Table_1_Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.XLSX
The genus Carboxydocella forms a deeply branching family in the class Clostridia and is currently represented by three physiologically diverse species of thermophilic prokaryotes. The type strain of the type species, Carboxydocella thermautotrophica 41 T , is an obligate chemolithoautotroph growing...
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ftfrontimediafig:oai:figshare.com:article/6917138 2023-05-15T16:59:03+02:00 Table_1_Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.XLSX Stepan V. Toshchakov Alexander V. Lebedinsky Tatyana G. Sokolova Daria G. Zavarzina Alexei A. Korzhenkov Alina V. Teplyuk Natalia I. Chistyakova Vyacheslav S. Rusakov Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov Sergey N. Gavrilov 2018-08-03T04:19:20Z https://doi.org/10.3389/fmicb.2018.01759.s002 https://figshare.com/articles/Table_1_Genomic_Insights_Into_Energy_Metabolism_of_Carboxydocella_thermautotrophica_Coupling_Hydrogenogenic_CO_Oxidation_With_the_Reduction_of_Fe_III_Minerals_XLSX/6917138 unknown doi:10.3389/fmicb.2018.01759.s002 https://figshare.com/articles/Table_1_Genomic_Insights_Into_Energy_Metabolism_of_Carboxydocella_thermautotrophica_Coupling_Hydrogenogenic_CO_Oxidation_With_the_Reduction_of_Fe_III_Minerals_XLSX/6917138 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Carboxydocella thermophile Kamchatka hot springs genomics hydrogenogenic carboxydotrophy Fe(III) reduction Fe(III) silicate minerals Firmicutes Dataset 2018 ftfrontimediafig https://doi.org/10.3389/fmicb.2018.01759.s002 2018-08-08T22:58:03Z The genus Carboxydocella forms a deeply branching family in the class Clostridia and is currently represented by three physiologically diverse species of thermophilic prokaryotes. The type strain of the type species, Carboxydocella thermautotrophica 41 T , is an obligate chemolithoautotroph growing exclusively by hydrogenogenic CO oxidation. Another strain, isolated from a hot spring at Uzon caldera, Kamchatka in the course of this work, is capable of coupling carboxydotrophy and dissimilatory reduction of Fe(III) from oxic and phyllosilicate minerals. The processes of carboxydotrophy and Fe(III) reduction appeared to be interdependent in this strain. The genomes of both isolates were sequenced, assembled into single chromosome sequences (for strain 41 T a plasmid sequence was also assembled) and analyzed. Genome analysis revealed that each of the two strains possessed six genes encoding diverse Ni,Fe-containing CO dehydrogenases (maximum reported in complete prokaryotic genomes), indicating crucial role of carbon monoxide in C. thermautotrophica metabolism. Both strains possessed a set of 30 multiheme c-type cytochromes, but only the newly isolated Fe-reducing strain 019 had one extra gene of a 17-heme cytochrome, which is proposed to represent a novel determinant of dissimilatory iron reduction in prokaryotes. Mössbauer studies revealed that strain 019 induced reductive transformation of the abundant ferric/ferrous-mica mineral glauconite to siderite during carboxydotrophic growth. Reconstruction of the C. thermautotrophica strains energy metabolism is the first comprehensive genome analysis of a representative of the deep phylogenetic branch Clostridia Incertae Sedis, family V. Our data provide insights into energy metabolism of C. thermautotrophica with an emphasis on its ecological implications. Dataset Kamchatka Frontiers: Figshare |
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Frontiers: Figshare |
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Microbiology Microbial Genetics Microbial Ecology Mycology Carboxydocella thermophile Kamchatka hot springs genomics hydrogenogenic carboxydotrophy Fe(III) reduction Fe(III) silicate minerals Firmicutes |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Carboxydocella thermophile Kamchatka hot springs genomics hydrogenogenic carboxydotrophy Fe(III) reduction Fe(III) silicate minerals Firmicutes Stepan V. Toshchakov Alexander V. Lebedinsky Tatyana G. Sokolova Daria G. Zavarzina Alexei A. Korzhenkov Alina V. Teplyuk Natalia I. Chistyakova Vyacheslav S. Rusakov Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov Sergey N. Gavrilov Table_1_Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.XLSX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Carboxydocella thermophile Kamchatka hot springs genomics hydrogenogenic carboxydotrophy Fe(III) reduction Fe(III) silicate minerals Firmicutes |
