Data_Sheet_1_CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.PDF
Due to their ability to present foreign antigens and prime naïve T cells, macrophages, and dendritic cells (DCs) are referred to as professional antigen-presenting cells (APCs). Although activated macrophages may function as APCs, these cells are particularly effective at directly engaging pathogens...
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ftfrontimediafig:oai:figshare.com:article/7835531 2023-05-15T15:32:10+02:00 Data_Sheet_1_CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.PDF Dimitar B. Iliev Leidy Lagos Hanna L. Thim Sven M. Jørgensen Aleksei Krasnov Jorunn B. Jørgensen 2019-03-13T04:09:33Z https://doi.org/10.3389/fimmu.2019.00378.s001 https://figshare.com/articles/Data_Sheet_1_CpGs_Induce_Differentiation_of_Atlantic_Salmon_Mononuclear_Phagocytes_Into_Cells_With_Dendritic_Morphology_and_a_Proinflammatory_Transcriptional_Profile_but_an_Exhausted_Allostimulatory_Activity_PDF/7835531 unknown doi:10.3389/fimmu.2019.00378.s001 https://figshare.com/articles/Data_Sheet_1_CpGs_Induce_Differentiation_of_Atlantic_Salmon_Mononuclear_Phagocytes_Into_Cells_With_Dendritic_Morphology_and_a_Proinflammatory_Transcriptional_Profile_but_an_Exhausted_Allostimulatory_Activity_PDF/7835531 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology mononuclear phagocytes antigen-presenting cells dendritic cells CpG oligodeoxynucleotides Atlantic salmon teleosts Dataset 2019 ftfrontimediafig https://doi.org/10.3389/fimmu.2019.00378.s001 2019-03-13T23:58:31Z Due to their ability to present foreign antigens and prime naïve T cells, macrophages, and dendritic cells (DCs) are referred to as professional antigen-presenting cells (APCs). Although activated macrophages may function as APCs, these cells are particularly effective at directly engaging pathogens through phagocytosis, and production of antimicrobial compounds. On the other hand, DCs possess superb antigen-presenting and costimulatory capacity and they are essential for commencement and regulation of adaptive immune responses. In in vitro models, development of mature mammalian DCs from monocytes requires sequential exposure to growth factors (including GM-CSF and IL-4) and proinflammatory stimuli such as toll-like receptor (TLR) ligands. Currently, except for IL-4/13, neither orthologs nor functional analogs of the growth factors which are essential for the differentiation of mammalian DCs (including GM-CSF and FLT3) have been identified in teleosts and data about differentiation of piscine APCs is scant. In the present study, primary salmon mononuclear phagocytes (MPs) stimulated in vitro for 5–7 days with a B-class CpG oligodeoxynucleotides (ODN 2006PS) underwent morphological differentiation and developed “dendritic” morphology, characterized by long, branching pseudopodia. Transcriptional profiling showed that these cells expressed high levels of proinflammatory mediators characteristic for M1 polarized MPs. However, the cells treated with CpGs for 7 days downregulated their surface MHCII molecules as well as their capacity to endocytose ovalbumin and exhibited attenuated allostimulatory activity. This concurred with transcriptional downregulation of costimulatory CD80/86 and upregulation of inhibitory CD274 (B7-H1) genes. Despite their exhausted allostimulatory activity, these cells were still responsive to re-stimulation with gardiquimod (a TLR7/8 ligand) and further upregulated a wide array of immune genes including proinflammatory mediators such as intereukin-1 beta and tumor necrosis factor. Overall, ... Dataset Atlantic salmon Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology mononuclear phagocytes antigen-presenting cells dendritic cells CpG oligodeoxynucleotides Atlantic salmon teleosts |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology mononuclear phagocytes antigen-presenting cells dendritic cells CpG oligodeoxynucleotides Atlantic salmon teleosts Dimitar B. Iliev Leidy Lagos Hanna L. Thim Sven M. Jørgensen Aleksei Krasnov Jorunn B. Jørgensen Data_Sheet_1_CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.PDF |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology mononuclear phagocytes antigen-presenting cells dendritic cells CpG oligodeoxynucleotides Atlantic salmon teleosts |
