Table_6_A Single Vibrionales 16S rRNA Oligotype Dominates the Intestinal Microbiome in Two Geographically Separated Atlantic cod Populations.XLSX
Atlantic cod (Gadus morhua) provides an interesting species for the study of host-microbe interactions because it lacks the MHC II complex that is involved in the presentation of extracellular pathogens. Nonetheless, little is known about the diversity of its microbiome in natural populations. Here,...
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ftfrontimediafig:oai:figshare.com:article/6814142 2023-05-15T15:27:07+02:00 Table_6_A Single Vibrionales 16S rRNA Oligotype Dominates the Intestinal Microbiome in Two Geographically Separated Atlantic cod Populations.XLSX Even S. Riiser Thomas H. A. Haverkamp Ørnulf Borgan Kjetill S. Jakobsen Sissel Jentoft Bastiaan Star 2018-07-13T04:11:07Z https://doi.org/10.3389/fmicb.2018.01561.s009 https://figshare.com/articles/Table_6_A_Single_Vibrionales_16S_rRNA_Oligotype_Dominates_the_Intestinal_Microbiome_in_Two_Geographically_Separated_Atlantic_cod_Populations_XLSX/6814142 unknown doi:10.3389/fmicb.2018.01561.s009 https://figshare.com/articles/Table_6_A_Single_Vibrionales_16S_rRNA_Oligotype_Dominates_the_Intestinal_Microbiome_in_Two_Geographically_Separated_Atlantic_cod_Populations_XLSX/6814142 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology Atlantic cod intestinal microbiome gut microbiome microbiota 16S rRNA Vibrionales Photobacterium Dataset 2018 ftfrontimediafig https://doi.org/10.3389/fmicb.2018.01561.s009 2018-07-18T22:57:57Z Atlantic cod (Gadus morhua) provides an interesting species for the study of host-microbe interactions because it lacks the MHC II complex that is involved in the presentation of extracellular pathogens. Nonetheless, little is known about the diversity of its microbiome in natural populations. Here, we use high-throughput sequencing of the 16S rRNA V4 region, amplified with the primer design of the Earth Microbiome Project (EMP), to investigate the microbial composition in gut content and mucosa of 22 adult individuals from two coastal populations in Norway, located 470 km apart. We identify a core microbiome of 23 OTUs (97% sequence similarity) in all individuals that comprises 93% of the total number of reads. The most abundant orders are classified as Vibrionales, Fusobacteriales, Clostridiales, and Bacteroidales. While mucosal samples show significantly lower diversity than gut content samples, no differences in OTU community composition are observed between the two geographically separated populations. All specimens share a limited number of abundant OTUs. Moreover, the most abundant OTU consists of a single oligotype (order Vibrionales, genus Photobacterium) that represents nearly 50% of the reads in both locations. Our results suggest that these microbiomes comprise a limited number of species or that the EMP V4 primers do not yield sufficient resolution to confidently separate these communities. Our study contributes to a growing body of literature that shows limited spatial differentiation of the intestinal microbiomes in marine fish based on 16S rRNA sequencing, highlighting the need for multi-gene approaches to provide more insight into the diversity of these communities. Dataset atlantic cod Gadus morhua Frontiers: Figshare Norway |
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Frontiers: Figshare |
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ftfrontimediafig |
language |
unknown |
topic |
Microbiology Microbial Genetics Microbial Ecology Mycology Atlantic cod intestinal microbiome gut microbiome microbiota 16S rRNA Vibrionales Photobacterium |
spellingShingle |
Microbiology Microbial Genetics Microbial Ecology Mycology Atlantic cod intestinal microbiome gut microbiome microbiota 16S rRNA Vibrionales Photobacterium Even S. Riiser Thomas H. A. Haverkamp Ørnulf Borgan Kjetill S. Jakobsen Sissel Jentoft Bastiaan Star Table_6_A Single Vibrionales 16S rRNA Oligotype Dominates the Intestinal Microbiome in Two Geographically Separated Atlantic cod Populations.XLSX |
