Table_3_Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.XLS
As invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of th...
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ftfrontimediafig:oai:figshare.com:article/6480575 2023-05-15T15:58:33+02:00 Table_3_Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.XLS Shuai Jiang Limei Qiu Lingling Wang Zhihao Jia Zhao Lv Mengqiang Wang Conghui Liu Jiachao Xu Linsheng Song 2018-06-11T16:30:13Z https://doi.org/10.3389/fimmu.2018.01280.s004 https://figshare.com/articles/Table_3_Transcriptomic_and_Quantitative_Proteomic_Analyses_Provide_Insights_Into_the_Phagocytic_Killing_of_Hemocytes_in_the_Oyster_Crassostrea_gigas_XLS/6480575 unknown doi:10.3389/fimmu.2018.01280.s004 https://figshare.com/articles/Table_3_Transcriptomic_and_Quantitative_Proteomic_Analyses_Provide_Insights_Into_the_Phagocytic_Killing_of_Hemocytes_in_the_Oyster_Crassostrea_gigas_XLS/6480575 CC BY 4.0 CC-BY Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L Dataset 2018 ftfrontimediafig https://doi.org/10.3389/fimmu.2018.01280.s004 2018-06-13T22:56:56Z As invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of this type from the Pacific oyster Crassostrea gigas were classified efficiently in this study via fluorescence-activated cell sorting (FACS) based on their phagocytosis of FITC-labeled latex beads. Transcriptomic and quantitative proteomic analyses revealed a series of differentially expressed genes (DEGs) and proteins present in phagocytes; of the 352 significantly high expressed proteins identified here within the phagocyte proteome, 262 corresponding genes were similarly high expressed in the transcriptome, while 140 of 205 significantly low expressed proteins within the proteome were transcriptionally low expressed. A pathway crosstalk network analysis of these significantly high expressed proteins revealed that phagocytes were highly activated in a number of antimicrobial-related biological processes, including oxidation–reduction and lysosomal proteolysis processes. A number of DEGs, including oxidase, lysosomal protease, and immune receptors, were also validated in this study using quantitative PCR, while seven lysosomal cysteine proteases, referred to as cathepsin Ls, were significantly high expressed in phagocytes. Results show that the expression level of cathepsin L protein in phagocytes [mean fluorescence intensity (MFI): 327 ± 51] was significantly higher (p < 0.01) than that in non-phagocytic hemocytes (MFI: 83 ± 26), while the cathepsin L protein was colocalized with the phagocytosed Vibrio splendidus in oyster hemocytes during this process. The results of this study collectively suggest that oyster phagocytes possess both potent oxidative killing and microbial disintegration capacities; these findings provide important insights into hemocyte phagocytic killing as a component of C. gigas innate ... Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare Pacific |
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Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L Shuai Jiang Limei Qiu Lingling Wang Zhihao Jia Zhao Lv Mengqiang Wang Conghui Liu Jiachao Xu Linsheng Song Table_3_Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.XLS |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Crassostrea gigas transcriptome quantitative proteome phagocyte oxidative killing lysosome cathepsin L |
description |
As invertebrates lack an adaptive immune system, they depend to a large extent on their innate immune system to recognize and clear invading pathogens. Although phagocytes play pivotal roles in invertebrate innate immunity, the molecular mechanisms underlying this killing remain unclear. Cells of this type from the Pacific oyster Crassostrea gigas were classified efficiently in this study via fluorescence-activated cell sorting (FACS) based on their phagocytosis of FITC-labeled latex beads. Transcriptomic and quantitative proteomic analyses revealed a series of differentially expressed genes (DEGs) and proteins present in phagocytes; of the 352 significantly high expressed proteins identified here within the phagocyte proteome, 262 corresponding genes were similarly high expressed in the transcriptome, while 140 of 205 significantly low expressed proteins within the proteome were transcriptionally low expressed. A pathway crosstalk network analysis of these significantly high expressed proteins revealed that phagocytes were highly activated in a number of antimicrobial-related biological processes, including oxidation–reduction and lysosomal proteolysis processes. A number of DEGs, including oxidase, lysosomal protease, and immune receptors, were also validated in this study using quantitative PCR, while seven lysosomal cysteine proteases, referred to as cathepsin Ls, were significantly high expressed in phagocytes. Results show that the expression level of cathepsin L protein in phagocytes [mean fluorescence intensity (MFI): 327 ± 51] was significantly higher (p < 0.01) than that in non-phagocytic hemocytes (MFI: 83 ± 26), while the cathepsin L protein was colocalized with the phagocytosed Vibrio splendidus in oyster hemocytes during this process. The results of this study collectively suggest that oyster phagocytes possess both potent oxidative killing and microbial disintegration capacities; these findings provide important insights into hemocyte phagocytic killing as a component of C. gigas innate ... |
format |
Dataset |
author |
Shuai Jiang Limei Qiu Lingling Wang Zhihao Jia Zhao Lv Mengqiang Wang Conghui Liu Jiachao Xu Linsheng Song |
author_facet |
Shuai Jiang Limei Qiu Lingling Wang Zhihao Jia Zhao Lv Mengqiang Wang Conghui Liu Jiachao Xu Linsheng Song |
author_sort |
Shuai Jiang |
title |
Table_3_Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.XLS |
title_short |
Table_3_Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.XLS |
title_full |
Table_3_Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.XLS |
title_fullStr |
Table_3_Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.XLS |
title_full_unstemmed |
Table_3_Transcriptomic and Quantitative Proteomic Analyses Provide Insights Into the Phagocytic Killing of Hemocytes in the Oyster Crassostrea gigas.XLS |
title_sort |
table_3_transcriptomic and quantitative proteomic analyses provide insights into the phagocytic killing of hemocytes in the oyster crassostrea gigas.xls |
publishDate |
2018 |
url |
https://doi.org/10.3389/fimmu.2018.01280.s004 https://figshare.com/articles/Table_3_Transcriptomic_and_Quantitative_Proteomic_Analyses_Provide_Insights_Into_the_Phagocytic_Killing_of_Hemocytes_in_the_Oyster_Crassostrea_gigas_XLS/6480575 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2018.01280.s004 https://figshare.com/articles/Table_3_Transcriptomic_and_Quantitative_Proteomic_Analyses_Provide_Insights_Into_the_Phagocytic_Killing_of_Hemocytes_in_the_Oyster_Crassostrea_gigas_XLS/6480575 |
op_rights |
CC BY 4.0 |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.3389/fimmu.2018.01280.s004 |
_version_ |
1766394301078568960 |