Video_2_Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.MOV

Rhodococcus fascians BD8, isolated from Arctic soil, was found to produce biosurfactant when grown on n-hexadecane as the sole carbon source. The glycolipid product was identified as the trehalose lipid with a molecular mass of 848 g mol −1 . The purified biosurfactant reduced the surface tension of...

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Main Authors: Tomasz Janek, Anna Krasowska, Żaneta Czyżnikowska, Marcin Łukaszewicz
Format: Dataset
Language:unknown
Published: 2018
Subjects:
Online Access:https://doi.org/10.3389/fmicb.2018.02441.s003
https://figshare.com/articles/Video_2_Trehalose_Lipid_Biosurfactant_Reduces_Adhesion_of_Microbial_Pathogens_to_Polystyrene_and_Silicone_Surfaces_An_Experimental_and_Computational_Approach_MOV/7211918
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spelling ftfrontimediafig:oai:figshare.com:article/7211918 2023-05-15T15:16:05+02:00 Video_2_Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.MOV Tomasz Janek Anna Krasowska Żaneta Czyżnikowska Marcin Łukaszewicz 2018-10-16T08:33:56Z https://doi.org/10.3389/fmicb.2018.02441.s003 https://figshare.com/articles/Video_2_Trehalose_Lipid_Biosurfactant_Reduces_Adhesion_of_Microbial_Pathogens_to_Polystyrene_and_Silicone_Surfaces_An_Experimental_and_Computational_Approach_MOV/7211918 unknown doi:10.3389/fmicb.2018.02441.s003 https://figshare.com/articles/Video_2_Trehalose_Lipid_Biosurfactant_Reduces_Adhesion_of_Microbial_Pathogens_to_Polystyrene_and_Silicone_Surfaces_An_Experimental_and_Computational_Approach_MOV/7211918 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology biosurfactant trehalose lipid intermolecular interaction energy adhesion biofilm microbial pathogens Dataset Media 2018 ftfrontimediafig https://doi.org/10.3389/fmicb.2018.02441.s003 2018-10-17T22:57:27Z Rhodococcus fascians BD8, isolated from Arctic soil, was found to produce biosurfactant when grown on n-hexadecane as the sole carbon source. The glycolipid product was identified as the trehalose lipid with a molecular mass of 848 g mol −1 . The purified biosurfactant reduced the surface tension of water from 72 to 34 mN m −1 . The critical micelle concentration of trehalose lipid was 0.140 mg mL −1 . To examine its potential for biomedical applications, the antimicrobial and antiadhesive activity of the biosurfactant was evaluated against several pathogenic microorganisms. Trehalose lipid showed antimicrobial activity against resistant pathogens. The largest antimicrobial activities of trehalose lipid were observed against Vibrio harveyi and Proteus vulgaris. The highest concentration tested (0.5 mg mL −1 ) caused a partial (11–34%) inhibition of other Gram-positive and Gram-negative bacteria and 30% inhibition of Candida albicans growth. The trehalose lipid also showed significant antiadhesive properties against all of the tested microorganisms to polystyrene surface and silicone urethral catheters. The biosurfactant showed 95 and 70% antiadhesive activity against C. albicans and Escherichia coli, respectively. Finally, the role and application of trehalose lipid as an antiadhesive compound was investigated by the modification of the polystyrene and silicone surfaces. The intermolecular interaction energy calculations were performed for investigated complexes at the density functional level of theory. The results indicate that the presence of aromatic moieties can be substantial in the stabilization of trehalose lipid-surface complexes. The antimicrobial and antiadhesive activities of trehalose lipid make them promising alternatives to synthetic surfactants in a wide range of medical applications. Based on our findings, we propose that, because of its ability to inhibit microbial colonization of polystyrene and silicone surfaces, trehalose lipid can be used as a surface coating agent. Dataset Arctic Frontiers: Figshare Arctic
institution Open Polar
collection Frontiers: Figshare
op_collection_id ftfrontimediafig
language unknown
topic Microbiology
Microbial Genetics
Microbial Ecology
Mycology
biosurfactant
trehalose lipid
