Data_Sheet_1_Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.pdf
Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with the development of High Throughput Sequencing (HTS) techniques. However, some of the diversity remains inaccessible by the power of sequencing. In this study, we combine cult...
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ftfrontimediafig:oai:figshare.com:article/8209871 2023-05-15T13:36:53+02:00 Data_Sheet_1_Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.pdf Adriana Rego Francisco Raio Teresa P. Martins Hugo Ribeiro António G. G. Sousa Joana Séneca Mafalda S. Baptista Charles K. Lee S. Craig Cary Vitor Ramos Maria F. Carvalho Pedro N. Leão Catarina Magalhães 2019-05-31T14:29:13Z https://doi.org/10.3389/fmicb.2019.01018.s001 https://figshare.com/articles/Data_Sheet_1_Actinobacteria_and_Cyanobacteria_Diversity_in_Terrestrial_Antarctic_Microenvironments_Evaluated_by_Culture-Dependent_and_Independent_Methods_pdf/8209871 unknown doi:10.3389/fmicb.2019.01018.s001 https://figshare.com/articles/Data_Sheet_1_Actinobacteria_and_Cyanobacteria_Diversity_in_Terrestrial_Antarctic_Microenvironments_Evaluated_by_Culture-Dependent_and_Independent_Methods_pdf/8209871 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology actinobacteria McMurdo Dry Valleys Antarctic soil bacteria diversity bacterial cultivability endolitic microbiota Antarctic microenvironments cyanobacteria Dataset 2019 ftfrontimediafig https://doi.org/10.3389/fmicb.2019.01018.s001 2019-06-05T22:58:49Z Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with the development of High Throughput Sequencing (HTS) techniques. However, some of the diversity remains inaccessible by the power of sequencing. In this study, we combine cultivation and HTS techniques to survey actinobacteria and cyanobacteria diversity along different soil and endolithic micro-environments of Victoria Valley in McMurdo Dry Valleys. Our results demonstrate that the Dry Valleys actinobacteria and cyanobacteria distribution is driven by environmental forces, in particular the effect of water availability and endolithic environments clearly conditioned the distribution of those communities. Data derived from HTS show that the percentage of cyanobacteria decreases from about 20% in the sample closest to the water source to negligible values on the last three samples of the transect with less water availability. Inversely, actinobacteria relative abundance increases from about 20% in wet soils to over 50% in the driest samples. Over 30% of the total HTS data set was composed of actinobacterial strains, mainly distributed by 5 families: Sporichthyaceae, Euzebyaceae, Patulibacteraceae, Nocardioidaceae, and Rubrobacteraceae. However, the 11 actinobacterial strains isolated in this study, belonged to Micrococcaceae and Dermacoccaceae families that were underrepresented in the HTS data set. A total of 10 cyanobacterial strains from the order Synechococcales were also isolated, distributed by 4 different genera (Nodosilinea, Leptolyngbya, Pectolyngbya, and Acaryochloris-like). In agreement with the cultivation results, Leptolyngbya was identified as dominant genus in the HTS data set. Acaryochloris-like cyanobacteria were found exclusively in the endolithic sample and represented 44% of the total 16S rRNA sequences, although despite our efforts we were not able to properly isolate any strain from this Acaryochloris-related group. The importance of combining cultivation and ... Dataset Antarc* Antarctic Antarctica McMurdo Dry Valleys Frontiers: Figshare Antarctic McMurdo Dry Valleys Victoria Valley ENVELOPE(162.000,162.000,-77.383,-77.383) |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology actinobacteria McMurdo Dry Valleys Antarctic soil bacteria diversity bacterial cultivability endolitic microbiota Antarctic microenvironments cyanobacteria |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology actinobacteria McMurdo Dry Valleys Antarctic soil bacteria diversity bacterial cultivability endolitic microbiota Antarctic microenvironments cyanobacteria Adriana Rego Francisco Raio Teresa P. Martins Hugo Ribeiro António G. G. Sousa Joana Séneca Mafalda S. Baptista Charles K. Lee S. Craig Cary Vitor Ramos Maria F. Carvalho Pedro N. Leão Catarina Magalhães Data_Sheet_1_Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.pdf |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology actinobacteria McMurdo Dry Valleys Antarctic soil bacteria diversity bacterial cultivability endolitic microbiota Antarctic microenvironments cyanobacteria |