description |
The genus Carboxydocella forms a deeply branching family in the class Clostridia and is currently represented by three physiologically diverse species of thermophilic prokaryotes. The type strain of the type species, Carboxydocella thermautotrophica 41 T , is an obligate chemolithoautotroph growing exclusively by hydrogenogenic CO oxidation. Another strain, isolated from a hot spring at Uzon caldera, Kamchatka in the course of this work, is capable of coupling carboxydotrophy and dissimilatory reduction of Fe(III) from oxic and phyllosilicate minerals. The processes of carboxydotrophy and Fe(III) reduction appeared to be interdependent in this strain. The genomes of both isolates were sequenced, assembled into single chromosome sequences (for strain 41 T a plasmid sequence was also assembled) and analyzed. Genome analysis revealed that each of the two strains possessed six genes encoding diverse Ni,Fe-containing CO dehydrogenases (maximum reported in complete prokaryotic genomes), indicating crucial role of carbon monoxide in C. thermautotrophica metabolism. Both strains possessed a set of 30 multiheme c-type cytochromes, but only the newly isolated Fe-reducing strain 019 had one extra gene of a 17-heme cytochrome, which is proposed to represent a novel determinant of dissimilatory iron reduction in prokaryotes. Mössbauer studies revealed that strain 019 induced reductive transformation of the abundant ferric/ferrous-mica mineral glauconite to siderite during carboxydotrophic growth. Reconstruction of the C. thermautotrophica strains energy metabolism is the first comprehensive genome analysis of a representative of the deep phylogenetic branch Clostridia Incertae Sedis, family V. Our data provide insights into energy metabolism of C. thermautotrophica with an emphasis on its ecological implications. |
format |
Dataset |
author |
Stepan V. Toshchakov Alexander V. Lebedinsky Tatyana G. Sokolova Daria G. Zavarzina Alexei A. Korzhenkov Alina V. Teplyuk Natalia I. Chistyakova Vyacheslav S. Rusakov Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov Sergey N. Gavrilov |
author_facet |
Stepan V. Toshchakov Alexander V. Lebedinsky Tatyana G. Sokolova Daria G. Zavarzina Alexei A. Korzhenkov Alina V. Teplyuk Natalia I. Chistyakova Vyacheslav S. Rusakov Elizaveta A. Bonch-Osmolovskaya Ilya V. Kublanov Sergey N. Gavrilov |
author_sort |
Stepan V. Toshchakov |
title |
Table_1_Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.XLSX |
title_short |
Table_1_Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.XLSX |
title_full |
Table_1_Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.XLSX |
title_fullStr |
Table_1_Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.XLSX |
title_full_unstemmed |
Table_1_Genomic Insights Into Energy Metabolism of Carboxydocella thermautotrophica Coupling Hydrogenogenic CO Oxidation With the Reduction of Fe(III) Minerals.XLSX |
title_sort |
table_1_genomic insights into energy metabolism of carboxydocella thermautotrophica coupling hydrogenogenic co oxidation with the reduction of fe(iii) minerals.xlsx |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmicb.2018.01759.s002 https://figshare.com/articles/Table_1_Genomic_Insights_Into_Energy_Metabolism_of_Carboxydocella_thermautotrophica_Coupling_Hydrogenogenic_CO_Oxidation_With_the_Reduction_of_Fe_III_Minerals_XLSX/6917138 |
genre |
Kamchatka |
genre_facet |
Kamchatka |
op_relation |
doi:10.3389/fmicb.2018.01759.s002 https://figshare.com/articles/Table_1_Genomic_Insights_Into_Energy_Metabolism_of_Carboxydocella_thermautotrophica_Coupling_Hydrogenogenic_CO_Oxidation_With_the_Reduction_of_Fe_III_Minerals_XLSX/6917138 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2018.01759.s002 |
_version_ |
1766051221600206848 |