Data_Sheet_1_The Role of the Vascular Endothelial Growth Factor (VEGF) Signaling in Biomineralization of the Oyster Crassostrea gigas.PDF

Vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGFR) play a role in early development, organogenesis, and regeneration, as well as biomineralization of invertebrates. The involvement of VEGF and VEGFR in biomineralization was demonstrated in sea urchin la...

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Main Authors: Anna V. Ivanina, Ballav Borah, Tadas Rimkevicius, Jason Macrander, Helen Piontkivska, Inna M. Sokolova, Elia Beniash
Format: Dataset
Language:unknown
Published: 2018
Subjects:
Online Access:https://doi.org/10.3389/fmars.2018.00309.s001
https://figshare.com/articles/Data_Sheet_1_The_Role_of_the_Vascular_Endothelial_Growth_Factor_VEGF_Signaling_in_Biomineralization_of_the_Oyster_Crassostrea_gigas_PDF/7015637
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spelling ftfrontimediafig:oai:figshare.com:article/7015637 2023-05-15T15:59:05+02:00 Data_Sheet_1_The Role of the Vascular Endothelial Growth Factor (VEGF) Signaling in Biomineralization of the Oyster Crassostrea gigas.PDF Anna V. Ivanina Ballav Borah Tadas Rimkevicius Jason Macrander Helen Piontkivska Inna M. Sokolova Elia Beniash 2018-08-28T04:03:52Z https://doi.org/10.3389/fmars.2018.00309.s001 https://figshare.com/articles/Data_Sheet_1_The_Role_of_the_Vascular_Endothelial_Growth_Factor_VEGF_Signaling_in_Biomineralization_of_the_Oyster_Crassostrea_gigas_PDF/7015637 unknown doi:10.3389/fmars.2018.00309.s001 https://figshare.com/articles/Data_Sheet_1_The_Role_of_the_Vascular_Endothelial_Growth_Factor_VEGF_Signaling_in_Biomineralization_of_the_Oyster_Crassostrea_gigas_PDF/7015637 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering biomineralization mollusk VEGF gene expression regulation hardness crystallinity respiration Dataset 2018 ftfrontimediafig https://doi.org/10.3389/fmars.2018.00309.s001 2018-08-29T22:57:14Z Vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGFR) play a role in early development, organogenesis, and regeneration, as well as biomineralization of invertebrates. The involvement of VEGF and VEGFR in biomineralization was demonstrated in sea urchin larvae but its role in the biomineralization of other invertebrate groups such as mollusks is not known. We assessed the potential role of VEGF signaling on biomineralization of a model marine bivalve, the Pacific oyster Crassostrea gigas, by analyzing the effects of a VEGFR inhibitor (50 nM axitinib) on shell growth, shell hardness, and expression profiles of biomineralization-related genes. Bioinformatics analysis identified a wide range of biomineralization-related genes potentially activated by VEGF including carbonic anhydrases (CAs), sodium/proton and sodium/calcium exchangers, calmodulins, and genes involved in chitin metabolism. Exposure to a VEGFR inhibitor axitinib led to upregulation of CAs (CA7 and CA12), sodium/proton exchanger 10, sodium/calcium exchanger 1 and 3, as well as calmodulin mRNA in hemocytes (HCs). In the mantle tissue, axitinib exposure led to a compensatory upregulation of VEGFR mRNA but did not affect the expression of other studied biomineralization genes. Expression of VEGF, VEGFR mRNA, or that of the downstream biomineralization-related genes in a non-biomineralizing tissue (the gill) was not affected by axitinib. The shell mass was higher in the axitinib treatment group. Inhibition of VEGFR led to significant increase in shell microhardness, and to a small but statistically significant decrease in crystallinity. Our results therefore indicate that, although VEGF signaling might not play a crucial role in shell biomineralization of C. gigas as it does in mammals and echinoderms, it is involved in regulation of shell formation in bivalves and that effects of VEGF on biomineralization are likely mediated by HCs. Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific
institution Open Polar
collection Frontiers: Figshare
op_collection_id ftfrontimediafig
language unknown
topic Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
biomineralization
mollusk
VEGF
gene expression regulation
hardness
crystallinity
respiration
spellingShingle Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
biomineralization
mollusk
VEGF
gene expression regulation
hardness
crystallinity
respiration
