Table_1_Dryas as a Model for Studying the Root Symbioses of the Rosaceae.XLSX
The nitrogen-fixing root nodule symbiosis is restricted to four plant orders: Fabales (legumes), Fagales, Cucurbitales and Rosales (Elaeagnaceae, Rhamnaceae, and Rosaceae). Interestingly all of the Rosaceae genera confirmed to contain nodulating species (i.e., Cercocarpus, Chamaebatia, Dryas, and Pu...
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ftfrontimediafig:oai:figshare.com:article/8221775 2023-05-15T16:02:43+02:00 Table_1_Dryas as a Model for Studying the Root Symbioses of the Rosaceae.XLSX Benjamin Billault-Penneteau Aline Sandré Jessica Folgmann Martin Parniske Katharina Pawlowski 2019-06-04T05:19:08Z https://doi.org/10.3389/fpls.2019.00661.s001 https://figshare.com/articles/Table_1_Dryas_as_a_Model_for_Studying_the_Root_Symbioses_of_the_Rosaceae_XLSX/8221775 unknown doi:10.3389/fpls.2019.00661.s001 https://figshare.com/articles/Table_1_Dryas_as_a_Model_for_Studying_the_Root_Symbioses_of_the_Rosaceae_XLSX/8221775 CC BY 4.0 CC-BY Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified Dryas model-plant Dryas drummondii Dryas octopetala Rosaceae genome comparison Dataset 2019 ftfrontimediafig https://doi.org/10.3389/fpls.2019.00661.s001 2019-06-05T22:58:19Z The nitrogen-fixing root nodule symbiosis is restricted to four plant orders: Fabales (legumes), Fagales, Cucurbitales and Rosales (Elaeagnaceae, Rhamnaceae, and Rosaceae). Interestingly all of the Rosaceae genera confirmed to contain nodulating species (i.e., Cercocarpus, Chamaebatia, Dryas, and Purshia) belong to a single subfamily, the Dryadoideae. The Dryas genus is particularly interesting from an evolutionary perspective because it contains closely related nodulating (Dryas drummondii) and non-nodulating species (Dryas octopetala). The close phylogenetic relationship between these two species makes Dryas an ideal model genus to study the genetic basis of nodulation by whole genome comparison and classical genetics. Therefore, we established methods for plant cultivation, transformation and DNA extraction for these species. We optimized seed surface sterilization and germination methods and tested growth protocols ranging from pots and Petri dishes to a hydroponic system. Transgenic hairy roots were obtained by adapting Agrobacterium rhizogenes-based transformation protocols for Dryas species. We compared several DNA extraction protocols for their suitability for subsequent molecular biological analysis. Using CTAB extraction, reproducible PCRs could be performed, but CsCl gradient purification was essential to obtain DNA in sufficient purity for high quality de novo genome sequencing of both Dryas species. Altogether, we established a basic toolkit for the culture, transient transformation and genetic analysis of Dryas sp. Dataset Dryas octopetala Frontiers: Figshare Rosales ENVELOPE(-59.867,-59.867,-62.600,-62.600) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified Dryas model-plant Dryas drummondii Dryas octopetala Rosaceae genome comparison |
spellingShingle |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified Dryas model-plant Dryas drummondii Dryas octopetala Rosaceae genome comparison Benjamin Billault-Penneteau Aline Sandré Jessica Folgmann Martin Parniske Katharina Pawlowski Table_1_Dryas as a Model for Studying the Root Symbioses of the Rosaceae.XLSX |
topic_facet |
Botany Plant Biology Plant Systematics and Taxonomy Plant Cell and Molecular Biology Plant Developmental and Reproductive Biology Plant Pathology Plant Physiology Plant Biology not elsewhere classified Dryas model-plant Dryas drummondii Dryas octopetala Rosaceae genome comparison |
description |
The nitrogen-fixing root nodule symbiosis is restricted to four plant orders: Fabales (legumes), Fagales, Cucurbitales and Rosales (Elaeagnaceae, Rhamnaceae, and Rosaceae). Interestingly all of the Rosaceae genera confirmed to contain nodulating species (i.e., Cercocarpus, Chamaebatia, Dryas, and Purshia) belong to a single subfamily, the Dryadoideae. The Dryas genus is particularly interesting from an evolutionary perspective because it contains closely related nodulating (Dryas drummondii) and non-nodulating species (Dryas octopetala). The close phylogenetic relationship between these two species makes Dryas an ideal model genus to study the genetic basis of nodulation by whole genome comparison and classical genetics. Therefore, we established methods for plant cultivation, transformation and DNA extraction for these species. We optimized seed surface sterilization and germination methods and tested growth protocols ranging from pots and Petri dishes to a hydroponic system. Transgenic hairy roots were obtained by adapting Agrobacterium rhizogenes-based transformation protocols for Dryas species. We compared several DNA extraction protocols for their suitability for subsequent molecular biological analysis. Using CTAB extraction, reproducible PCRs could be performed, but CsCl gradient purification was essential to obtain DNA in sufficient purity for high quality de novo genome sequencing of both Dryas species. Altogether, we established a basic toolkit for the culture, transient transformation and genetic analysis of Dryas sp. |
format |
Dataset |
author |
Benjamin Billault-Penneteau Aline Sandré Jessica Folgmann Martin Parniske Katharina Pawlowski |
author_facet |
Benjamin Billault-Penneteau Aline Sandré Jessica Folgmann Martin Parniske Katharina Pawlowski |
author_sort |
Benjamin Billault-Penneteau |
title |
Table_1_Dryas as a Model for Studying the Root Symbioses of the Rosaceae.XLSX |
title_short |
Table_1_Dryas as a Model for Studying the Root Symbioses of the Rosaceae.XLSX |
title_full |
Table_1_Dryas as a Model for Studying the Root Symbioses of the Rosaceae.XLSX |
title_fullStr |
Table_1_Dryas as a Model for Studying the Root Symbioses of the Rosaceae.XLSX |
title_full_unstemmed |
Table_1_Dryas as a Model for Studying the Root Symbioses of the Rosaceae.XLSX |
title_sort |
table_1_dryas as a model for studying the root symbioses of the rosaceae.xlsx |
publishDate |
2019 |
url |
https://doi.org/10.3389/fpls.2019.00661.s001 https://figshare.com/articles/Table_1_Dryas_as_a_Model_for_Studying_the_Root_Symbioses_of_the_Rosaceae_XLSX/8221775 |
long_lat |
ENVELOPE(-59.867,-59.867,-62.600,-62.600) |
geographic |
Rosales |
geographic_facet |
Rosales |
genre |
Dryas octopetala |
genre_facet |
Dryas octopetala |
op_relation |
doi:10.3389/fpls.2019.00661.s001 https://figshare.com/articles/Table_1_Dryas_as_a_Model_for_Studying_the_Root_Symbioses_of_the_Rosaceae_XLSX/8221775 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fpls.2019.00661.s001 |
_version_ |
1766398396614049792 |