DataSheet_1_Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus.docx
Introduction The culture of Pacific oysters (Crassostrea gigas) is of significant socio-economic importance in the U.S. Pacific Northwest and other temperate regions worldwide, with disease outbreaks acting as significant bottlenecks to the successful production of healthy seed larvae. Therefore, th...
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Online Access: | https://doi.org/10.3389/fimmu.2024.1380089.s001 https://figshare.com/articles/dataset/DataSheet_1_Immunomodulatory_effects_of_a_probiotic_combination_treatment_to_improve_the_survival_of_Pacific_oyster_Crassostrea_gigas_larvae_against_infection_by_Vibrio_coralliilyticus_docx/26212691 |
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ftfrontimediafig:oai:figshare.com:article/26212691 2024-09-15T18:03:13+00:00 DataSheet_1_Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus.docx Jennifer Hesser Ryan S. Mueller Chris Langdon Carla B. Schubiger 2024-07-09T07:39:59Z https://doi.org/10.3389/fimmu.2024.1380089.s001 https://figshare.com/articles/dataset/DataSheet_1_Immunomodulatory_effects_of_a_probiotic_combination_treatment_to_improve_the_survival_of_Pacific_oyster_Crassostrea_gigas_larvae_against_infection_by_Vibrio_coralliilyticus_docx/26212691 unknown doi:10.3389/fimmu.2024.1380089.s001 https://figshare.com/articles/dataset/DataSheet_1_Immunomodulatory_effects_of_a_probiotic_combination_treatment_to_improve_the_survival_of_Pacific_oyster_Crassostrea_gigas_larvae_against_infection_by_Vibrio_coralliilyticus_docx/26212691 CC BY 4.0 Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Pacific oyster larvae probiotics immune response Vibrio coralliilyticus aquaculture Dataset 2024 ftfrontimediafig https://doi.org/10.3389/fimmu.2024.1380089.s001 2024-08-19T06:19:43Z Introduction The culture of Pacific oysters (Crassostrea gigas) is of significant socio-economic importance in the U.S. Pacific Northwest and other temperate regions worldwide, with disease outbreaks acting as significant bottlenecks to the successful production of healthy seed larvae. Therefore, the current study aims to describe the mechanisms of a probiotic combination in improving the survival of C. gigas larvae. Specifically, we investigate changes in C. gigas larval gene expression in response to V. coralliilyticus infection with or without a pre-treatment of a novel probiotic combination. Methods Treatment groups consisted of replicates of Pacific oyster larvae exposed to a) a combination of four probiotic bacteria at a total concentration of 3.0 x 10 5 CFU/mL at 18 hours post-fertilization (hpf), b) pathogenic V. coralliilyticus RE22 at a concentration of 6.0 x 10 3 CFU/mL at 48 hpf, and c) the probiotic combination at 18 hpf and V. coralliilyticus RE22 at 48 hpf. RNA was extracted from washed larvae after 72 hpf, and transcriptome sequencing was used to identify significant differentially expressed genes (DEGs) within each treatment. Results Larvae challenged with V. coralliilyticus showed enhanced expression of genes responsible for inhibiting immune signaling (i.e., TNFAIP3, PSMD10) and inducing apoptosis (i.e., CDIP53). However, when pre-treated with the probiotic combination, these genes were no longer differentially expressed relative to untreated control larvae. Additionally, pre-treatment with the probiotic combination increased expression of immune signaling proteins and immune effectors (i.e., IL-17, MyD88). Apparent immunomodulation in response to probiotic treatment corresponds to an increase in the survival of C. gigas larvae infected with V. coralliilyticus by up to 82%. Discussion These results indicate that infection with V. coralliilyticus can suppress the larval immune response while also prompting cell death. Furthermore, the results suggest that the probiotic combination treatment ... Dataset Crassostrea gigas Pacific oyster Frontiers: Figshare |
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Frontiers: Figshare |
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ftfrontimediafig |
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unknown |
topic |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Pacific oyster larvae probiotics immune response Vibrio coralliilyticus aquaculture |