topic_facet |
Microbiology Microbial Genetics Microbial Ecology Mycology Atlantic cod intestinal microbiome gut microbiome microbiota 16S rRNA Vibrionales Photobacterium |
description |
Atlantic cod (Gadus morhua) provides an interesting species for the study of host-microbe interactions because it lacks the MHC II complex that is involved in the presentation of extracellular pathogens. Nonetheless, little is known about the diversity of its microbiome in natural populations. Here, we use high-throughput sequencing of the 16S rRNA V4 region, amplified with the primer design of the Earth Microbiome Project (EMP), to investigate the microbial composition in gut content and mucosa of 22 adult individuals from two coastal populations in Norway, located 470 km apart. We identify a core microbiome of 23 OTUs (97% sequence similarity) in all individuals that comprises 93% of the total number of reads. The most abundant orders are classified as Vibrionales, Fusobacteriales, Clostridiales, and Bacteroidales. While mucosal samples show significantly lower diversity than gut content samples, no differences in OTU community composition are observed between the two geographically separated populations. All specimens share a limited number of abundant OTUs. Moreover, the most abundant OTU consists of a single oligotype (order Vibrionales, genus Photobacterium) that represents nearly 50% of the reads in both locations. Our results suggest that these microbiomes comprise a limited number of species or that the EMP V4 primers do not yield sufficient resolution to confidently separate these communities. Our study contributes to a growing body of literature that shows limited spatial differentiation of the intestinal microbiomes in marine fish based on 16S rRNA sequencing, highlighting the need for multi-gene approaches to provide more insight into the diversity of these communities. |
format |
Dataset |
author |
Even S. Riiser Thomas H. A. Haverkamp Ørnulf Borgan Kjetill S. Jakobsen Sissel Jentoft Bastiaan Star |
author_facet |
Even S. Riiser Thomas H. A. Haverkamp Ørnulf Borgan Kjetill S. Jakobsen Sissel Jentoft Bastiaan Star |
author_sort |
Even S. Riiser |
title |
Table_6_A Single Vibrionales 16S rRNA Oligotype Dominates the Intestinal Microbiome in Two Geographically Separated Atlantic cod Populations.XLSX |
title_short |
Table_6_A Single Vibrionales 16S rRNA Oligotype Dominates the Intestinal Microbiome in Two Geographically Separated Atlantic cod Populations.XLSX |
title_full |
Table_6_A Single Vibrionales 16S rRNA Oligotype Dominates the Intestinal Microbiome in Two Geographically Separated Atlantic cod Populations.XLSX |
title_fullStr |
Table_6_A Single Vibrionales 16S rRNA Oligotype Dominates the Intestinal Microbiome in Two Geographically Separated Atlantic cod Populations.XLSX |
title_full_unstemmed |
Table_6_A Single Vibrionales 16S rRNA Oligotype Dominates the Intestinal Microbiome in Two Geographically Separated Atlantic cod Populations.XLSX |
title_sort |
table_6_a single vibrionales 16s rrna oligotype dominates the intestinal microbiome in two geographically separated atlantic cod populations.xlsx |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmicb.2018.01561.s009 https://figshare.com/articles/Table_6_A_Single_Vibrionales_16S_rRNA_Oligotype_Dominates_the_Intestinal_Microbiome_in_Two_Geographically_Separated_Atlantic_cod_Populations_XLSX/6814142 |
geographic |
Norway |
geographic_facet |
Norway |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_relation |
doi:10.3389/fmicb.2018.01561.s009 https://figshare.com/articles/Table_6_A_Single_Vibrionales_16S_rRNA_Oligotype_Dominates_the_Intestinal_Microbiome_in_Two_Geographically_Separated_Atlantic_cod_Populations_XLSX/6814142 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fmicb.2018.01561.s009 |
_version_ |
1766357579444781056 |