intermolecular interaction energy
adhesion
biofilm
microbial pathogens
spellingShingle Microbiology
Microbial Genetics
Microbial Ecology
Mycology
biosurfactant
trehalose lipid
intermolecular interaction energy
adhesion
biofilm
microbial pathogens
Tomasz Janek
Anna Krasowska
Żaneta Czyżnikowska
Marcin Łukaszewicz
Video_2_Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.MOV
topic_facet Microbiology
Microbial Genetics
Microbial Ecology
Mycology
biosurfactant
trehalose lipid
intermolecular interaction energy
adhesion
biofilm
microbial pathogens
description Rhodococcus fascians BD8, isolated from Arctic soil, was found to produce biosurfactant when grown on n-hexadecane as the sole carbon source. The glycolipid product was identified as the trehalose lipid with a molecular mass of 848 g mol −1 . The purified biosurfactant reduced the surface tension of water from 72 to 34 mN m −1 . The critical micelle concentration of trehalose lipid was 0.140 mg mL −1 . To examine its potential for biomedical applications, the antimicrobial and antiadhesive activity of the biosurfactant was evaluated against several pathogenic microorganisms. Trehalose lipid showed antimicrobial activity against resistant pathogens. The largest antimicrobial activities of trehalose lipid were observed against Vibrio harveyi and Proteus vulgaris. The highest concentration tested (0.5 mg mL −1 ) caused a partial (11–34%) inhibition of other Gram-positive and Gram-negative bacteria and 30% inhibition of Candida albicans growth. The trehalose lipid also showed significant antiadhesive properties against all of the tested microorganisms to polystyrene surface and silicone urethral catheters. The biosurfactant showed 95 and 70% antiadhesive activity against C. albicans and Escherichia coli, respectively. Finally, the role and application of trehalose lipid as an antiadhesive compound was investigated by the modification of the polystyrene and silicone surfaces. The intermolecular interaction energy calculations were performed for investigated complexes at the density functional level of theory. The results indicate that the presence of aromatic moieties can be substantial in the stabilization of trehalose lipid-surface complexes. The antimicrobial and antiadhesive activities of trehalose lipid make them promising alternatives to synthetic surfactants in a wide range of medical applications. Based on our findings, we propose that, because of its ability to inhibit microbial colonization of polystyrene and silicone surfaces, trehalose lipid can be used as a surface coating agent.
format Dataset
author Tomasz Janek
Anna Krasowska
Żaneta Czyżnikowska
Marcin Łukaszewicz
author_facet Tomasz Janek
Anna Krasowska
Żaneta Czyżnikowska
Marcin Łukaszewicz
author_sort Tomasz Janek
title Video_2_Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.MOV
title_short Video_2_Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.MOV
title_full Video_2_Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.MOV
title_fullStr Video_2_Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.MOV
title_full_unstemmed Video_2_Trehalose Lipid Biosurfactant Reduces Adhesion of Microbial Pathogens to Polystyrene and Silicone Surfaces: An Experimental and Computational Approach.MOV
title_sort video_2_trehalose lipid biosurfactant reduces adhesion of microbial pathogens to polystyrene and silicone surfaces: an experimental and computational approach.mov
publishDate 2018
url https://doi.org/10.3389/fmicb.2018.02441.s003
https://figshare.com/articles/Video_2_Trehalose_Lipid_Biosurfactant_Reduces_Adhesion_of_Microbial_Pathogens_to_Polystyrene_and_Silicone_Surfaces_An_Experimental_and_Computational_Approach_MOV/7211918
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_relation doi:10.3389/fmicb.2018.02441.s003
https://figshare.com/articles/Video_2_Trehalose_Lipid_Biosurfactant_Reduces_Adhesion_of_Microbial_Pathogens_to_Polystyrene_and_Silicone_Surfaces_An_Experimental_and_Computational_Approach_MOV/7211918
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fmicb.2018.02441.s003
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