Data_Sheet_1_Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages.docx
In contrast to other teleosts, Atlantic cod (Gadus morhua) has an expanded repertoire of MHC-I and TLR components, but lacks the MHC-II, the invariant chain/CD74, and CD4 + T cell response, essential for production of antibodies and prevention of bacterial infectious diseases. The mechanisms by whic...
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ftfrontimediafig:oai:figshare.com:article/8224457 2023-05-15T15:26:39+02:00 Data_Sheet_1_Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages.docx Manuel Soto-Dávila Ahmed Hossain Setu Chakraborty Matthew L. Rise Javier Santander 2019-06-04T15:14:09Z https://doi.org/10.3389/fimmu.2019.01237.s001 https://figshare.com/articles/Data_Sheet_1_Aeromonas_salmonicida_subsp_salmonicida_Early_Infection_and_Immune_Response_of_Atlantic_Cod_Gadus_morhua_L_Primary_Macrophages_docx/8224457 unknown doi:10.3389/fimmu.2019.01237.s001 https://figshare.com/articles/Data_Sheet_1_Aeromonas_salmonicida_subsp_salmonicida_Early_Infection_and_Immune_Response_of_Atlantic_Cod_Gadus_morhua_L_Primary_Macrophages_docx/8224457 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic cod Gadiform primary macrophages Aeromonas salmonicida Gram-negative Dataset 2019 ftfrontimediafig https://doi.org/10.3389/fimmu.2019.01237.s001 2019-06-05T22:58:06Z In contrast to other teleosts, Atlantic cod (Gadus morhua) has an expanded repertoire of MHC-I and TLR components, but lacks the MHC-II, the invariant chain/CD74, and CD4 + T cell response, essential for production of antibodies and prevention of bacterial infectious diseases. The mechanisms by which G. morhua fight bacterial infections are not well understood. Aeromonas salmonicida subsp. salmonicida is a recurrent pathogen in cultured and wild fish, and has been reported in Atlantic cod. Macrophages are some of the first responders to bacterial infection and the link between innate and adaptive immune response. Here, we evaluated the viability, reactive oxygen species (ROS) production, cell morphology, and gene expression of cod primary macrophages in response to A. salmonicida infection. We found that A. salmonicida infects cod primary macrophages without killing the cod cells. Likewise, infected Atlantic cod macrophages up-regulated key genes involved in the inflammatory response (e.g., IL-1β and IL-8) and bacterial recognition (e.g., BPI/LBP). Nevertheless, our results showed a down-regulation of genes related to antimicrobial peptide and ROS production, suggesting that A. salmonicida utilizes its virulence mechanisms to control and prevent macrophage anti-bacterial activity. Our results also indicate that Atlantic cod has a basal ROS production in non-infected cells, and this was not increased after contact with A. salmonicida. Transmission electron microscopy results showed that A. salmonicida was able to infect the macrophages in a high number, and release outer membrane vesicles (OMV) during intracellular infection. These results suggest that Atlantic cod macrophage innate immunity is able to detect A. salmonicida and trigger an anti-inflammatory response, however A. salmonicida controls the cell immune response to prevent bacterial clearance, during early infection. Dataset atlantic cod Gadus morhua Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic cod Gadiform primary macrophages Aeromonas salmonicida Gram-negative |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic cod Gadiform primary macrophages Aeromonas salmonicida Gram-negative Manuel Soto-Dávila Ahmed Hossain Setu Chakraborty Matthew L. Rise Javier Santander Data_Sheet_1_Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages.docx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Atlantic cod Gadiform primary macrophages Aeromonas salmonicida Gram-negative |
description |
In contrast to other teleosts, Atlantic cod (Gadus morhua) has an expanded repertoire of MHC-I and TLR components, but lacks the MHC-II, the invariant chain/CD74, and CD4 + T cell response, essential for production of antibodies and prevention of bacterial infectious diseases. The mechanisms by which G. morhua fight bacterial infections are not well understood. Aeromonas salmonicida subsp. salmonicida is a recurrent pathogen in cultured and wild fish, and has been reported in Atlantic cod. Macrophages are some of the first responders to bacterial infection and the link between innate and adaptive immune response. Here, we evaluated the viability, reactive oxygen species (ROS) production, cell morphology, and gene expression of cod primary macrophages in response to A. salmonicida infection. We found that A. salmonicida infects cod primary macrophages without killing the cod cells. Likewise, infected Atlantic cod macrophages up-regulated key genes involved in the inflammatory response (e.g., IL-1β and IL-8) and bacterial recognition (e.g., BPI/LBP). Nevertheless, our results showed a down-regulation of genes related to antimicrobial peptide and ROS production, suggesting that A. salmonicida utilizes its virulence mechanisms to control and prevent macrophage anti-bacterial activity. Our results also indicate that Atlantic cod has a basal ROS production in non-infected cells, and this was not increased after contact with A. salmonicida. Transmission electron microscopy results showed that A. salmonicida was able to infect the macrophages in a high number, and release outer membrane vesicles (OMV) during intracellular infection. These results suggest that Atlantic cod macrophage innate immunity is able to detect A. salmonicida and trigger an anti-inflammatory response, however A. salmonicida controls the cell immune response to prevent bacterial clearance, during early infection. |
format |
Dataset |
author |
Manuel Soto-Dávila Ahmed Hossain Setu Chakraborty Matthew L. Rise Javier Santander |
author_facet |
Manuel Soto-Dávila Ahmed Hossain Setu Chakraborty Matthew L. Rise Javier Santander |
author_sort |
Manuel Soto-Dávila |
title |
Data_Sheet_1_Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages.docx |
title_short |
Data_Sheet_1_Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages.docx |
title_full |
Data_Sheet_1_Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages.docx |
title_fullStr |
Data_Sheet_1_Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages.docx |
title_full_unstemmed |
Data_Sheet_1_Aeromonas salmonicida subsp. salmonicida Early Infection and Immune Response of Atlantic Cod (Gadus morhua L.) Primary Macrophages.docx |
title_sort |
data_sheet_1_aeromonas salmonicida subsp. salmonicida early infection and immune response of atlantic cod (gadus morhua l.) primary macrophages.docx |
publishDate |
2019 |
url |
https://doi.org/10.3389/fimmu.2019.01237.s001 https://figshare.com/articles/Data_Sheet_1_Aeromonas_salmonicida_subsp_salmonicida_Early_Infection_and_Immune_Response_of_Atlantic_Cod_Gadus_morhua_L_Primary_Macrophages_docx/8224457 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
doi:10.3389/fimmu.2019.01237.s001 https://figshare.com/articles/Data_Sheet_1_Aeromonas_salmonicida_subsp_salmonicida_Early_Infection_and_Immune_Response_of_Atlantic_Cod_Gadus_morhua_L_Primary_Macrophages_docx/8224457 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2019.01237.s001 |
_version_ |
1766357125331681280 |