spellingShingle |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Pacific oyster larvae probiotics immune response Vibrio coralliilyticus aquaculture Jennifer Hesser Ryan S. Mueller Chris Langdon Carla B. Schubiger DataSheet_1_Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus.docx |
topic_facet |
Immunology Applied Immunology (incl. Antibody Engineering Xenotransplantation and T-cell Therapies) Autoimmunity Cellular Immunology Humoural Immunology and Immunochemistry Immunogenetics (incl. Genetic Immunology) Innate Immunity Transplantation Immunology Tumour Immunology Immunology not elsewhere classified Genetic Immunology Animal Immunology Veterinary Immunology Pacific oyster larvae probiotics immune response Vibrio coralliilyticus aquaculture |
description |
Introduction The culture of Pacific oysters (Crassostrea gigas) is of significant socio-economic importance in the U.S. Pacific Northwest and other temperate regions worldwide, with disease outbreaks acting as significant bottlenecks to the successful production of healthy seed larvae. Therefore, the current study aims to describe the mechanisms of a probiotic combination in improving the survival of C. gigas larvae. Specifically, we investigate changes in C. gigas larval gene expression in response to V. coralliilyticus infection with or without a pre-treatment of a novel probiotic combination. Methods Treatment groups consisted of replicates of Pacific oyster larvae exposed to a) a combination of four probiotic bacteria at a total concentration of 3.0 x 10 5 CFU/mL at 18 hours post-fertilization (hpf), b) pathogenic V. coralliilyticus RE22 at a concentration of 6.0 x 10 3 CFU/mL at 48 hpf, and c) the probiotic combination at 18 hpf and V. coralliilyticus RE22 at 48 hpf. RNA was extracted from washed larvae after 72 hpf, and transcriptome sequencing was used to identify significant differentially expressed genes (DEGs) within each treatment. Results Larvae challenged with V. coralliilyticus showed enhanced expression of genes responsible for inhibiting immune signaling (i.e., TNFAIP3, PSMD10) and inducing apoptosis (i.e., CDIP53). However, when pre-treated with the probiotic combination, these genes were no longer differentially expressed relative to untreated control larvae. Additionally, pre-treatment with the probiotic combination increased expression of immune signaling proteins and immune effectors (i.e., IL-17, MyD88). Apparent immunomodulation in response to probiotic treatment corresponds to an increase in the survival of C. gigas larvae infected with V. coralliilyticus by up to 82%. Discussion These results indicate that infection with V. coralliilyticus can suppress the larval immune response while also prompting cell death. Furthermore, the results suggest that the probiotic combination treatment ... |
format |
Dataset |
author |
Jennifer Hesser Ryan S. Mueller Chris Langdon Carla B. Schubiger |
author_facet |
Jennifer Hesser Ryan S. Mueller Chris Langdon Carla B. Schubiger |
author_sort |
Jennifer Hesser |
title |
DataSheet_1_Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus.docx |
title_short |
DataSheet_1_Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus.docx |
title_full |
DataSheet_1_Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus.docx |
title_fullStr |
DataSheet_1_Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus.docx |
title_full_unstemmed |
DataSheet_1_Immunomodulatory effects of a probiotic combination treatment to improve the survival of Pacific oyster (Crassostrea gigas) larvae against infection by Vibrio coralliilyticus.docx |
title_sort |
datasheet_1_immunomodulatory effects of a probiotic combination treatment to improve the survival of pacific oyster (crassostrea gigas) larvae against infection by vibrio coralliilyticus.docx |
publishDate |
2024 |
url |
https://doi.org/10.3389/fimmu.2024.1380089.s001 https://figshare.com/articles/dataset/DataSheet_1_Immunomodulatory_effects_of_a_probiotic_combination_treatment_to_improve_the_survival_of_Pacific_oyster_Crassostrea_gigas_larvae_against_infection_by_Vibrio_coralliilyticus_docx/26212691 |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
doi:10.3389/fimmu.2024.1380089.s001 https://figshare.com/articles/dataset/DataSheet_1_Immunomodulatory_effects_of_a_probiotic_combination_treatment_to_improve_the_survival_of_Pacific_oyster_Crassostrea_gigas_larvae_against_infection_by_Vibrio_coralliilyticus_docx/26212691 |
op_rights |
CC BY 4.0 |
op_doi |
https://doi.org/10.3389/fimmu.2024.1380089.s001 |
_version_ |
1810440735998083072 |