Data_Sheet_1_Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats.docx
Microbial mats are ubiquitous in polar freshwater ecosystems and sustain high concentrations of biomass despite the extreme seasonal variations in light and temperature. Here we aimed to resolve genomic adaptations for light-harvesting, bright-light protection, and carbon flow in mats that undergo s...
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ftfrontimediafig:oai:figshare.com:article/7413806 2023-05-15T14:59:13+02:00 Data_Sheet_1_Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats.docx Adrien Vigneron Perrine Cruaud Vani Mohit Marie-Josée Martineau Alexander I. Culley Connie Lovejoy Warwick F. Vincent 2018-12-04T04:05:51Z https://doi.org/10.3389/fmicb.2018.02881.s001 https://figshare.com/articles/Data_Sheet_1_Multiple_Strategies_for_Light-Harvesting_Photoprotection_and_Carbon_Flow_in_High_Latitude_Microbial_Mats_docx/7413806 unknown doi:10.3389/fmicb.2018.02881.s001 https://figshare.com/articles/Data_Sheet_1_Multiple_Strategies_for_Light-Harvesting_Photoprotection_and_Carbon_Flow_in_High_Latitude_Microbial_Mats_docx/7413806 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Arctic microbiome cyanobacteria rhodopsin pigment biofilm Dataset 2018 ftfrontimediafig https://doi.org/10.3389/fmicb.2018.02881.s001 2018-12-05T23:59:00Z Microbial mats are ubiquitous in polar freshwater ecosystems and sustain high concentrations of biomass despite the extreme seasonal variations in light and temperature. Here we aimed to resolve genomic adaptations for light-harvesting, bright-light protection, and carbon flow in mats that undergo seasonal freeze-up. To bracket a range of communities in shallow water habitats, we sampled cyanobacterial mats in the thawed littoral zone of two lakes situated at the northern and southern limits of the Canadian Arctic permafrost zone. We applied a multiphasic approach using pigment profiles from high performance liquid chromatography, Illumina MiSeq sequencing of the 16S and 18S rRNA genes, and metagenomic analysis. The mats shared a taxonomic and functional core microbiome, dominated by oxygenic cyanobacteria with light-harvesting and photoprotective pigments, bacteria with bacteriochlorophyll, and bacteria with light-driven Type I rhodopsins. Organisms able to use light for energy related processes represented up to 85% of the total microbial community, with 15–30% attributable to cyanobacteria and 55–70% attributable to other bacteria. The proportion of genes involved in anaplerotic CO 2 fixation was greater than for genes associated with oxygenic photosynthesis. Diverse heterotrophic bacteria, eukaryotes (including metazoans and fungi) and viruses co-occurred in both communities. The results indicate a broad range of strategies for capturing sunlight and CO 2 , and for the subsequent flow of energy and carbon in these complex, light-driven microbial ecosystems. Dataset Arctic permafrost Frontiers: Figshare Arctic |
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Open Polar |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Arctic microbiome cyanobacteria rhodopsin pigment biofilm |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Arctic microbiome cyanobacteria rhodopsin pigment biofilm Adrien Vigneron Perrine Cruaud Vani Mohit Marie-Josée Martineau Alexander I. Culley Connie Lovejoy Warwick F. Vincent Data_Sheet_1_Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats.docx |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Arctic microbiome cyanobacteria rhodopsin pigment biofilm |
description |
Microbial mats are ubiquitous in polar freshwater ecosystems and sustain high concentrations of biomass despite the extreme seasonal variations in light and temperature. Here we aimed to resolve genomic adaptations for light-harvesting, bright-light protection, and carbon flow in mats that undergo seasonal freeze-up. To bracket a range of communities in shallow water habitats, we sampled cyanobacterial mats in the thawed littoral zone of two lakes situated at the northern and southern limits of the Canadian Arctic permafrost zone. We applied a multiphasic approach using pigment profiles from high performance liquid chromatography, Illumina MiSeq sequencing of the 16S and 18S rRNA genes, and metagenomic analysis. The mats shared a taxonomic and functional core microbiome, dominated by oxygenic cyanobacteria with light-harvesting and photoprotective pigments, bacteria with bacteriochlorophyll, and bacteria with light-driven Type I rhodopsins. Organisms able to use light for energy related processes represented up to 85% of the total microbial community, with 15–30% attributable to cyanobacteria and 55–70% attributable to other bacteria. The proportion of genes involved in anaplerotic CO 2 fixation was greater than for genes associated with oxygenic photosynthesis. Diverse heterotrophic bacteria, eukaryotes (including metazoans and fungi) and viruses co-occurred in both communities. The results indicate a broad range of strategies for capturing sunlight and CO 2 , and for the subsequent flow of energy and carbon in these complex, light-driven microbial ecosystems. |
format |
Dataset |
author |
Adrien Vigneron Perrine Cruaud Vani Mohit Marie-Josée Martineau Alexander I. Culley Connie Lovejoy Warwick F. Vincent |
author_facet |
Adrien Vigneron Perrine Cruaud Vani Mohit Marie-Josée Martineau Alexander I. Culley Connie Lovejoy Warwick F. Vincent |
author_sort |
Adrien Vigneron |
title |
Data_Sheet_1_Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats.docx |
title_short |
Data_Sheet_1_Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats.docx |
title_full |
Data_Sheet_1_Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats.docx |
title_fullStr |
Data_Sheet_1_Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats.docx |
title_full_unstemmed |
Data_Sheet_1_Multiple Strategies for Light-Harvesting, Photoprotection, and Carbon Flow in High Latitude Microbial Mats.docx |
title_sort |
data_sheet_1_multiple strategies for light-harvesting, photoprotection, and carbon flow in high latitude microbial mats.docx |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmicb.2018.02881.s001 https://figshare.com/articles/Data_Sheet_1_Multiple_Strategies_for_Light-Harvesting_Photoprotection_and_Carbon_Flow_in_High_Latitude_Microbial_Mats_docx/7413806 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic permafrost |
genre_facet |
Arctic permafrost |
op_relation |
doi:10.3389/fmicb.2018.02881.s001 https://figshare.com/articles/Data_Sheet_1_Multiple_Strategies_for_Light-Harvesting_Photoprotection_and_Carbon_Flow_in_High_Latitude_Microbial_Mats_docx/7413806 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2018.02881.s001 |
_version_ |
1766331338293510144 |