Data_Sheet_1_Melt Procedure Affects the Photosynthetic Response of Sea Ice Algae.pdf
The accuracy of sea ice algal production estimates is influenced by the range of melting procedures used in studies to obtain a liquid sample for incubation, particularly in relation to the duration of melt and the approach to buffering for osmotic shock. In this research, ice algal photophysiology...
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ftfrontimediafig:oai:figshare.com:article/7764713 2023-05-15T15:03:37+02:00 Data_Sheet_1_Melt Procedure Affects the Photosynthetic Response of Sea Ice Algae.pdf Karley Campbell C. J. Mundy Andrew R. Juhl Laura A. Dalman Christine Michel Ryan J. Galley Brent E. Else Nicolas X. Geilfus Søren Rysgaard 2019-02-25T13:57:16Z https://doi.org/10.3389/feart.2019.00021.s001 https://figshare.com/articles/Data_Sheet_1_Melt_Procedure_Affects_the_Photosynthetic_Response_of_Sea_Ice_Algae_pdf/7764713 unknown doi:10.3389/feart.2019.00021.s001 https://figshare.com/articles/Data_Sheet_1_Melt_Procedure_Affects_the_Photosynthetic_Response_of_Sea_Ice_Algae_pdf/7764713 CC BY 4.0 CC-BY Solid Earth Sciences Climate Science Atmospheric Sciences not elsewhere classified Exploration Geochemistry Inorganic Geochemistry Isotope Geochemistry Organic Geochemistry Geochemistry not elsewhere classified Igneous and Metamorphic Petrology Ore Deposit Petrology Palaeontology (incl. Palynology) Structural Geology Tectonics Volcanology Geology not elsewhere classified Seismology and Seismic Exploration Glaciology Hydrogeology Natural Hazards Quaternary Environments Earth Sciences not elsewhere classified Evolutionary Impacts of Climate Change sea ice algae salinity stress photophysiology sample melt Arctic Dataset 2019 ftfrontimediafig https://doi.org/10.3389/feart.2019.00021.s001 2019-02-27T23:59:01Z The accuracy of sea ice algal production estimates is influenced by the range of melting procedures used in studies to obtain a liquid sample for incubation, particularly in relation to the duration of melt and the approach to buffering for osmotic shock. In this research, ice algal photophysiology from 14 C incubations was compared in field samples prepared by three melt procedures: (i) a rapid ≤ 4 h melt of the bottommost ( < 1 cm) ice algal layer scraped into a large volume of filtered seawater (salinity 27–30), (ii) melt of a bottom 5 cm section diluted into a moderate volume of filtered seawater over 24 h (salinity 20–24), and (iii) melt of a bottom 5 cm section without any filtered seawater dilution over about 48 h (salinity 10–12). Maximum photosynthetic rate, photosynthetic efficiency and production at zero irradiance were significantly affected by the melt treatment employed in experiments. All variables were greatest in the highly diluted scrape sample and lowest in the bulk-ice samples melted in the absence of filtered seawater. Laboratory experiments exposing cultures of the common sea ice diatom Nitzschia frigida to different salinities and light conditions suggested that the field-based responses can be attributed to the rapid ( < 4 h) adverse effects of exposing cells to low salinities during melt without dilution. The observed differences in primary production between melt treatments were estimated to account for over 60% of the variability in production estimates reported for the Arctic. Future studies are strongly encouraged to replicate salinity conditions representative of in situ values during the melting process to minimize hypoosmotic stress, thereby most accurately estimating primary production. Dataset Arctic Climate change ice algae Sea ice Frontiers: Figshare Arctic |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Solid Earth Sciences Climate Science Atmospheric Sciences not elsewhere classified Exploration Geochemistry Inorganic Geochemistry Isotope Geochemistry Organic Geochemistry Geochemistry not elsewhere classified Igneous and Metamorphic Petrology Ore Deposit Petrology Palaeontology (incl. Palynology) Structural Geology Tectonics Volcanology Geology not elsewhere classified Seismology and Seismic Exploration Glaciology Hydrogeology Natural Hazards Quaternary Environments Earth Sciences not elsewhere classified Evolutionary Impacts of Climate Change sea ice algae salinity stress photophysiology sample melt Arctic |
spellingShingle |
