Table_1_Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures.xlsx

Climate change is causing arctic regions to warm disproportionally faster than those at lower latitudes, leading to alterations in carbon and nitrogen cycling, and potentially higher greenhouse gas emissions. It is thus increasingly important to better characterize the microorganisms driving arctic...

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Main Authors: Ricardo J. Eloy Alves, Melina Kerou, Anna Zappe, Romana Bittner, Sophie S. Abby, Heiko A. Schmidt, Kevin Pfeifer, Christa Schleper
Format: Dataset
Language:unknown
Published: 2019
Subjects:
Online Access:https://doi.org/10.3389/fmicb.2019.01571.s002
https://figshare.com/articles/Table_1_Ammonia_Oxidation_by_the_Arctic_Terrestrial_Thaumarchaeote_Candidatus_Nitrosocosmicus_arcticus_Is_Stimulated_by_Increasing_Temperatures_xlsx/8952830
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spelling ftfrontimediafig:oai:figshare.com:article/8952830 2023-05-15T14:38:44+02:00 Table_1_Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures.xlsx Ricardo J. Eloy Alves Melina Kerou Anna Zappe Romana Bittner Sophie S. Abby Heiko A. Schmidt Kevin Pfeifer Christa Schleper 2019-07-18T13:56:43Z https://doi.org/10.3389/fmicb.2019.01571.s002 https://figshare.com/articles/Table_1_Ammonia_Oxidation_by_the_Arctic_Terrestrial_Thaumarchaeote_Candidatus_Nitrosocosmicus_arcticus_Is_Stimulated_by_Increasing_Temperatures_xlsx/8952830 unknown doi:10.3389/fmicb.2019.01571.s002 https://figshare.com/articles/Table_1_Ammonia_Oxidation_by_the_Arctic_Terrestrial_Thaumarchaeote_Candidatus_Nitrosocosmicus_arcticus_Is_Stimulated_by_Increasing_Temperatures_xlsx/8952830 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology ammonia oxidation archaea thaumarchaeota nitrification arctic ecosystems soil microbiology Dataset 2019 ftfrontimediafig https://doi.org/10.3389/fmicb.2019.01571.s002 2019-08-07T23:03:32Z Climate change is causing arctic regions to warm disproportionally faster than those at lower latitudes, leading to alterations in carbon and nitrogen cycling, and potentially higher greenhouse gas emissions. It is thus increasingly important to better characterize the microorganisms driving arctic biogeochemical processes and their potential responses to changing conditions. Here, we describe a novel thaumarchaeon enriched from an arctic soil, Candidatus Nitrosocosmicus arcticus strain Kfb, which has been maintained for seven years in stable laboratory enrichment cultures as an aerobic ammonia oxidizer, with ammonium or urea as substrates. Genomic analyses show that this organism harbors all genes involved in ammonia oxidation and in carbon fixation via the 3-hydroxypropionate/4-hydroxybutyrate cycle, characteristic of all AOA, as well as the capability for urea utilization and potentially also for heterotrophic metabolism, similar to other AOA. Ca. N. arcticus oxidizes ammonia optimally between 20 and 28°C, well above average temperatures in its native high arctic environment (−13–4°C). Ammonia oxidation rates were nevertheless much lower than those of most cultivated mesophilic AOA (20–45°C). Intriguingly, we repeatedly observed apparent faster growth rates (based on marker gene counts) at lower temperatures (4–8°C) but without detectable nitrite production. Together with potential metabolisms predicted from its genome content, these observations indicate that Ca. N. arcticus is not a strict chemolithotrophic ammonia oxidizer and add to cumulating evidence for a greater metabolic and physiological versatility of AOA. The physiology of Ca. N. arcticus suggests that increasing temperatures might drastically affect nitrification in arctic soils by stimulating archaeal ammonia oxidation. Dataset Arctic Climate change Frontiers: Figshare Arctic
institution Open Polar
collection Frontiers: Figshare
op_collection_id ftfrontimediafig
language unknown
topic Microbiology
Microbial Genetics
Microbial Ecology
Mycology
ammonia oxidation
archaea
thaumarchaeota
nitrification
arctic ecosystems
