Table_2_Highly Diverse Aquatic Microbial Communities Separated by Permafrost in Greenland Show Distinct Features According to Environmental Niches.xlsx

• Main Authors: Malin Bomberg, Lillemor Claesson Liljedahl, Tiina Lamminmäki, Anne Kontula
• Format: Dataset
• Language: unknown
• Published: 2019
• Subjects: Microbiology; Microbial Genetics; Microbial Ecology; Mycology; deep biosphere; nitrogen fixation; iron reduction; photosynthesis; groundwater; metabolic profile; Greenland; Pond Lake; Talik; glacier; Ice; permafrost
• Online Access: https://doi.org/10.3389/fmicb.2019.01583.s003; https://figshare.com/articles/Table_2_Highly_Diverse_Aquatic_Microbial_Communities_Separated_by_Permafrost_in_Greenland_Show_Distinct_Features_According_to_Environmental_Niches_xlsx/8858744

The Greenland Analog Project (GAP) study area in the vicinity of Kangarlussuaq, Western Greenland, was sampled for surface water and deep groundwater in order to determine the composition and estimate the metabolic features of the microbial communities in water bodies separated by permafrost. The sampling sites comprised a freshwater pond, talik lake, deep anoxic groundwater, glacier ice and supraglacial river, meltwater river and melting permafrost active layer. The microbial communities were characterized by amplicon sequencing of the bacterial and archaeal 16S rRNA genes and fungal ITS1 spacer. In addition, bacterial, archaeal and fungal numbers were determined by qPCR and plate counts, and the utilization pattern of carbon and nitrogen substrates was determined with Biolog AN plates and metabolic functions were predicted with FAPROTAX. Different sample types were clearly distinguishable from each other based on community composition, microbial numbers, and substrate utilization patterns, forming four groups, (1) pond/lake, (2) deep groundwater, (3) glacial ice, and (4) meltwater. Bacteria were the most abundant microbial domain, ranging from 0.2–1.4 × 10 7 16S rRNA gene copies mL -1 in pond/lake and meltwater, 0.1-7.8 × 10 6 copies mL -1 in groundwater and less than 10 4 copies mL -1 in ice. The number of archaeal 16S and fungal 5.8S rRNA genes was generally less than 6.0 × 10 3 and 1.5 × 10 3 , respectively. N 2 -fixing and methane-oxidizing Actinomycetes, Bacteroidetes and Verrucomicrobia were the dominant microorganisms in the pond/lake samples, whereas iron reducing Desulfosporosinus sp. dominated the deep anaerobic groundwater. The glacial ice was inhabited by Cyanobacteria, which were mostly Chloroplast-like. The meltwater contained methano- and methylotrophic Proteobacteria, but had also high relative abundances of the nano-sized Parcubacteria. The archaea composed approximately 1% of the 16S rRNA gene pool in the pond/lake samples with nano-sized Woesearchaeota as the dominating taxon, while in the ...