Data_Sheet_1_Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response.docx
IκB kinases (IKKs) play critical roles in innate immunity through signal-induced activation of the key transcription factors nuclear factor-κB (NF-κB) and interferon regulatory factors (IRFs). However, studies of invertebrate IKK functions remain scarce. In this study, we performed phylogenetic anal...
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Online Access: | https://doi.org/10.3389/fimmu.2019.01826.s001 https://figshare.com/articles/Data_Sheet_1_Oyster_Versatile_IKK_s_Are_Involved_in_Toll-Like_Receptor_and_RIG-I-Like_Receptor_Signaling_for_Innate_Immune_Response_docx/9198938 |
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ftfrontimediafig:oai:figshare.com:article/9198938 2023-05-15T15:59:10+02:00 Data_Sheet_1_Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response.docx Baoyu Huang Linlin Zhang Fei Xu Xueying Tang Li Li Wei Wang Mingkun Liu Guofan Zhang 2019-07-31T13:14:37Z https://doi.org/10.3389/fimmu.2019.01826.s001 https://figshare.com/articles/Data_Sheet_1_Oyster_Versatile_IKK_s_Are_Involved_in_Toll-Like_Receptor_and_RIG-I-Like_Receptor_Signaling_for_Innate_Immune_Response_docx/9198938 unknown doi:10.3389/fimmu.2019.01826.s001 https://figshare.com/articles/Data_Sheet_1_Oyster_Versatile_IKK_s_Are_Involved_in_Toll-Like_Receptor_and_RIG-I-Like_Receptor_Signaling_for_Innate_Immune_Response_docx/9198938 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas IKKα IKKβ Toll-like receptor RIG-I-like receptor NF-κB interferon regulatory factor Dataset 2019 ftfrontimediafig https://doi.org/10.3389/fimmu.2019.01826.s001 2019-08-07T23:00:42Z IκB kinases (IKKs) play critical roles in innate immunity through signal-induced activation of the key transcription factors nuclear factor-κB (NF-κB) and interferon regulatory factors (IRFs). However, studies of invertebrate IKK functions remain scarce. In this study, we performed phylogenetic analysis of IKKs and IKK-related kinases encoded in the Pacific oyster genome. We then cloned and characterized the oyster IKKα/β-2 gene. We found that oyster IKKα/β-2, a homolog of human IKKα/IKKβ, responded to challenge with lipopolysaccharide (LPS), peptidoglycan (PGN), and polyinosinic-polycytidylic acid [poly(I:C)]. As a versatile immune molecule, IKKα/β-2 activated the promoters of NF-κB, TNFα, and IFNβ, as well as IFN-stimulated response element (ISRE)-containing promoters, initiating an antibacterial or antiviral immune state in mammalian cells. Importantly, together with the cloned oyster IKKα/β-1, we investigated the signal transduction pathways mediated by these two IKKα/β proteins. Our results showed that IKKα/β-1 and IKKα/β-2 could interact with the oyster TNF receptor-associated factor 6 (TRAF6) and that IKKα/β-2 could also bind to the oyster myeloid differentiation factor 88 (MyD88) protein directly, suggesting that oyster IKKα/βs participate in both RIG-I-like receptor (RLR) and Toll-like receptor (TLR) signaling for the reception of upstream immune signals. The fact that IKKα/β-1 and IKKα/β-2 formed homodimers by interacting with themselves and heterodimers by interacting with each other, along with the fact that both oyster IKKα/β proteins interacted with NEMO protein, indicates that oyster IKKα/βs and the scaffold protein NEMO form an IKK complex, which may be a key step in phosphorylating IκB proteins and activating NF-κB. Moreover, we found that oyster IKKα/βs could interact with IRF8, and this may be related to the IKK-mediated activation of ISRE promotors and their involvement in the oyster “interferon (IFN)-like” antiviral pathway. Moreover, the expression of oyster IKKα/β-1 and IKKα/β-2 may induce ... Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas IKKα IKKβ Toll-like receptor RIG-I-like receptor NF-κB interferon regulatory factor |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas IKKα IKKβ Toll-like receptor RIG-I-like receptor NF-κB interferon regulatory factor Baoyu Huang Linlin Zhang Fei Xu Xueying Tang Li Li Wei Wang Mingkun Liu Guofan Zhang Data_Sheet_1_Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response.docx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas IKKα IKKβ Toll-like receptor RIG-I-like receptor NF-κB interferon regulatory factor |
