Data_Sheet_1_Total and Potentially Active Bacterial Communities Entrapped in a Late Glacial Through Holocene Ice Core From Scarisoara Ice Cave, Romania.PDF

• Main Authors: Victoria I. Paun, Gonzalo Icaza, Paris Lavin, Constantin Marin, Alin Tudorache, Aurel Perşoiu, Cristina Dorador, Cristina Purcarea
• Format: Dataset
• Language: unknown
• Published: 2019
• Subjects: Microbiology; Microbial Genetics; Microbial Ecology; Mycology; bacterial diversity; geochemical composition; Illumina sequencing; perennial cave ice; potentially active microbiome; ice core
• Online Access: https://doi.org/10.3389/fmicb.2019.01193.s001; https://figshare.com/articles/Data_Sheet_1_Total_and_Potentially_Active_Bacterial_Communities_Entrapped_in_a_Late_Glacial_Through_Holocene_Ice_Core_From_Scarisoara_Ice_Cave_Romania_PDF/8197988

Our understanding of the icy-habitat microbiome is likely limited by a lack of reliable data on microorganisms inhabiting underground ice that has accumulated inside caves. To characterize how environmental variation impacts cave ice microbial community structure, we determined the composition of total and potentially active bacterial communities along a 13,000-year-old ice core from Scarisoara cave (Romania) through 16S rRNA gene Illumina sequencing. An average of 2,546 prokaryotic gDNA operational taxonomic units (OTUs) and 585 cDNA OTUs were identified across the perennial cave ice block and analyzed in relation to the geochemical composition of ice layers. The total microbial community and the putative active fraction displayed dissimilar taxa profiles. The ice-contained microbiome was dominated by Actinobacteria with a variable representation of Proteobacteria, while the putative active microbial community was equally shared between Proteobacteria and Firmicutes. Accordingly, a major presence of Cryobacterium, Lysinomonas, Pedobacter, and Aeromicrobium phylotypes homologous to psychrotrophic and psychrophilic bacteria from various cold environments were noted in the total community, while the prevalent putative active bacteria belonged to Clostridium, Pseudomonas, Janthinobacterium, Stenotrophomonas, and Massilia genera. Variation in the microbial cell density of ice strata with the dissolved organic carbon (DOC) content and the strong correlation of DOC and silicon concentrations revealed a major impact of depositional processes on microbial abundance throughout the ice block. Post-depositional processes appeared to occur mostly during the 4,000–7,000 years BP interval. A major bacterial composition shift was observed in 4,500–5,000-year-old ice, leading to a high representation of Beta- and Deltaproteobacteria in the potentially active community in response to the increased concentrations of DOC and major chemical elements. Estimated metabolic rates suggested the presence of a viable microbial community ...