Data_Sheet_1_Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation.fasta
Parasites are key drivers of phytoplankton bloom dynamics and related aquatic ecosystem processes. Yet, the dearth of morphological and molecular information hinders the assessment of their diversity and ecological role. Using single-cell techniques, we characterise morphologically and molecularly,...
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ftfrontimediafig:oai:figshare.com:article/7410107 2023-05-15T17:30:50+02:00 Data_Sheet_1_Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation.fasta Andrea Garvetto Elisabeth Nézan Yacine Badis Gwenael Bilien Paola Arce Eileen Bresnan Claire M. M. Gachon Raffaele Siano 2018-12-03T04:15:50Z https://doi.org/10.3389/fmicb.2018.02918.s001 https://figshare.com/articles/Data_Sheet_1_Novel_Widespread_Marine_Oomycetes_Parasitising_Diatoms_Including_the_Toxic_Genus_Pseudo-nitzschia_Genetic_Morphological_and_Ecological_Characterisation_fasta/7410107 unknown doi:10.3389/fmicb.2018.02918.s001 https://figshare.com/articles/Data_Sheet_1_Novel_Widespread_Marine_Oomycetes_Parasitising_Diatoms_Including_the_Toxic_Genus_Pseudo-nitzschia_Genetic_Morphological_and_Ecological_Characterisation_fasta/7410107 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Pseudo-nitzschia diatoms marine oomycetes plankton parasites single-cell analysis metabarcoding Dataset 2018 ftfrontimediafig https://doi.org/10.3389/fmicb.2018.02918.s001 2018-12-05T23:59:18Z Parasites are key drivers of phytoplankton bloom dynamics and related aquatic ecosystem processes. Yet, the dearth of morphological and molecular information hinders the assessment of their diversity and ecological role. Using single-cell techniques, we characterise morphologically and molecularly, intracellular parasitoids infecting four potentially toxin-producing Pseudo-nitzschia and one Melosira species on the North Atlantic coast. These sequences define two, morphologically indistinguishable clades within the phylum Oomycota, related to the genera of algal parasites Anisolpidium and Olpidiopsis and the diatom parasitoid species Miracula helgolandica. Our morphological data are insufficient to attribute either clade to the still unsequenced genus Ectrogella; hence it is proposed to name the clades OOM_1 and OOM_2. A screening of global databases of the barcode regions V4 and V9 of the 18S rDNA demonstrate the presence of these parasitoids beyond the North Atlantic coastal region. During a biweekly metabarcoding survey (Concarneau Bay, France), reads associated with one sequenced parasitoid coincided with the decline of Cerataulina pelagica bloom, whilst the other parasitoids co-occurred at low abundance with Pseudo-nitzschia. Our data highlight a complex and unexplored diversity of the oomycete parasitoids of diatoms and calls for the investigation of their phenology, evolution, and potential contribution in controlling their host spatial-temporal dynamics. Dataset North Atlantic Frontiers: Figshare |
institution |
Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Pseudo-nitzschia diatoms marine oomycetes plankton parasites single-cell analysis metabarcoding |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Pseudo-nitzschia diatoms marine oomycetes plankton parasites single-cell analysis metabarcoding Andrea Garvetto Elisabeth Nézan Yacine Badis Gwenael Bilien Paola Arce Eileen Bresnan Claire M. M. Gachon Raffaele Siano Data_Sheet_1_Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation.fasta |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Pseudo-nitzschia diatoms marine oomycetes plankton parasites single-cell analysis metabarcoding |
description |
Parasites are key drivers of phytoplankton bloom dynamics and related aquatic ecosystem processes. Yet, the dearth of morphological and molecular information hinders the assessment of their diversity and ecological role. Using single-cell techniques, we characterise morphologically and molecularly, intracellular parasitoids infecting four potentially toxin-producing Pseudo-nitzschia and one Melosira species on the North Atlantic coast. These sequences define two, morphologically indistinguishable clades within the phylum Oomycota, related to the genera of algal parasites Anisolpidium and Olpidiopsis and the diatom parasitoid species Miracula helgolandica. Our morphological data are insufficient to attribute either clade to the still unsequenced genus Ectrogella; hence it is proposed to name the clades OOM_1 and OOM_2. A screening of global databases of the barcode regions V4 and V9 of the 18S rDNA demonstrate the presence of these parasitoids beyond the North Atlantic coastal region. During a biweekly metabarcoding survey (Concarneau Bay, France), reads associated with one sequenced parasitoid coincided with the decline of Cerataulina pelagica bloom, whilst the other parasitoids co-occurred at low abundance with Pseudo-nitzschia. Our data highlight a complex and unexplored diversity of the oomycete parasitoids of diatoms and calls for the investigation of their phenology, evolution, and potential contribution in controlling their host spatial-temporal dynamics. |
format |
Dataset |
author |
Andrea Garvetto Elisabeth Nézan Yacine Badis Gwenael Bilien Paola Arce Eileen Bresnan Claire M. M. Gachon Raffaele Siano |
author_facet |
Andrea Garvetto Elisabeth Nézan Yacine Badis Gwenael Bilien Paola Arce Eileen Bresnan Claire M. M. Gachon Raffaele Siano |
author_sort |
Andrea Garvetto |
title |
Data_Sheet_1_Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation.fasta |
title_short |
Data_Sheet_1_Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation.fasta |
title_full |
Data_Sheet_1_Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation.fasta |
title_fullStr |
Data_Sheet_1_Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation.fasta |
title_full_unstemmed |
Data_Sheet_1_Novel Widespread Marine Oomycetes Parasitising Diatoms, Including the Toxic Genus Pseudo-nitzschia: Genetic, Morphological, and Ecological Characterisation.fasta |
title_sort |
data_sheet_1_novel widespread marine oomycetes parasitising diatoms, including the toxic genus pseudo-nitzschia: genetic, morphological, and ecological characterisation.fasta |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmicb.2018.02918.s001 https://figshare.com/articles/Data_Sheet_1_Novel_Widespread_Marine_Oomycetes_Parasitising_Diatoms_Including_the_Toxic_Genus_Pseudo-nitzschia_Genetic_Morphological_and_Ecological_Characterisation_fasta/7410107 |
genre |
North Atlantic |
genre_facet |
North Atlantic |
op_relation |
doi:10.3389/fmicb.2018.02918.s001 https://figshare.com/articles/Data_Sheet_1_Novel_Widespread_Marine_Oomycetes_Parasitising_Diatoms_Including_the_Toxic_Genus_Pseudo-nitzschia_Genetic_Morphological_and_Ecological_Characterisation_fasta/7410107 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2018.02918.s001 |
_version_ |
1766127931649687552 |