Solid Earth Sciences Climate Science Atmospheric Sciences not elsewhere classified Exploration Geochemistry Inorganic Geochemistry Isotope Geochemistry Organic Geochemistry Geochemistry not elsewhere classified Igneous and Metamorphic Petrology Ore Deposit Petrology Palaeontology (incl. Palynology) Structural Geology Tectonics Volcanology Geology not elsewhere classified Seismology and Seismic Exploration Glaciology Hydrogeology Natural Hazards Quaternary Environments Earth Sciences not elsewhere classified Evolutionary Impacts of Climate Change sea ice algae salinity stress photophysiology sample melt Arctic Karley Campbell C. J. Mundy Andrew R. Juhl Laura A. Dalman Christine Michel Ryan J. Galley Brent E. Else Nicolas X. Geilfus Søren Rysgaard Data_Sheet_1_Melt Procedure Affects the Photosynthetic Response of Sea Ice Algae.pdf |
topic_facet |
Solid Earth Sciences Climate Science Atmospheric Sciences not elsewhere classified Exploration Geochemistry Inorganic Geochemistry Isotope Geochemistry Organic Geochemistry Geochemistry not elsewhere classified Igneous and Metamorphic Petrology Ore Deposit Petrology Palaeontology (incl. Palynology) Structural Geology Tectonics Volcanology Geology not elsewhere classified Seismology and Seismic Exploration Glaciology Hydrogeology Natural Hazards Quaternary Environments Earth Sciences not elsewhere classified Evolutionary Impacts of Climate Change sea ice algae salinity stress photophysiology sample melt Arctic |
description |
The accuracy of sea ice algal production estimates is influenced by the range of melting procedures used in studies to obtain a liquid sample for incubation, particularly in relation to the duration of melt and the approach to buffering for osmotic shock. In this research, ice algal photophysiology from 14 C incubations was compared in field samples prepared by three melt procedures: (i) a rapid ≤ 4 h melt of the bottommost ( < 1 cm) ice algal layer scraped into a large volume of filtered seawater (salinity 27–30), (ii) melt of a bottom 5 cm section diluted into a moderate volume of filtered seawater over 24 h (salinity 20–24), and (iii) melt of a bottom 5 cm section without any filtered seawater dilution over about 48 h (salinity 10–12). Maximum photosynthetic rate, photosynthetic efficiency and production at zero irradiance were significantly affected by the melt treatment employed in experiments. All variables were greatest in the highly diluted scrape sample and lowest in the bulk-ice samples melted in the absence of filtered seawater. Laboratory experiments exposing cultures of the common sea ice diatom Nitzschia frigida to different salinities and light conditions suggested that the field-based responses can be attributed to the rapid ( < 4 h) adverse effects of exposing cells to low salinities during melt without dilution. The observed differences in primary production between melt treatments were estimated to account for over 60% of the variability in production estimates reported for the Arctic. Future studies are strongly encouraged to replicate salinity conditions representative of in situ values during the melting process to minimize hypoosmotic stress, thereby most accurately estimating primary production. |
format |
Dataset |
author |
Karley Campbell C. J. Mundy Andrew R. Juhl Laura A. Dalman Christine Michel Ryan J. Galley Brent E. Else Nicolas X. Geilfus Søren Rysgaard |
author_facet |
Karley Campbell C. J. Mundy Andrew R. Juhl Laura A. Dalman Christine Michel Ryan J. Galley Brent E. Else Nicolas X. Geilfus Søren Rysgaard |
author_sort |
Karley Campbell |
title |
Data_Sheet_1_Melt Procedure Affects the Photosynthetic Response of Sea Ice Algae.pdf |
title_short |
Data_Sheet_1_Melt Procedure Affects the Photosynthetic Response of Sea Ice Algae.pdf |
title_full |
Data_Sheet_1_Melt Procedure Affects the Photosynthetic Response of Sea Ice Algae.pdf |
title_fullStr |
Data_Sheet_1_Melt Procedure Affects the Photosynthetic Response of Sea Ice Algae.pdf |
title_full_unstemmed |
Data_Sheet_1_Melt Procedure Affects the Photosynthetic Response of Sea Ice Algae.pdf |
title_sort |
data_sheet_1_melt procedure affects the photosynthetic response of sea ice algae.pdf |
publishDate |
2019 |
url |
https://doi.org/10.3389/feart.2019.00021.s001 https://figshare.com/articles/Data_Sheet_1_Melt_Procedure_Affects_the_Photosynthetic_Response_of_Sea_Ice_Algae_pdf/7764713 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Climate change ice algae Sea ice |
genre_facet |
Arctic Climate change ice algae Sea ice |
op_relation |
doi:10.3389/feart.2019.00021.s001 https://figshare.com/articles/Data_Sheet_1_Melt_Procedure_Affects_the_Photosynthetic_Response_of_Sea_Ice_Algae_pdf/7764713 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/feart.2019.00021.s001 |
_version_ |
1766335473018470400 |