description |
Due to their ability to present foreign antigens and prime naïve T cells, macrophages, and dendritic cells (DCs) are referred to as professional antigen-presenting cells (APCs). Although activated macrophages may function as APCs, these cells are particularly effective at directly engaging pathogens through phagocytosis, and production of antimicrobial compounds. On the other hand, DCs possess superb antigen-presenting and costimulatory capacity and they are essential for commencement and regulation of adaptive immune responses. In in vitro models, development of mature mammalian DCs from monocytes requires sequential exposure to growth factors (including GM-CSF and IL-4) and proinflammatory stimuli such as toll-like receptor (TLR) ligands. Currently, except for IL-4/13, neither orthologs nor functional analogs of the growth factors which are essential for the differentiation of mammalian DCs (including GM-CSF and FLT3) have been identified in teleosts and data about differentiation of piscine APCs is scant. In the present study, primary salmon mononuclear phagocytes (MPs) stimulated in vitro for 5–7 days with a B-class CpG oligodeoxynucleotides (ODN 2006PS) underwent morphological differentiation and developed “dendritic” morphology, characterized by long, branching pseudopodia. Transcriptional profiling showed that these cells expressed high levels of proinflammatory mediators characteristic for M1 polarized MPs. However, the cells treated with CpGs for 7 days downregulated their surface MHCII molecules as well as their capacity to endocytose ovalbumin and exhibited attenuated allostimulatory activity. This concurred with transcriptional downregulation of costimulatory CD80/86 and upregulation of inhibitory CD274 (B7-H1) genes. Despite their exhausted allostimulatory activity, these cells were still responsive to re-stimulation with gardiquimod (a TLR7/8 ligand) and further upregulated a wide array of immune genes including proinflammatory mediators such as intereukin-1 beta and tumor necrosis factor. Overall, ... |
format |
Dataset |
author |
Dimitar B. Iliev Leidy Lagos Hanna L. Thim Sven M. Jørgensen Aleksei Krasnov Jorunn B. Jørgensen |
author_facet |
Dimitar B. Iliev Leidy Lagos Hanna L. Thim Sven M. Jørgensen Aleksei Krasnov Jorunn B. Jørgensen |
author_sort |
Dimitar B. Iliev |
title |
Data_Sheet_1_CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.PDF |
title_short |
Data_Sheet_1_CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.PDF |
title_full |
Data_Sheet_1_CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.PDF |
title_fullStr |
Data_Sheet_1_CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.PDF |
title_full_unstemmed |
Data_Sheet_1_CpGs Induce Differentiation of Atlantic Salmon Mononuclear Phagocytes Into Cells With Dendritic Morphology and a Proinflammatory Transcriptional Profile but an Exhausted Allostimulatory Activity.PDF |
title_sort |
data_sheet_1_cpgs induce differentiation of atlantic salmon mononuclear phagocytes into cells with dendritic morphology and a proinflammatory transcriptional profile but an exhausted allostimulatory activity.pdf |
publishDate |
2019 |
url |
https://doi.org/10.3389/fimmu.2019.00378.s001 https://figshare.com/articles/Data_Sheet_1_CpGs_Induce_Differentiation_of_Atlantic_Salmon_Mononuclear_Phagocytes_Into_Cells_With_Dendritic_Morphology_and_a_Proinflammatory_Transcriptional_Profile_but_an_Exhausted_Allostimulatory_Activity_PDF/7835531 |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_relation |
doi:10.3389/fimmu.2019.00378.s001 https://figshare.com/articles/Data_Sheet_1_CpGs_Induce_Differentiation_of_Atlantic_Salmon_Mononuclear_Phagocytes_Into_Cells_With_Dendritic_Morphology_and_a_Proinflammatory_Transcriptional_Profile_but_an_Exhausted_Allostimulatory_Activity_PDF/7835531 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2019.00378.s001 |
_version_ |
1766362664906260480 |