of th...
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ftfrontimediafig:oai:figshare.com:article/9938468 2023-05-15T14:02:26+02:00 Image_9_From Africa to Antarctica: Exploring the Metabolism of Fish Heart Mitochondria Across a Wide Thermal Range.pdf Florence Hunter-Manseau Véronique Desrosiers Nathalie R. Le François France Dufresne H. William Detrich Christian Nozais Pierre U. Blier 2019-10-04T11:56:49Z https://doi.org/10.3389/fphys.2019.01220.s010 https://figshare.com/articles/Image_9_From_Africa_to_Antarctica_Exploring_the_Metabolism_of_Fish_Heart_Mitochondria_Across_a_Wide_Thermal_Range_pdf/9938468 unknown doi:10.3389/fphys.2019.01220.s010 https://figshare.com/articles/Image_9_From_Africa_to_Antarctica_Exploring_the_Metabolism_of_Fish_Heart_Mitochondria_Across_a_Wide_Thermal_Range_pdf/9938468 CC BY 4.0 CC-BY Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified temperature adaptation pyruvate dehydrogenase complex carnitine palmitoyl transferase hydroxyacyl-CoA dehydrogenase electron transport system energy metabolism fatty acid metabolism Image Figure 2019 ftfrontimediafig https://doi.org/10.3389/fphys.2019.01220.s010 2019-10-09T22:53:55Z The thermal sensitivity of ectotherms is largely dictated by the impact of temperature on cellular bioenergetics, particularly on mitochondrial functions. As the thermal sensitivity of bioenergetic pathways depends on the structural and kinetic properties of its component enzymes, optimization of their collective function to different thermal niches is expected to have occurred through selection. In the present study, we sought to characterize mitochondrial phenotypic adjustments to thermal niches in eight ray-finned fish species occupying a wide range of thermal habitats by comparing the activities of key mitochondrial enzymes in their hearts. We measured the activity of four enzymes that control substrate entrance into the tricarboxylic acid (TCA) cycle: pyruvate kinase (PK), pyruvate dehydrogenase complex (PDHc), carnitine palmitoyltransferase (CPT), and hydroxyacyl-CoA dehydrogenase (HOAD). We also assayed enzymes of the electron transport system (ETS): complexes I, II, I + III, and IV. Enzymes were assayed at five temperatures (5, 10, 15, 20, and 25°C). Our results showed that the activity of CPT, a gatekeeper of the fatty acid pathway, was higher in the cold-water fish than in the warmer-adapted fish relative to the ETS (complexes I and III) when measured close to the species optimal temperatures. The activity of HOAD showed a similar pattern relative to CI + III and thermal environment. By contrast, PDHc and PK did not show the similar patterns with respect to CI + III and temperature. Cold-adapted species had high CIV activities compared to those of upstream complexes (I, II, I + III) whereas the converse was true for warm-adapted species. Our findings reveal a significant variability of heart mitochondrial organization among species that can be linked to temperature adaptation. Cold-adapted fish do not appear to compensate for PDHc activity but likely adjust fatty acids oxidation through higher activities of CPT and HOAD relative to complexes I + III. Still Image Antarc* Antarctica Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified temperature adaptation pyruvate dehydrogenase complex carnitine palmitoyl transferase hydroxyacyl-CoA dehydrogenase electron transport system energy metabolism fatty acid metabolism |
spellingShingle |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified temperature adaptation pyruvate dehydrogenase complex carnitine palmitoyl transferase hydroxyacyl-CoA dehydrogenase electron transport system energy metabolism fatty acid metabolism Florence Hunter-Manseau Véronique Desrosiers Nathalie R. Le François France Dufresne H. William Detrich Christian Nozais Pierre U. Blier Image_9_From Africa to Antarctica: Exploring the Metabolism of Fish Heart Mitochondria Across a Wide Thermal Range.pdf |
topic_facet |