Anna V. Ivanina
Ballav Borah
Tadas Rimkevicius
Jason Macrander
Helen Piontkivska
Inna M. Sokolova
Elia Beniash
Data_Sheet_1_The Role of the Vascular Endothelial Growth Factor (VEGF) Signaling in Biomineralization of the Oyster Crassostrea gigas.PDF
topic_facet Oceanography
Marine Biology
Marine Geoscience
Biological Oceanography
Chemical Oceanography
Physical Oceanography
Marine Engineering
biomineralization
mollusk
VEGF
gene expression regulation
hardness
crystallinity
respiration
description Vascular endothelial growth factor (VEGF) and vascular endothelial growth factor receptor (VEGFR) play a role in early development, organogenesis, and regeneration, as well as biomineralization of invertebrates. The involvement of VEGF and VEGFR in biomineralization was demonstrated in sea urchin larvae but its role in the biomineralization of other invertebrate groups such as mollusks is not known. We assessed the potential role of VEGF signaling on biomineralization of a model marine bivalve, the Pacific oyster Crassostrea gigas, by analyzing the effects of a VEGFR inhibitor (50 nM axitinib) on shell growth, shell hardness, and expression profiles of biomineralization-related genes. Bioinformatics analysis identified a wide range of biomineralization-related genes potentially activated by VEGF including carbonic anhydrases (CAs), sodium/proton and sodium/calcium exchangers, calmodulins, and genes involved in chitin metabolism. Exposure to a VEGFR inhibitor axitinib led to upregulation of CAs (CA7 and CA12), sodium/proton exchanger 10, sodium/calcium exchanger 1 and 3, as well as calmodulin mRNA in hemocytes (HCs). In the mantle tissue, axitinib exposure led to a compensatory upregulation of VEGFR mRNA but did not affect the expression of other studied biomineralization genes. Expression of VEGF, VEGFR mRNA, or that of the downstream biomineralization-related genes in a non-biomineralizing tissue (the gill) was not affected by axitinib. The shell mass was higher in the axitinib treatment group. Inhibition of VEGFR led to significant increase in shell microhardness, and to a small but statistically significant decrease in crystallinity. Our results therefore indicate that, although VEGF signaling might not play a crucial role in shell biomineralization of C. gigas as it does in mammals and echinoderms, it is involved in regulation of shell formation in bivalves and that effects of VEGF on biomineralization are likely mediated by HCs.
format Dataset
author Anna V. Ivanina
Ballav Borah
Tadas Rimkevicius
Jason Macrander
Helen Piontkivska
Inna M. Sokolova
Elia Beniash
author_facet Anna V. Ivanina
Ballav Borah
Tadas Rimkevicius
Jason Macrander
Helen Piontkivska
Inna M. Sokolova
Elia Beniash
author_sort Anna V. Ivanina
title Data_Sheet_1_The Role of the Vascular Endothelial Growth Factor (VEGF) Signaling in Biomineralization of the Oyster Crassostrea gigas.PDF
title_short Data_Sheet_1_The Role of the Vascular Endothelial Growth Factor (VEGF) Signaling in Biomineralization of the Oyster Crassostrea gigas.PDF
title_full Data_Sheet_1_The Role of the Vascular Endothelial Growth Factor (VEGF) Signaling in Biomineralization of the Oyster Crassostrea gigas.PDF
title_fullStr Data_Sheet_1_The Role of the Vascular Endothelial Growth Factor (VEGF) Signaling in Biomineralization of the Oyster Crassostrea gigas.PDF
title_full_unstemmed Data_Sheet_1_The Role of the Vascular Endothelial Growth Factor (VEGF) Signaling in Biomineralization of the Oyster Crassostrea gigas.PDF
title_sort data_sheet_1_the role of the vascular endothelial growth factor (vegf) signaling in biomineralization of the oyster crassostrea gigas.pdf
publishDate 2018
url https://doi.org/10.3389/fmars.2018.00309.s001
https://figshare.com/articles/Data_Sheet_1_The_Role_of_the_Vascular_Endothelial_Growth_Factor_VEGF_Signaling_in_Biomineralization_of_the_Oyster_Crassostrea_gigas_PDF/7015637
geographic Pacific
geographic_facet Pacific
genre Crassostrea gigas
Pacific oyster
genre_facet Crassostrea gigas
Pacific oyster
op_relation doi:10.3389/fmars.2018.00309.s001
https://figshare.com/articles/Data_Sheet_1_The_Role_of_the_Vascular_Endothelial_Growth_Factor_VEGF_Signaling_in_Biomineralization_of_the_Oyster_Crassostrea_gigas_PDF/7015637
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fmars.2018.00309.s001
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