Table9.DOCX
Sea Star Wasting Disease (SSWD) describes a suite of disease signs that affected >20 species of asteroid since 2013 along a broad geographic range from the Alaska Peninsula to Baja California. Previous work identified the Sea Star associated Densovirus (SSaDV) as the best candidate pathogen for S...
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ftfrontimediafig:oai:figshare.com:article/5955541 2023-05-15T17:04:43+02:00 Table9.DOCX Ian Hewson Kalia S. I. Bistolas Eva M. Quijano Cardé Jason B. Button Parker J. Foster Jacob M. Flanzenbaum Jan Kocian Chaunte K. Lewis 2018-03-07T06:38:00Z https://doi.org/10.3389/fmars.2018.00077.s026 https://figshare.com/articles/Table9_DOCX/5955541 unknown doi:10.3389/fmars.2018.00077.s026 https://figshare.com/articles/Table9_DOCX/5955541 CC BY 4.0 CC-BY Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering sea star wasting Asteroidea densovirus RNA virus pathogen Dataset 2018 ftfrontimediafig https://doi.org/10.3389/fmars.2018.00077.s026 2018-03-07T23:55:26Z Sea Star Wasting Disease (SSWD) describes a suite of disease signs that affected >20 species of asteroid since 2013 along a broad geographic range from the Alaska Peninsula to Baja California. Previous work identified the Sea Star associated Densovirus (SSaDV) as the best candidate pathogen for SSWD in three species of common asteroid (Pycnopodia helianthoides, Pisaster ochraceus, and Evasterias troscheli), and virus-sized material (<0.22 μm) elicited SSWD signs in P. helianthoides. However, the ability of virus-sized material to elicit SSWD in other species of asteroids was not known. Discordance between detection of SSaDV by qPCR and by viral metagenomics inspired the redesign of qPCR primers to encompass SSaDV and two densoviral genotypes detected in wasting asteroids. Analysis of asteroid samples collected during SSWD emergence in 2013–2014 showed an association between wasting asteroid-associated densoviruses (WAaDs) and SSWD in only one species (P. helianthoides). WAaDs were found in association with asymptomatic asteroids in contemporary (2016 and later) populations, suggesting that they may form subclinical infections at the times they were sampled. WAaDs were found in SSWD-affected P. helianthoides after being absent in asymptomatic individuals a year earlier at one location (Kodiak). Direct challenge of P. ochraceus, Pisaster brevispinus, and E. troscheli with virus-sized material from SSWD-affected individuals did not elicit SSWD in any trial. RNA viral genomes discovered in viral metagenomes and host transcriptomes had viral loads and metagenome fragment recruitment patterns that were inconsistent with SSWD. Analysis of water temperature and precipitation patterns on a regional scale suggests that SSWD occurred following dry conditions at several locations, but mostly was inconsistently associated with either parameter. Semi-continuous monitoring of SSWD subtidally at two sites in the Salish Sea from 2013 to 2017 indicated that SSWD in E. troscheli and P. ochraceus was associated with elevated ... Dataset Kodiak Alaska Frontiers: Figshare Baja |
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Open Polar |
collection |
Frontiers: Figshare |
op_collection_id |
ftfrontimediafig |
language |
unknown |
topic |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering sea star wasting Asteroidea densovirus RNA virus pathogen |
spellingShingle |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering sea star wasting Asteroidea densovirus RNA virus pathogen Ian Hewson Kalia S. I. Bistolas Eva M. Quijano Cardé Jason B. Button Parker J. Foster Jacob M. Flanzenbaum Jan Kocian Chaunte K. Lewis Table9.DOCX |
topic_facet |
Oceanography Marine Biology Marine Geoscience Biological Oceanography Chemical Oceanography Physical Oceanography Marine Engineering sea star wasting Asteroidea densovirus RNA virus pathogen |
description |
Sea Star Wasting Disease (SSWD) describes a suite of disease signs that affected >20 species of asteroid since 2013 along a broad geographic range from the Alaska Peninsula to Baja California. Previous work identified the Sea Star associated Densovirus (SSaDV) as the best candidate pathogen for SSWD in three species of common asteroid (Pycnopodia helianthoides, Pisaster ochraceus, and Evasterias troscheli), and virus-sized material (<0.22 μm) elicited SSWD signs in P. helianthoides. However, the ability of virus-sized material to elicit SSWD in other species of asteroids was not known. Discordance between detection of SSaDV by qPCR and by viral metagenomics inspired the redesign of qPCR primers to encompass SSaDV and two densoviral genotypes detected in wasting asteroids. Analysis of asteroid samples collected during SSWD emergence in 2013–2014 showed an association between wasting asteroid-associated densoviruses (WAaDs) and SSWD in only one species (P. helianthoides). WAaDs were found in association with asymptomatic asteroids in contemporary (2016 and later) populations, suggesting that they may form subclinical infections at the times they were sampled. WAaDs were found in SSWD-affected P. helianthoides after being absent in asymptomatic individuals a year earlier at one location (Kodiak). Direct challenge of P. ochraceus, Pisaster brevispinus, and E. troscheli with virus-sized material from SSWD-affected individuals did not elicit SSWD in any trial. RNA viral genomes discovered in viral metagenomes and host transcriptomes had viral loads and metagenome fragment recruitment patterns that were inconsistent with SSWD. Analysis of water temperature and precipitation patterns on a regional scale suggests that SSWD occurred following dry conditions at several locations, but mostly was inconsistently associated with either parameter. Semi-continuous monitoring of SSWD subtidally at two sites in the Salish Sea from 2013 to 2017 indicated that SSWD in E. troscheli and P. ochraceus was associated with elevated ... |
format |
Dataset |
author |
Ian Hewson Kalia S. I. Bistolas Eva M. Quijano Cardé Jason B. Button Parker J. Foster Jacob M. Flanzenbaum Jan Kocian Chaunte K. Lewis |
author_facet |
Ian Hewson Kalia S. I. Bistolas Eva M. Quijano Cardé Jason B. Button Parker J. Foster Jacob M. Flanzenbaum Jan Kocian Chaunte K. Lewis |
author_sort |
Ian Hewson |
title |
Table9.DOCX |
title_short |
Table9.DOCX |
title_full |
Table9.DOCX |
title_fullStr |
Table9.DOCX |
title_full_unstemmed |
Table9.DOCX |
title_sort |
table9.docx |
publishDate |
2018 |
url |
https://doi.org/10.3389/fmars.2018.00077.s026 https://figshare.com/articles/Table9_DOCX/5955541 |
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Baja |
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Baja |
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Kodiak Alaska |
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Kodiak Alaska |
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doi:10.3389/fmars.2018.00077.s026 https://figshare.com/articles/Table9_DOCX/5955541 |
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CC BY 4.0 |
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CC-BY |
op_doi |
https://doi.org/10.3389/fmars.2018.00077.s026 |
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