Data_Sheet_1_Modeling the Transmission of Vibrio aestuarianus in Pacific Oysters Using Experimental Infection Data.docx

• Main Authors: Coralie Lupo, Marie-Agnès Travers, Delphine Tourbiez, Clément Félix Barthélémy, Gaël Beaunée, Pauline Ezanno
• Format: Dataset
• Language: unknown
• Published: 2019
• Subjects: Animal Systematics and Taxonomy; Animal Physiology - Biophysics; Animal Physiology - Cell; Animal Physiology - Systems; Animal Behaviour; Animal Cell and Molecular Biology; Animal Developmental and Reproductive Biology; Animal Immunology; Animal Neurobiology; Animal Physiological Ecology; Animal Structure and Function; Veterinary Anaesthesiology and Intensive Care; Veterinary Anatomy and Physiology; Veterinary Diagnosis and Diagnostics; Veterinary Epidemiology; Veterinary Immunology; Veterinary Medicine; Veterinary Microbiology (excl. Virology); Veterinary Parasitology; Veterinary Pathology; Veterinary Pharmacology; Veterinary Surgery; Veterinary Virology; Veterinary Sciences not elsewhere classified; marine epidemiology; parameter estimation; compartmental model; ABC method; global sensitivity analysis; basic reproduction number R0; Crassostrea gigas; oyster mortality; Pacific
• Online Access: https://doi.org/10.3389/fvets.2019.00142.s001; https://figshare.com/articles/Data_Sheet_1_Modeling_the_Transmission_of_Vibrio_aestuarianus_in_Pacific_Oysters_Using_Experimental_Infection_Data_docx/8121785

Vibrio aestuarianus is a bacterium related to mortality outbreaks in Pacific oysters, Crassostrea gigas, in France, Ireland, and Scotland since 2011. Knowledge about its transmission dynamics is still lacking, impairing guidance to prevent and control the related disease spread. Mathematical modeling is a relevant approach to better understand the determinants of a disease and predict its dynamics in imperfectly observed pathosystems. We developed here the first marine epidemiological model to estimate the key parameters of V. aestuarianus infection at a local scale in a small and closed oyster population under controlled laboratory conditions. Using a compartmental model accounting for free-living bacteria in seawater, we predicted the infection dynamics using dedicated and model-driven collected laboratory experimental transmission data. We estimated parameters and showed that waterborne transmission of V. aestuarianus is possible under experimental conditions, with a basic reproduction number R 0 of 2.88 (95% CI: 1.86; 3.35), and a generation time of 5.5 days. Our results highlighted a bacterial dose–dependent transmission of vibriosis at local scale. Global sensitivity analyses indicated that the bacteria shedding rate, the concentration of bacteria in seawater that yields a 50% chance of catching the infection, and the initial bacterial exposure dose W 0 were three critical parameters explaining most of the variation in the selected model outputs related to disease spread, i.e., R 0 , the maximum prevalence, oyster survival curve, and bacteria concentration in seawater. Prevention and control should target the exposure of oysters to bacterial concentration in seawater. This combined laboratory–modeling approach enabled us to maximize the use of information obtained through experiments. The identified key epidemiological parameters should be better refined by further dedicated laboratory experiments. These results revealed the importance of multidisciplinary approaches to gain consistent insights into the ...