description |
IκB kinases (IKKs) play critical roles in innate immunity through signal-induced activation of the key transcription factors nuclear factor-κB (NF-κB) and interferon regulatory factors (IRFs). However, studies of invertebrate IKK functions remain scarce. In this study, we performed phylogenetic analysis of IKKs and IKK-related kinases encoded in the Pacific oyster genome. We then cloned and characterized the oyster IKKα/β-2 gene. We found that oyster IKKα/β-2, a homolog of human IKKα/IKKβ, responded to challenge with lipopolysaccharide (LPS), peptidoglycan (PGN), and polyinosinic-polycytidylic acid [poly(I:C)]. As a versatile immune molecule, IKKα/β-2 activated the promoters of NF-κB, TNFα, and IFNβ, as well as IFN-stimulated response element (ISRE)-containing promoters, initiating an antibacterial or antiviral immune state in mammalian cells. Importantly, together with the cloned oyster IKKα/β-1, we investigated the signal transduction pathways mediated by these two IKKα/β proteins. Our results showed that IKKα/β-1 and IKKα/β-2 could interact with the oyster TNF receptor-associated factor 6 (TRAF6) and that IKKα/β-2 could also bind to the oyster myeloid differentiation factor 88 (MyD88) protein directly, suggesting that oyster IKKα/βs participate in both RIG-I-like receptor (RLR) and Toll-like receptor (TLR) signaling for the reception of upstream immune signals. The fact that IKKα/β-1 and IKKα/β-2 formed homodimers by interacting with themselves and heterodimers by interacting with each other, along with the fact that both oyster IKKα/β proteins interacted with NEMO protein, indicates that oyster IKKα/βs and the scaffold protein NEMO form an IKK complex, which may be a key step in phosphorylating IκB proteins and activating NF-κB. Moreover, we found that oyster IKKα/βs could interact with IRF8, and this may be related to the IKK-mediated activation of ISRE promotors and their involvement in the oyster “interferon (IFN)-like” antiviral pathway. Moreover, the expression of oyster IKKα/β-1 and IKKα/β-2 may induce ... |
format |
Dataset |
author |
Baoyu Huang Linlin Zhang Fei Xu Xueying Tang Li Li Wei Wang Mingkun Liu Guofan Zhang |
author_facet |
Baoyu Huang Linlin Zhang Fei Xu Xueying Tang Li Li Wei Wang Mingkun Liu Guofan Zhang |
author_sort |
Baoyu Huang |
title |
Data_Sheet_1_Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response.docx |
title_short |
Data_Sheet_1_Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response.docx |
title_full |
Data_Sheet_1_Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response.docx |
title_fullStr |
Data_Sheet_1_Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response.docx |
title_full_unstemmed |
Data_Sheet_1_Oyster Versatile IKKα/βs Are Involved in Toll-Like Receptor and RIG-I-Like Receptor Signaling for Innate Immune Response.docx |
title_sort |
data_sheet_1_oyster versatile ikkα/βs are involved in toll-like receptor and rig-i-like receptor signaling for innate immune response.docx |
publishDate |
2019 |
url |
https://doi.org/10.3389/fimmu.2019.01826.s001 https://figshare.com/articles/Data_Sheet_1_Oyster_Versatile_IKK_s_Are_Involved_in_Toll-Like_Receptor_and_RIG-I-Like_Receptor_Signaling_for_Innate_Immune_Response_docx/9198938 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2019.01826.s001 https://figshare.com/articles/Data_Sheet_1_Oyster_Versatile_IKK_s_Are_Involved_in_Toll-Like_Receptor_and_RIG-I-Like_Receptor_Signaling_for_Innate_Immune_Response_docx/9198938 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2019.01826.s001 |
_version_ |
1766394952529477632 |