Image_1_Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.).pdf
The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 10 6 CFU/g). In this context, f...
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Online Access: | https://doi.org/10.3389/fmicb.2019.02243.s001 https://figshare.com/articles/Image_1_Influence_of_Dietary_Supplementation_of_Probiotic_Pediococcus_acidilactici_MA18_5M_During_the_Transition_From_Freshwater_to_Seawater_on_Intestinal_Health_and_Microbiota_of_Atlantic_Salmon_Salmo_salar_L_pdf/9913670 |
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ftfrontimediafig:oai:figshare.com:article/9913670 2023-05-15T15:31:16+02:00 Image_1_Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.).pdf Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield 2019-09-27T12:33:35Z https://doi.org/10.3389/fmicb.2019.02243.s001 https://figshare.com/articles/Image_1_Influence_of_Dietary_Supplementation_of_Probiotic_Pediococcus_acidilactici_MA18_5M_During_the_Transition_From_Freshwater_to_Seawater_on_Intestinal_Health_and_Microbiota_of_Atlantic_Salmon_Salmo_salar_L_pdf/9913670 unknown doi:10.3389/fmicb.2019.02243.s001 https://figshare.com/articles/Image_1_Influence_of_Dietary_Supplementation_of_Probiotic_Pediococcus_acidilactici_MA18_5M_During_the_Transition_From_Freshwater_to_Seawater_on_Intestinal_Health_and_Microbiota_of_Atlantic_Salmon_Salmo_salar_L_pdf/9913670 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing antiviral response Image Figure 2019 ftfrontimediafig https://doi.org/10.3389/fmicb.2019.02243.s001 2019-10-02T22:59:42Z The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 10 6 CFU/g). In this context, fish health and antiviral response were also investigated. A 12-week feeding trial was conducted in a flow-through rearing system involving 6 weeks in freshwater and 6 weeks in seawater. Fish received a control and probiotic diet. The composition of the salmon gut bacterial communities was determined by high-throughput sequencing of digesta and mucosa samples from both the freshwater and seawater stage. The main phyla detected during both freshwater and seawater stages were Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. Significant differences were observed between the intestinal microbiota in the digesta and the mucosa. Both probiotic supplementation and the seawater transfer (SWT) had a substantial impact on the microbial communities, with most pronounced changes detected in the mucosal communities after SWT. This last finding together with a significantly higher antiviral response (mx-1 and tlr3 gene expression) in the distal intestine of fish fed the probiotic diet suggest a causal link between the microbiota modulation and activation of antiviral response. Feeding probiotics during the freshwater stage did not significantly increase survival after infectious pancreatic necrosis virus (IPNV) challenge after SWT, although higher survival was observed in one out of two replicate challenge tanks. In conclusion, this study demonstrated that both dietary probiotic supplementation and transfer from freshwater to seawater have an important role in modulating the bacterial communities in the distal intestine of Atlantic salmon. Furthermore, supplementation of the diet with P. acidilactici MA18/5M can modulate antiviral response. Still Image Atlantic salmon Salmo salar Frontiers: Figshare |
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Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing antiviral response |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing antiviral response Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield Image_1_Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.).pdf |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology fish microbiota intestine Pediococcus acidilactici seawater transfer high-throughput sequencing antiviral response |
description |
The aim of this study was to assess the effect of the transfer from freshwater to seawater on the distal intestinal bacterial communities of Atlantic salmon (Salmo salar L.) and to evaluate the effect of dietary inclusion of Pediococcus acidilactici MA18/5M (at 1.19 × 10 6 CFU/g). In this context, fish health and antiviral response were also investigated. A 12-week feeding trial was conducted in a flow-through rearing system involving 6 weeks in freshwater and 6 weeks in seawater. Fish received a control and probiotic diet. The composition of the salmon gut bacterial communities was determined by high-throughput sequencing of digesta and mucosa samples from both the freshwater and seawater stage. The main phyla detected during both freshwater and seawater stages were Firmicutes, Proteobacteria, Fusobacteria, and Actinobacteria. Significant differences were observed between the intestinal microbiota in the digesta and the mucosa. Both probiotic supplementation and the seawater transfer (SWT) had a substantial impact on the microbial communities, with most pronounced changes detected in the mucosal communities after SWT. This last finding together with a significantly higher antiviral response (mx-1 and tlr3 gene expression) in the distal intestine of fish fed the probiotic diet suggest a causal link between the microbiota modulation and activation of antiviral response. Feeding probiotics during the freshwater stage did not significantly increase survival after infectious pancreatic necrosis virus (IPNV) challenge after SWT, although higher survival was observed in one out of two replicate challenge tanks. In conclusion, this study demonstrated that both dietary probiotic supplementation and transfer from freshwater to seawater have an important role in modulating the bacterial communities in the distal intestine of Atlantic salmon. Furthermore, supplementation of the diet with P. acidilactici MA18/5M can modulate antiviral response. |
format |
Still Image |
author |
Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield |
author_facet |
Alexander Jaramillo-Torres Mark D. Rawling Ana Rodiles Heidi E. Mikalsen Lill-Heidi Johansen John Tinsley Torunn Forberg Elisabeth Aasum Mathieu Castex Daniel Lee Merrifield |
author_sort |
Alexander Jaramillo-Torres |
title |
Image_1_Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.).pdf |
title_short |
Image_1_Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.).pdf |
title_full |
Image_1_Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.).pdf |
title_fullStr |
Image_1_Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.).pdf |
title_full_unstemmed |
Image_1_Influence of Dietary Supplementation of Probiotic Pediococcus acidilactici MA18/5M During the Transition From Freshwater to Seawater on Intestinal Health and Microbiota of Atlantic Salmon (Salmo salar L.).pdf |
title_sort |
image_1_influence of dietary supplementation of probiotic pediococcus acidilactici ma18/5m during the transition from freshwater to seawater on intestinal health and microbiota of atlantic salmon (salmo salar l.).pdf |
publishDate |
2019 |
url |
https://doi.org/10.3389/fmicb.2019.02243.s001 https://figshare.com/articles/Image_1_Influence_of_Dietary_Supplementation_of_Probiotic_Pediococcus_acidilactici_MA18_5M_During_the_Transition_From_Freshwater_to_Seawater_on_Intestinal_Health_and_Microbiota_of_Atlantic_Salmon_Salmo_salar_L_pdf/9913670 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_relation |
doi:10.3389/fmicb.2019.02243.s001 https://figshare.com/articles/Image_1_Influence_of_Dietary_Supplementation_of_Probiotic_Pediococcus_acidilactici_MA18_5M_During_the_Transition_From_Freshwater_to_Seawater_on_Intestinal_Health_and_Microbiota_of_Atlantic_Salmon_Salmo_salar_L_pdf/9913670 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2019.02243.s001 |
_version_ |
1766361757770579968 |