description |
Bacterial diversity from McMurdo Dry Valleys in Antarctica, the coldest desert on earth, has become more easily assessed with the development of High Throughput Sequencing (HTS) techniques. However, some of the diversity remains inaccessible by the power of sequencing. In this study, we combine cultivation and HTS techniques to survey actinobacteria and cyanobacteria diversity along different soil and endolithic micro-environments of Victoria Valley in McMurdo Dry Valleys. Our results demonstrate that the Dry Valleys actinobacteria and cyanobacteria distribution is driven by environmental forces, in particular the effect of water availability and endolithic environments clearly conditioned the distribution of those communities. Data derived from HTS show that the percentage of cyanobacteria decreases from about 20% in the sample closest to the water source to negligible values on the last three samples of the transect with less water availability. Inversely, actinobacteria relative abundance increases from about 20% in wet soils to over 50% in the driest samples. Over 30% of the total HTS data set was composed of actinobacterial strains, mainly distributed by 5 families: Sporichthyaceae, Euzebyaceae, Patulibacteraceae, Nocardioidaceae, and Rubrobacteraceae. However, the 11 actinobacterial strains isolated in this study, belonged to Micrococcaceae and Dermacoccaceae families that were underrepresented in the HTS data set. A total of 10 cyanobacterial strains from the order Synechococcales were also isolated, distributed by 4 different genera (Nodosilinea, Leptolyngbya, Pectolyngbya, and Acaryochloris-like). In agreement with the cultivation results, Leptolyngbya was identified as dominant genus in the HTS data set. Acaryochloris-like cyanobacteria were found exclusively in the endolithic sample and represented 44% of the total 16S rRNA sequences, although despite our efforts we were not able to properly isolate any strain from this Acaryochloris-related group. The importance of combining cultivation and ... |
format |
Dataset |
author |
Adriana Rego Francisco Raio Teresa P. Martins Hugo Ribeiro António G. G. Sousa Joana Séneca Mafalda S. Baptista Charles K. Lee S. Craig Cary Vitor Ramos Maria F. Carvalho Pedro N. Leão Catarina Magalhães |
author_facet |
Adriana Rego Francisco Raio Teresa P. Martins Hugo Ribeiro António G. G. Sousa Joana Séneca Mafalda S. Baptista Charles K. Lee S. Craig Cary Vitor Ramos Maria F. Carvalho Pedro N. Leão Catarina Magalhães |
author_sort |
Adriana Rego |
title |
Data_Sheet_1_Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.pdf |
title_short |
Data_Sheet_1_Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.pdf |
title_full |
Data_Sheet_1_Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.pdf |
title_fullStr |
Data_Sheet_1_Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.pdf |
title_full_unstemmed |
Data_Sheet_1_Actinobacteria and Cyanobacteria Diversity in Terrestrial Antarctic Microenvironments Evaluated by Culture-Dependent and Independent Methods.pdf |
title_sort |
data_sheet_1_actinobacteria and cyanobacteria diversity in terrestrial antarctic microenvironments evaluated by culture-dependent and independent methods.pdf |
publishDate |
2019 |
url |
https://doi.org/10.3389/fmicb.2019.01018.s001 https://figshare.com/articles/Data_Sheet_1_Actinobacteria_and_Cyanobacteria_Diversity_in_Terrestrial_Antarctic_Microenvironments_Evaluated_by_Culture-Dependent_and_Independent_Methods_pdf/8209871 |
long_lat |
ENVELOPE(162.000,162.000,-77.383,-77.383) |
geographic |
Antarctic McMurdo Dry Valleys Victoria Valley |
geographic_facet |
Antarctic McMurdo Dry Valleys Victoria Valley |
genre |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
genre_facet |
Antarc* Antarctic Antarctica McMurdo Dry Valleys |
op_relation |
doi:10.3389/fmicb.2019.01018.s001 https://figshare.com/articles/Data_Sheet_1_Actinobacteria_and_Cyanobacteria_Diversity_in_Terrestrial_Antarctic_Microenvironments_Evaluated_by_Culture-Dependent_and_Independent_Methods_pdf/8209871 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2019.01018.s001 |
_version_ |
1766085303029727232 |