Physiology Exercise Physiology Nutritional Physiology Reproduction Cell Physiology Systems Physiology Animal Physiology - Biophysics Animal Physiology - Cell Animal Physiology - Systems Comparative Physiology Physiology not elsewhere classified temperature adaptation pyruvate dehydrogenase complex carnitine palmitoyl transferase hydroxyacyl-CoA dehydrogenase electron transport system energy metabolism fatty acid metabolism |
description |
The thermal sensitivity of ectotherms is largely dictated by the impact of temperature on cellular bioenergetics, particularly on mitochondrial functions. As the thermal sensitivity of bioenergetic pathways depends on the structural and kinetic properties of its component enzymes, optimization of their collective function to different thermal niches is expected to have occurred through selection. In the present study, we sought to characterize mitochondrial phenotypic adjustments to thermal niches in eight ray-finned fish species occupying a wide range of thermal habitats by comparing the activities of key mitochondrial enzymes in their hearts. We measured the activity of four enzymes that control substrate entrance into the tricarboxylic acid (TCA) cycle: pyruvate kinase (PK), pyruvate dehydrogenase complex (PDHc), carnitine palmitoyltransferase (CPT), and hydroxyacyl-CoA dehydrogenase (HOAD). We also assayed enzymes of the electron transport system (ETS): complexes I, II, I + III, and IV. Enzymes were assayed at five temperatures (5, 10, 15, 20, and 25°C). Our results showed that the activity of CPT, a gatekeeper of the fatty acid pathway, was higher in the cold-water fish than in the warmer-adapted fish relative to the ETS (complexes I and III) when measured close to the species optimal temperatures. The activity of HOAD showed a similar pattern relative to CI + III and thermal environment. By contrast, PDHc and PK did not show the similar patterns with respect to CI + III and temperature. Cold-adapted species had high CIV activities compared to those of upstream complexes (I, II, I + III) whereas the converse was true for warm-adapted species. Our findings reveal a significant variability of heart mitochondrial organization among species that can be linked to temperature adaptation. Cold-adapted fish do not appear to compensate for PDHc activity but likely adjust fatty acids oxidation through higher activities of CPT and HOAD relative to complexes I + III. |
format |
Still Image |
author |
Florence Hunter-Manseau Véronique Desrosiers Nathalie R. Le François France Dufresne H. William Detrich Christian Nozais Pierre U. Blier |
author_facet |
Florence Hunter-Manseau Véronique Desrosiers Nathalie R. Le François France Dufresne H. William Detrich Christian Nozais Pierre U. Blier |
author_sort |
Florence Hunter-Manseau |
title |
Image_9_From Africa to Antarctica: Exploring the Metabolism of Fish Heart Mitochondria Across a Wide Thermal Range.pdf |
title_short |
Image_9_From Africa to Antarctica: Exploring the Metabolism of Fish Heart Mitochondria Across a Wide Thermal Range.pdf |
title_full |
Image_9_From Africa to Antarctica: Exploring the Metabolism of Fish Heart Mitochondria Across a Wide Thermal Range.pdf |
title_fullStr |
Image_9_From Africa to Antarctica: Exploring the Metabolism of Fish Heart Mitochondria Across a Wide Thermal Range.pdf |
title_full_unstemmed |
Image_9_From Africa to Antarctica: Exploring the Metabolism of Fish Heart Mitochondria Across a Wide Thermal Range.pdf |
title_sort |
image_9_from africa to antarctica: exploring the metabolism of fish heart mitochondria across a wide thermal range.pdf |
publishDate |
2019 |
url |
https://doi.org/10.3389/fphys.2019.01220.s010 https://figshare.com/articles/Image_9_From_Africa_to_Antarctica_Exploring_the_Metabolism_of_Fish_Heart_Mitochondria_Across_a_Wide_Thermal_Range_pdf/9938468 |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_relation |
doi:10.3389/fphys.2019.01220.s010 https://figshare.com/articles/Image_9_From_Africa_to_Antarctica_Exploring_the_Metabolism_of_Fish_Heart_Mitochondria_Across_a_Wide_Thermal_Range_pdf/9938468 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fphys.2019.01220.s010 |
_version_ |
1766272715597021184 |