Table_1.PDF

As an integral part of the resident microbial community of fish intestinal tract, the mycobiota is expected to play important roles in health and disease resistance of the host. The composition of the diverse fungal communities, which colonize the intestine, is greatly influenced by the host, their...

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Main Authors: Prabhugouda Siriyappagouder, Viswanath Kiron, Jep Lokesh, Moger Rajeish, Martina Kopp, Jorge Fernandes
Format: Dataset
Language:unknown
Published: 2018
Subjects:
Online Access:https://doi.org/10.3389/fmicb.2018.00387.s002
https://figshare.com/articles/Table_1_PDF/5950630
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spelling ftfrontimediafig:oai:figshare.com:article/5950630 2023-05-15T15:45:23+02:00 Table_1.PDF Prabhugouda Siriyappagouder Viswanath Kiron Jep Lokesh Moger Rajeish Martina Kopp Jorge Fernandes 2018-03-06T04:20:47Z https://doi.org/10.3389/fmicb.2018.00387.s002 https://figshare.com/articles/Table_1_PDF/5950630 unknown doi:10.3389/fmicb.2018.00387.s002 https://figshare.com/articles/Table_1_PDF/5950630 CC BY 4.0 CC-BY Microbiology Microbial Genetics Microbial Ecology Mycology zebrafish mycobiota yeast fungal diversity internal transcribed spacer 2 (ITS2) Illumina MiSeq Dataset 2018 ftfrontimediafig https://doi.org/10.3389/fmicb.2018.00387.s002 2018-03-07T23:55:58Z As an integral part of the resident microbial community of fish intestinal tract, the mycobiota is expected to play important roles in health and disease resistance of the host. The composition of the diverse fungal communities, which colonize the intestine, is greatly influenced by the host, their diet and geographic origin. Studies of fungal communities are rare and the majority of previous studies have relied on culture-based methods. In particular, fungal communities in fish are also poorly characterized. The aim of this study was to provide an in-depth overview of the intestinal mycobiota in a model fish species (zebrafish, Danio rerio) and to determine differences in fungal composition between wild and captive specimens. We have profiled the intestinal mycobiota of wild-caught (Sharavati River, India), laboratory-reared (Bodø, Norway) and wild-caught-laboratory-kept (Uttara, India) zebrafish by sequencing the fungal internal transcribed spacer 2 region on the Illumina MiSeq platform. Wild fish were exposed to variable environmental factors, whereas both laboratory groups were kept in controlled conditions. There were also differences in husbandry practices at Bodø and Uttara, particularly diet. Zebrafish from Bodø were reared in the laboratory for over 10 generations, while wild-caught-laboratory-kept fish from Uttara were housed in the laboratory for only 2 months before sample collection. The intestine of zebrafish contained members of more than 15 fungal classes belonging to the phyla Ascomycota, Basidiomycota, and Zygomycota. Fungal species richness and diversity distinguished the wild-caught and laboratory-reared zebrafish communities. Wild-caught zebrafish-associated mycobiota comprised mainly Dothideomycetes in contrast to their Saccharomycetes-dominated laboratory-reared counterparts. The predominant Saccharomycetes in laboratory-reared fish belonged to the saprotrophic guild. Another characteristic feature of laboratory-reared fish was the significantly higher abundance of Cryptococcus ... Dataset Bodø Bodø Frontiers: Figshare Bodø ENVELOPE(14.405,14.405,67.280,67.280) Norway
institution Open Polar
collection Frontiers: Figshare
op_collection_id ftfrontimediafig
language unknown
topic Microbiology
Microbial Genetics
Microbial Ecology
Mycology
zebrafish
mycobiota
yeast
fungal diversity
internal transcribed spacer 2 (ITS2)
Illumina MiSeq
spellingShingle Microbiology
Microbial Genetics
Microbial Ecology
Mycology
zebrafish
mycobiota
yeast
fungal diversity
internal transcribed spacer 2 (ITS2)
Illumina MiSeq
Prabhugouda Siriyappagouder
Viswanath Kiron
Jep Lokesh
Moger Rajeish
Martina Kopp
Jorge Fernandes
Table_1.PDF
topic_facet Microbiology
Microbial Genetics
Microbial Ecology
Mycology
zebrafish
mycobiota
yeast
fungal diversity
internal transcribed spacer 2 (ITS2)
Illumina MiSeq
description As an integral part of the resident microbial community of fish intestinal tract, the mycobiota is expected to play important roles in health and disease resistance of the host. The composition of the diverse fungal communities, which colonize the intestine, is greatly influenced by the host, their diet and geographic origin. Studies of fungal communities are rare and the majority of previous studies have relied on culture-based methods. In particular, fungal communities in fish are also poorly characterized. The aim of this study was to provide an in-depth overview of the intestinal mycobiota in a model fish species (zebrafish, Danio rerio) and to determine differences in fungal composition between wild and captive specimens. We have profiled the intestinal mycobiota of wild-caught (Sharavati River, India), laboratory-reared (Bodø, Norway) and wild-caught-laboratory-kept (Uttara, India) zebrafish by sequencing the fungal internal transcribed spacer 2 region on the Illumina MiSeq platform. Wild fish were exposed to variable environmental factors, whereas both laboratory groups were kept in controlled conditions. There were also differences in husbandry practices at Bodø and Uttara, particularly diet. Zebrafish from Bodø were reared in the laboratory for over 10 generations, while wild-caught-laboratory-kept fish from Uttara were housed in the laboratory for only 2 months before sample collection. The intestine of zebrafish contained members of more than 15 fungal classes belonging to the phyla Ascomycota, Basidiomycota, and Zygomycota. Fungal species richness and diversity distinguished the wild-caught and laboratory-reared zebrafish communities. Wild-caught zebrafish-associated mycobiota comprised mainly Dothideomycetes in contrast to their Saccharomycetes-dominated laboratory-reared counterparts. The predominant Saccharomycetes in laboratory-reared fish belonged to the saprotrophic guild. Another characteristic feature of laboratory-reared fish was the significantly higher abundance of Cryptococcus ...
format Dataset
author Prabhugouda Siriyappagouder
Viswanath Kiron
Jep Lokesh
Moger Rajeish
Martina Kopp
Jorge Fernandes
author_facet Prabhugouda Siriyappagouder
Viswanath Kiron
Jep Lokesh
Moger Rajeish
Martina Kopp
Jorge Fernandes
author_sort Prabhugouda Siriyappagouder
title Table_1.PDF
title_short Table_1.PDF
title_full Table_1.PDF
title_fullStr Table_1.PDF
title_full_unstemmed Table_1.PDF
title_sort table_1.pdf
publishDate 2018
url https://doi.org/10.3389/fmicb.2018.00387.s002
https://figshare.com/articles/Table_1_PDF/5950630
long_lat ENVELOPE(14.405,14.405,67.280,67.280)
geographic Bodø
Norway
geographic_facet Bodø
Norway
genre Bodø
Bodø
genre_facet Bodø
Bodø
op_relation doi:10.3389/fmicb.2018.00387.s002
https://figshare.com/articles/Table_1_PDF/5950630
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fmicb.2018.00387.s002
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