soil microbiology
spellingShingle Microbiology
Microbial Genetics
Microbial Ecology
Mycology
ammonia oxidation
archaea
thaumarchaeota
nitrification
arctic ecosystems
soil microbiology
Ricardo J. Eloy Alves
Melina Kerou
Anna Zappe
Romana Bittner
Sophie S. Abby
Heiko A. Schmidt
Kevin Pfeifer
Christa Schleper
Table_1_Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures.xlsx
topic_facet Microbiology
Microbial Genetics
Microbial Ecology
Mycology
ammonia oxidation
archaea
thaumarchaeota
nitrification
arctic ecosystems
soil microbiology
description Climate change is causing arctic regions to warm disproportionally faster than those at lower latitudes, leading to alterations in carbon and nitrogen cycling, and potentially higher greenhouse gas emissions. It is thus increasingly important to better characterize the microorganisms driving arctic biogeochemical processes and their potential responses to changing conditions. Here, we describe a novel thaumarchaeon enriched from an arctic soil, Candidatus Nitrosocosmicus arcticus strain Kfb, which has been maintained for seven years in stable laboratory enrichment cultures as an aerobic ammonia oxidizer, with ammonium or urea as substrates. Genomic analyses show that this organism harbors all genes involved in ammonia oxidation and in carbon fixation via the 3-hydroxypropionate/4-hydroxybutyrate cycle, characteristic of all AOA, as well as the capability for urea utilization and potentially also for heterotrophic metabolism, similar to other AOA. Ca. N. arcticus oxidizes ammonia optimally between 20 and 28°C, well above average temperatures in its native high arctic environment (−13–4°C). Ammonia oxidation rates were nevertheless much lower than those of most cultivated mesophilic AOA (20–45°C). Intriguingly, we repeatedly observed apparent faster growth rates (based on marker gene counts) at lower temperatures (4–8°C) but without detectable nitrite production. Together with potential metabolisms predicted from its genome content, these observations indicate that Ca. N. arcticus is not a strict chemolithotrophic ammonia oxidizer and add to cumulating evidence for a greater metabolic and physiological versatility of AOA. The physiology of Ca. N. arcticus suggests that increasing temperatures might drastically affect nitrification in arctic soils by stimulating archaeal ammonia oxidation.
format Dataset
author Ricardo J. Eloy Alves
Melina Kerou
Anna Zappe
Romana Bittner
Sophie S. Abby
Heiko A. Schmidt
Kevin Pfeifer
Christa Schleper
author_facet Ricardo J. Eloy Alves
Melina Kerou
Anna Zappe
Romana Bittner
Sophie S. Abby
Heiko A. Schmidt
Kevin Pfeifer
Christa Schleper
author_sort Ricardo J. Eloy Alves
title Table_1_Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures.xlsx
title_short Table_1_Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures.xlsx
title_full Table_1_Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures.xlsx
title_fullStr Table_1_Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures.xlsx
title_full_unstemmed Table_1_Ammonia Oxidation by the Arctic Terrestrial Thaumarchaeote Candidatus Nitrosocosmicus arcticus Is Stimulated by Increasing Temperatures.xlsx
title_sort table_1_ammonia oxidation by the arctic terrestrial thaumarchaeote candidatus nitrosocosmicus arcticus is stimulated by increasing temperatures.xlsx
publishDate 2019
url https://doi.org/10.3389/fmicb.2019.01571.s002
https://figshare.com/articles/Table_1_Ammonia_Oxidation_by_the_Arctic_Terrestrial_Thaumarchaeote_Candidatus_Nitrosocosmicus_arcticus_Is_Stimulated_by_Increasing_Temperatures_xlsx/8952830
geographic Arctic
geographic_facet Arctic
genre Arctic
Climate change
genre_facet Arctic
Climate change
op_relation doi:10.3389/fmicb.2019.01571.s002
https://figshare.com/articles/Table_1_Ammonia_Oxidation_by_the_Arctic_Terrestrial_Thaumarchaeote_Candidatus_Nitrosocosmicus_arcticus_Is_Stimulated_by_Increasing_Temperatures_xlsx/8952830
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fmicb.2019.01571.s002
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